Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol

Yeast

Volumn 26, Issue 9, 2009, Pages 473-484

  Çelik, Eda ^a   Çalik, Pinar ^a   Oliver, Stephen G ^b  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Fed batch; Methanol; Optimization; Oxygen; Pichia pastoris; Sorbitol

Indexed keywords

ALCOHOL OXIDASE; LACTIC ACID; METHANOL; RECOMBINANT ERYTHROPOIETIN; RECOMBINANT PROTEIN; SORBITOL; ERYTHROPOIETIN; OXYGEN;

ARTICLE; BIOTECHNOLOGY; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; ENZYME SYNTHESIS; FED BATCH FERMENTATION; FUNGAL METABOLISM; FUNGUS GROWTH; NONHUMAN; OXYGEN CONSUMPTION; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN SYNTHESIS; YEAST; BIOREACTOR; EVALUATION; FERMENTATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; METHODOLOGY; MICROBIOLOGY; PICHIA;

PICHIA PASTORIS;

BIOREACTORS; ERYTHROPOIETIN; FERMENTATION; HUMANS; INDUSTRIAL MICROBIOLOGY; METHANOL; OXYGEN; PICHIA; RECOMBINANT PROTEINS; SORBITOL;

EID: 70349897510     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1679     Document Type: Article

References (34)

1. Azevedo AM, Cabral JMS, Prazeres DMF, et al. 2004. Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase. J Mol Catal B Enzymol 27: 37-45.
2. Bailey JE, Ollis DF. 1986. Biochemical Engineering Fundamentals. McGraw Hill: Singapore.
3. Bandyopadhyay B, Humprey AE. 1967. Dynamic measurement of the volumetric oxygen transfer coefficient in fermentation systems. Biotechnol Bioeng 9: 533-544.
4. Blum H, Beier H, Gross HJ. 1987. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99.
5. Bradford MM. 1976. A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248-254.
6. Brierley RA, Bussineau C, Kosson R, et al. 1990. Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme. Ann NY Acad Sci 589: 350-362. (Pubitemid 20255801)
7. Charoenrat T, Ketudat-Cairns M, Jahic M, et al. 2006. Increased total air pressure versus oxygen limitation for enhanced oxygen transfer and product formation in a Pichia pastoris recombinant protein process. Biochem Eng J 30: 205-211. (Pubitemid 43928828)
8. Chiruvolu V, Cregg JM, Meagher MM. 1997. Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fed-batch fermentations. Enzyme Microb Tech 21: 277-283. (Pubitemid 27448295)
9. Cos O, Resina D, Ferrer P, et al. 2005. Heterologous production of Rhizopus oryzae lipase in Pichia pastoris using the alcohol oxidase and formaldehyde dehydrogenase promoters in batch and fed-batch cultures. Biochem Eng J 26: 86-94. (Pubitemid 41355047)
10. Cunha AE, Clemente JJ, Gomes R, et al. 2004. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng 86: 458-467. (Pubitemid 38667956)
11. Çalik P, Çalik G, Özdamar TH. 1998. Oxygen transfer effects in serine alkaline protease fermentation by Bacillus licheniformis: use of citric acid as the carbon source. Enzyme Microb Tech 23: 451-461.
12. Çalik P, Çalik G, Özdamar TH. 2000. Oxygen transfer strategy and its regulation effects in serine alkaline protease production by Bacillus licheniformis. Biotechnol Bioeng 69: 301-311.
13. Çelik E, Çalik P, Halloran SM, Oliver SG. 2007. Production of recombinant human erythropoietin from Pichia pastoris and its structural analysis. J Appl Microbiol 103: 2084-2094. (Pubitemid 350191149)
14. Çelik E, Özbay N, Oktar N, Çalik P. 2008. Use of biodiesel by-product crude glycerol as the carbon source for fermentation processes by recombinant Pichia pastoris. Ind Eng Chem Res 47: 2985-2990. (Pubitemid 351747414)
15. Hellwig S, Emde F, Raven NPG, et al. 2001. Analysis of single chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentation. Biotechnol Bioeng 74: 344-352. (Pubitemid 32678668)
16. Hong F, Meinander NQ, Jonsson LJ. 2002. Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol Bioeng 79: 438-449. (Pubitemid 34847321)
17. Hu XQ, Chu J, Zhang Z, et al. 2008. Effects of different glycerol feeding strategies on S-adenosyl-l-methionine biosynthesis by PGAP-driven Pichia pastoris overexpressing methionine adenosyltransferase. J Biotechnol 137: 44-49.
18. Inan M, Meagher MM. 2001. Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris. J Biosci Bioeng 92: 585-589. (Pubitemid 34107180)
19. Isett K, George H, Herber W, Amanullah A. 2007. Twenty-four-well plate miniature bioreactor high-throughput system: assessment for microbial cultivations. Biotechnol Bioeng 98: 1017-1028. (Pubitemid 350205579)
20. İleri N, Çalik P. 2006. Effects of pH strategy on endo- and exometabolome profiles and sodium potassium hydrogen ports of β-lactamase producing Bacillus licheniformis. Biotechnol Progr 22: 411-419.
21. Jungo C, Rerat C, Marison IW, von Stockar U. 2006. Quantitative characterization of the regulation of the synthesis of alcohol oxidase and of the expression of recombinant avidin in a Pichia pastoris Mut+ strain. Enzyme Microb Tech 39: 936-944. (Pubitemid 43912901)
22. Jungo C, Schenk J, Pasquier M, et al. 2007. A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris. J Biotechnol 131: 57-66. (Pubitemid 47042930)
23. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
24. Ramchuran SO, Mateus B, Holst O, Karlsson EN. 2005. The methylotrophic yeast Pichia pastoris as a host for the expression and production of thermostable xylanase from the bacterium Rhodothermus marinus. FEMS Yeast Res 5: 839-850. (Pubitemid 40733230)
25. Ramon R, Ferrer P, Valero F. 2007. Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol 130: 39-46. (Pubitemid 46634651)
26. Schenk J, Balazs K, Jungo C, et al. 2008. Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut+ strain. Biotechnol Bioeng 99: 368-376. (Pubitemid 351158285)
27. Sinha J, Plantz BA, Inan M, Meagher MM. 2005. Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: case study with recombinant ovine interferon-τ. Biotechnol Bioeng 89: 102-112. (Pubitemid 40110988)
28. Sreekrishna K, Brankamp RG, Kroop KE, et al. 1997. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190: 55-62. (Pubitemid 27214270)
29. Stratton J, Chiruvolu V, Meagher M. 1998. High cell-density fermentation. In Methods in Molecular Biology: Pichia Protocols, Higgins DR, Cregg JM (eds). Humana Press: Totowa, NJ; 107-120.
30. Tschopp JF, Brust PF, Cregg JM, et al. 1987. Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res 15: 3859-3876.
31. Wegner G. 1990. Emerging applications of the methylotrophic yeasts. FEMS Microbiol Rev 7: 279-283.
32. Xie J, Zhou Q, Du P, Gan R, Ye Q. 2005. Use of different carbon sources in cultivation of recombinant Pichia pastoris for angiostatin production. Enzyme Microb Tech 36: 210-216. (Pubitemid 40039075)
33. Zhang W, Inan M, Meagher MM. 2000. Fermentation strategies for recombinant protein expression in the methylotrophic yeast Pichia pastoris. Biotechnol Bioproc Eng 5: 275-287.
34. Zhang W, Li ZJ, Agblevo FA. 2005. Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production. Process Biochem 40: 2073-2078. (Pubitemid 40257032)