A non-radioactive complement-dependent cytotoxicity assay for anti-CD20 monoclonal antibody

Journal of Immunological Methods

Volumn 202, Issue 2, 1997, Pages 163-171

  Gazzano Santoro, Hélène ^a   Ralph, Peter ^a   Ryskamp, Thomas C ^b   Chen, Anthony B ^a   Mukku, Venkat R ^a  

^a GENENTECH INC   (United States)

^b BIOGEN   (United States)

Author keywords

Alamar blue; antibody; complement; cytotoxicity

Indexed keywords

ALAMAR BLUE; CD20 ANTIGEN; MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; B LYMPHOCYTE; COMPLEMENT DEPENDENT CYTOTOXICITY; FLUORESCENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

ANTIBODIES, ANTI-IDIOTYPIC; ANTIBODIES, MONOCLONAL; ANTIGENS, CD20; B-LYMPHOCYTES; COLORING AGENTS; COMPLEMENT SYSTEM PROTEINS; CYTOTOXICITY TESTS, IMMUNOLOGIC; HUMANS; OXAZINES; RECOMBINANT FUSION PROTEINS; XANTHENES;

EID: 0031588898     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00002-1     Document Type: Article

References (12)

1. 3H-thymidine incorporation assay. J. Immunol. Methods 170, 211.
2. Ashkenazi, A., Marsters, S.A., Capon, D.J., Chamow, S.M., Figari, I.S., Pennica, D., Goeddel, D.V., Palladino, M.A. and Smith, D.H. (1991) Protection against endotoxic shock by a tumor necrosis factor receptor immunoadhesin. Proc. Natl. Acad. Sci. USA 88, 10535.
3. HER2 antibody for human cancer therapy. Proc. Natl. Acad. Sci. USA 89, 4285.
4. Liu, A.Y., Robinson, R. Jr., Murray, E.D., Ledbetter, J.A., Hellstroom, I. and Hellstrom, K.E. (1987) Production of a mouse-human chimeric monoclonal antibody to CD20 with potent Fc-dependent biologic activity. J. Immunol. 139, 3521.
5. Maloney, D.G., Liles, T.M., Czerwinski, D.K., Rosenberg, J., Grillo-Lopez, A. and Levy, R. (1994) Phase I clinical trial using escalating single-dose infusion of chimeric anti-CD20 monoclonal antibody (IDEC-C2B8) in patients with recurrent B-cell lymphoma. Blood 84, 2457.
6. McLaughlin, P., Grillo-Lopez, A.J., Czuczman, M., Link, B.K., Levy, B.K., Dillman, R.O., Ho, A., Bennett, J.M., Heyman, M.R., Schilder, R.J., Cabanillas, F., Rogers, J. and Dallaire, B.K. (1996) Preliminary report on a Phase III pivotal trial of the anti-CD20 antibody (Mab) IDEC-C2B8 in patients (Pts) with relapsed low-grade or follicular lymphoma (LG/F NHL). Proc. Am. Soc. Clin. Oncol. 15, 417.
7. Mosmann, T.J. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55.
8. Page, B., Page, M. and Noel, C. (1993) A new fluorometric assay for cytotoxicity measurements in vitro. Int. J. Oncol. 3, 473.
9. Presta, L.G., Lahr, S.J., Shields, R.L., Porter, J.P., Gorman, C.M., Fendly, B.M. and Jardieu, P.M. (1993) Humanization of an antibody directed against IgE. J. Immunol. 151, 2623.
10. Reff, M.E., Carner, K., Chambers, K.S., Chinn, P.C., Leonard, J.E., Raab, R., Newman, R.A., Hanna, N. and Anderson, D.R. (1994) Depletion of B cells in vivo by a chimeric mouse human monoclonal antibody to CD20. Blood 83, 435.
11. Tao, M.H., Smith, R.I.F and Morrison, S.L. (1993) Structural features of human immunoglobulin G that determine isotype-specific differences in complement activation. J. Exp. Med. 178, 661.
12. Tedder, T.F. and Engel, P. (1994) CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol. Today 15, 450.