Increasing the cell viability and heterologous protein expression of Pichia pastoris mutant deficient in PMR1 gene by culture condition optimization

Applied Microbiology and Biotechnology

Volumn 81, Issue 2, 2008, Pages 235-241

  Zhao, Hong Liang ^a   Xue, Chong ^a   Wang, Yang ^a   Yao, Xue Qin ^a   Liu, Zhi Min ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

Large scale fermentation; Pichia pastoris; PMR1 disruption

Indexed keywords

BIOCHEMICAL ENGINEERING; CONCENTRATION (PROCESS); FERMENTATION; METHANOL; OXYGEN; THEOREM PROVING;

CARBON SOURCE TRANSITIONS; CELL LYSISES; CELL VIABILITIES; CULTURE CONDITIONS; CULTURE TEMPERATURES; EXPRESSION LEVELS; FUSION PROTEINS; HETEROLOGOUS PROTEIN EXPRESSIONS; HUMAN SERUM ALBUMINS; IN VITRO; LARGE-SCALE FERMENTATION; OSMOTIC PRESSURES; PICHIA PASTORIS; PMR1 DISRUPTION; PROTEOLYTIC DEGRADATIONS; SALT CONCENTRATIONS;

CELL CULTURE;

ALPHA2B INTERFERON; CALCIUM; CALCIUM ION; HUMAN SERUM ALBUMIN; HYBRID PROTEIN; METHANOL; OXYGEN;

CONCENTRATION (COMPOSITION); FERMENTATION; GENE EXPRESSION; MUTATION; OPTIMIZATION; YEAST;

ARTICLE; BIOMASS FERMENTATION; CARBON SOURCE; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; CYTOLYSIS; FERMENTATION OPTIMIZATION; FUNGAL CELL CULTURE; FUNGAL GENE; FUNGUS GROWTH; FUNGUS MUTANT; GENE MUTATION; NONHUMAN; OSMOTIC PRESSURE; PICHIA PASTORIS; PMR1 GENE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN STABILITY; SUPERNATANT; TIME;

CALCIUM-TRANSPORTING ATPASES; CULTURE MEDIA; HUMANS; INTERFERON ALFA-2B; MICROBIAL VIABILITY; PICHIA; RECOMBINANT FUSION PROTEINS; SERUM ALBUMIN; TEMPERATURE; TIME FACTORS;

PICHIA PASTORIS;

EID: 55649107479     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1666-0     Document Type: Article

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