Method to convert N-terminal glutamine to pyroglutamate for characterization of recombinant monoclonal antibodies

Analytical Biochemistry

Volumn 436, Issue 1, 2013, Pages 10-12

  Xu, Wei ^a   Peng, Yan ^a   Wang, Fengqiang ^a   Paporello, Brittany ^a   Richardson, Daisy ^a   Liu, Hongcheng ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Glutamine; Glutaminyl peptide cyclotransferase; Monoclonal antibodies; Pyroglutamate

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PEPTIDES;

ENZYMATIC CONVERSIONS; FRACTION COLLECTION; GLUTAMINE; GLUTAMINYL; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; N-TERMINALS; PYROGLUTAMATE; RECOMBINANT MONOCLONAL ANTIBODY;

AMINO ACIDS;

GLUTAMINE; MONOCLONAL ANTIBODY; PYROGLUTAMIC ACID; RECOMBINANT ANTIBODY;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; CYCLIZATION; ENZYME SPECIFICITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; PH; PRIORITY JOURNAL; PROCESS DEVELOPMENT; TEMPERATURE;

ANTIBODIES, MONOCLONAL; BIOCATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYCLIZATION; GLUTAMINE; MASS SPECTROMETRY; PYRROLIDONECARBOXYLIC ACID; RECOMBINANT PROTEINS;

EID: 84874397693     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.01.017     Document Type: Article

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