Measurement of sirtuin enzyme activity using a substrate-agnostic fluorometric nicotinamide assay

Methods in Molecular Biology

Volumn 1077, Issue , 2013, Pages 167-177

  Hubbard, Basil P ^a   Sinclair, David A ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Aging; Deacetylation; Debutyrylation; Demalonylation; Depropionylation; Desuccinylation; Fluorometric detection of ammonia; Glycohydrolase assay; HDAC; Nicotinamide assay; Ortho phthalaldehyde (OPT); PARP assay; SIRT1; Sirtuin assay; yPnc1

Indexed keywords

GLYCOSIDASE; NICOTINAMIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; SIRTUIN; SIRTUIN 1;

ARTICLE; ENZYME ACTIVITY; FLUOROMETRY; PRIORITY JOURNAL; PROTEIN CLEAVAGE;

ACETYLATION; FLUOROMETRY; HUMANS; KINETICS; LYSINE; MITOCHONDRIAL PROTEINS; NAD; NUCLEOTIDE TRANSPORT PROTEINS; PEPTIDE FRAGMENTS; SIRTUIN 1; SUBSTRATE SPECIFICITY;

EID: 84884173245     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-637-5_11     Document Type: Article

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