Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation

Bioresource Technology

Volumn 101, Issue 4, 2010, Pages 1318-1323

  Wang, Zhihao ^a   Wang, Yun ^a   Zhang, Dongxu ^a   Li, Jianghua ^a   Hua, Zhaozhe ^a   Du, Guocheng ^a,b   Chen, Jian ^a,b  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b JIANGNAN UNIVERSITY   (China)

Author keywords

Alcohol oxidase; Pichia pastoris; Polygalacturonate lyase (PGL); Proteolytic degradation; Sorbitol

Indexed keywords

ALCOHOL OXIDASE; CARBON SOURCE; CELL MORTALITY; CELL VIABILITY; CO-FEEDING; CONSTANT RATE; HETEROLOGOUS PROTEIN PRODUCTION; INDUCTION PERIODS; PICHIA PASTORIS; POLYGALACTURONATES; PROTEOLYTIC DEGRADATION; RECOMBINANT PROTEIN;

FEEDING; FERMENTATION; METHANOL; PROTEINS;

DEGRADATION;

ALCOHOL OXIDASE; ALKALINE POLYGALACTURONATE LYASE; LYASE; METHANOL; RECOMBINANT PROTEIN; SORBITOL; UNCLASSIFIED DRUG;

ALCOHOL; BIODEGRADATION; CARBON; CATALYST; ENZYME ACTIVITY; FERMENTATION; METHANOL; MICROBIAL ACTIVITY; PROTEIN;

ARTICLE; BIOMASS; CARBON SOURCE; CELL DEATH; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SYNTHESIS; FERMENTATION; FUNGAL STRAIN; NONHUMAN; PICHIA PASTORIS; PLASMID; PRIORITY JOURNAL; PROTEIN DEGRADATION;

ALCOHOL OXIDOREDUCTASES; ALKALIES; BIOMASS; FERMENTATION; INTRACELLULAR SPACE; METHANOL; MICROBIAL VIABILITY; PEPTIDE HYDROLASES; PICHIA; POLYSACCHARIDE-LYASES; PROTEIN PROCESSING, POST-TRANSLATIONAL; SORBITOL; TEMPERATURE; TIME FACTORS;

PICHIA PASTORIS;

EID: 71549114849     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2009.09.025     Document Type: Article

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