Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter

New Biotechnology

Volumn 25, Issue 6, 2009, Pages 396-403

  Resina, David ^a   Maurer, Michael ^a   Cos, Oriol ^a   Arnau, Carolina ^a   Carnicer, Marc ^a   Marx, Hans ^b,c   Gasser, Brigitte ^b   Valero, Francisco ^a   Mattanovich, Diethard ^b,c   Ferrer, Pau ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^c Fachhochschule Campus Wien   (Austria)

Author keywords

BiP; Fed batch cultivation; Formaldehyde dehydrogenase promoter; Gas1; Hac1; Pichia pastoris; Rhizopus oryzae lipase; Unfolded protein response

Indexed keywords

BIP; FED-BATCH CULTIVATION; FORMALDEHYDE DEHYDROGENASE PROMOTER; GAS1; HAC1; PICHIA PASTORIS; RHIZOPUS ORYZAE LIPASE; UNFOLDED PROTEIN RESPONSE;

CELL MEMBRANES; ENZYMES; FORMALDEHYDE; GENE ENCODING; STRAIN; YEAST;

PHYSIOLOGY;

BETA 1,3 GLUCANOSYLTRANSGLYCOSYLASE; NITROGEN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR HAC1; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; GAS1 GENE; GENE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN SECRETION; PROTEIN SYNTHESIS; RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE;

GENE EXPRESSION REGULATION; GENETIC ENHANCEMENT; LIPASE; MEMBRANE PROTEINS; NITROGEN; PICHIA; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE PROTEINS;

PICHIA PASTORIS; RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE;

EID: 70350547284     PISSN: 18716784     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nbt.2009.01.008     Document Type: Article

References (24)

1. Lin Cereghino J., and Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24 (2000) 45-66
2. Shen S.G., et al. A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris. Gene 216 (1998) 93-102
3. Cos O., et al. Heterologous production of Rhizopus oryzae lipase in Pichia pastoris using the alcohol oxidase and formaldehyde dehydrogenase promoters in batch and fed-batch cultures. Biochem. Eng. J. 26 (2005) 86-94
4. Resina D., et al. Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter. Biotechnol. Bioeng. 91 (2005) 760-767
5. Hohenblum H., et al. Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol. Bioeng. 85 (2004) 367-375
6. Gasser B., et al. Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb. Cell Fact. 7 (2008) 11
7. Gasser B., et al. Engineering Pichia pastoris for improved production of antibody fragments. Biotechnol. Bioeng. 94 (2006) 353-361
8. Inan M., et al. Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase. Biotechnol. Bioeng. 93 (2006) 771-778
9. Mattanovich D., et al. Stress in recombinant protein producing yeasts. J. Biotechnol. 113 (2004) 121-135
10. Gasser B., et al. Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts. Appl. Environ. Microbiol. 73 (2007) 6499-6507
11. Cos O., et al. Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures. J. Biotechnol. 116 (2005) 321-335
12. Resina D., et al. Transcriptional response of Pichia pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction. Microb. Cell Fact. 6 (2007) 21
13. Ferrer P., et al. Yeast cell permeabilizing β-1.3-glucanases: a tool for the integration of downstream processes and metabolic engineering applications to yeast. Biotechnol. Bioeng. 58 (1998) 321-324
14. Marx H., et al. Cloning, deletion and protein secretory phenotype of the GAS1 homologue of Pichia pastoris. FEMS Microb. Lett. 264 (2006) 40-47
15. Resina D., et al. Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. J. Biotechnol. 109 (2004) 103-113
16. Higgins D.R., et al. Small vectors for expression based on dominant drug resistance with direct multicopy selection. Methods Mol. Biol. 103 (1998) 41-53
17. Sinha J., et al. Improved production of recombinant ovine interferon-tau by Mut(+) strain of Pichia pastoris using an optimized methanol feed profile. Biotechnol. Prog. 19 (2003) 794-802
18. Zhang W.H., et al. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol. Bioeng. 70 (2000) 1-8
19. Maurer M., et al. Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microb. Cell Fact. 5 (2006) 37
20. Thorpe E.D., et al. Sorbitol as a non-repressing carbon source for fed-batch fermentation of recombinant Pichia pastoris. Biotecnol. Lett. 21 (1999) 669-672
21. Resina, D. (2006) Ph.D. Thesis. Universitat Autònoma de Barcelona, Catalonia, Spain
22. Zwart K.B., and Harder W. Regulation of the metabolism of some alkylated amines in the yeasts Candida utilis and Hansenula polymorpha. J. Gen. Microbiol. 129 (1983) 3157-3169
23. Beer H.D., et al. The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence. Biochem. J. 319 (1996) 351-359
24. Graf A., et al. Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays. BMC Genomics 9 (2008) 390