A continuous microplate assay for sirtuins and nicotinamide-producing enzymes

Analytical Biochemistry

Volumn 394, Issue 1, 2009, Pages 101-109

  Smith, Brian C ^a   Hallows, William C ^a   Denu, John M ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

Acetyl; CD38; Deacetylase; Glutamate dehydrogenase; NAD+; Nicotinamidase; Nicotinamide; Sir2; Sirt1; Sirtuin

Indexed keywords

AMMONIA; DRUG PRODUCTS; KINETIC PARAMETERS; MAMMALS;

ACETYL; CD38; DEACETYLASE; GLUTAMATE DEHYDROGENASE; NAD^+; NICOTINAMIDASE; NICOTINAMIDE; SIR2; SIRT1; SIRTUIN;

ENZYMES;

2 OXOGLUTARIC ACID; ACETYL COENZYME A SYNTHETASE; ADENOSINE DIPHOSPHATE RIBOSYL CYCLASE; AMMONIA; CD38 ANTIGEN; GLUTAMATE DEHYDROGENASE; NICOTINAMIDASE; NICOTINAMIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; NICOTINIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SIRTUIN; SIRTUIN 1; SIRTUIN 2; SIRTUIN 3;

ANALYTIC METHOD; ARTICLE; CONTINUOUS MICROPLATE ASSAY; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME METABOLISM; ENZYME REGULATION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IC 50; MAMMAL CELL; PRIORITY JOURNAL; QUANTUM YIELD; SPECTROPHOTOMETRY; STEADY STATE;

MAMMALIA;

EID: 69249206539     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.07.019     Document Type: Article

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