C-terminal lysine variants in fully human monoclonal antibodies: Investigation of test methods and possible causes

Biotechnology and Bioengineering

Volumn 100, Issue 6, 2008, Pages 1132-1143

  Dick Jr , Lawrence W ^a   Qiu, Difei ^a   Mahon, David ^a   Adamo, Michael ^a   Cheng, Kuang Chuan ^a  

^a MEDAREX INC   (United States)

Author keywords

Carboxypeptidase; IEF; LC MS; Lysine variants; Post translational modification

Indexed keywords

CARBOXYPEPTIDASE; CASE STUDIES; CHARGE VARIATIONS; HUMAN MONOCLONAL ANTIBODIES; IEF; ION-EXCHANGE CHROMATOGRAPHY; LC-MS; LYSINE VARIANTS; POST-TRANSLATIONAL MODIFICATION; PRODUCTION PROCESSES; TEST METHODS; TRYPSIN DIGESTION;

ALLERGIES; AMINO ACIDS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; ION CHROMATOGRAPHY; ION EXCHANGE; MERGERS AND ACQUISITIONS; MONOCLONAL ANTIBODIES; PORT TERMINALS; SURFACE PLASMON RESONANCE;

ANTIBODIES;

CARBOXYPEPTIDASE; CARBOXYPEPTIDASE B; LYSINE; MONOCLONAL ANTIBODY; TRYPSIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; B CELL HYBRIDOMA; CAPILLARY ISOELECTRIC FOCUSING; CARBOXY TERMINAL SEQUENCE; CHO CELL; DIGESTION; FEMALE; HUMAN; HUMAN CELL; HYBRIDOMA CELL CULTURE; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC FOCUSING; LIQUID CHROMATOGRAPHY; NONHUMAN; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BUFFERS; CARBOXYPEPTIDASE B; CELL CULTURE TECHNIQUES; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; ELECTROPHORESIS, CAPILLARY; FEMALE; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN HEAVY CHAINS; ISOELECTRIC FOCUSING; LYSINE; PEPTIDE MAPPING; PLANT PROTEINS; PROTEIN PROCESSING, POST-TRANSLATIONAL; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 48649090322     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21855     Document Type: Article

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