Cation exchange-HPLC and mass spectrometry reveal C-terminal amidation of an IgG1 heavy chain

Analytical Biochemistry

Volumn 360, Issue 1, 2007, Pages 75-83

  Johnson, Keith A ^a   Paisley Flango, Kari ^a   Tangarone, Bruce S ^a   Porter, Thomas J ^a   Rouse, Jason C ^a  

^a PFIZER INC   (United States)

Author keywords

C terminal processing; Mass spectrometry; Proline amidation; Recombinant monoclonal antibody; Weak cation exchange; amidation

Indexed keywords

COVALENT BONDS; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PEPTIDES; POSITIVE IONS; SULFUR COMPOUNDS;

ACCURATE MASS MEASUREMENTS; AMIDATION; C-TERMINAL PEPTIDES; CATION EXCHANGES; COMBINED ANALYSIS; CYSTEINE ALKYLATIONS; RECOMBINANT MONOCLONAL ANTIBODY; STRUCTURAL HETEROGENEITY;

AMINO ACIDS;

AMINO ACID; CORTICOTROPIN[18-39]; DNA; GLYCINE; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN HEAVY CHAIN; LYSINE; MONOCLONAL ANTIBODY; PEPTIDE; PROLINE; RECOMBINANT ANTIBODY;

AMIDATION; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CATION EXCHANGE; CELL CULTURE; CHO CELL; DNA SEQUENCE; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; QUADRUPOLE MASS SPECTROMETRY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TIME OF FLIGHT MASS SPECTROMETRY;

EID: 33845450543     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.10.012     Document Type: Article

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