Taste perception: From the tongue to the testis

Molecular Human Reproduction

Volumn 19, Issue 6, 2013, Pages 349-360

  Li, Feng ^a  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

G protein coupled receptor; Male reproduction; Spermatozoa; Taste perception; Testis

Indexed keywords

G PROTEIN COUPLED RECEPTOR;

BITTER TASTE; BRAIN TISSUE; DIGESTIVE SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RESPIRATORY SYSTEM; REVIEW; SIGNAL TRANSDUCTION; SPERMATOGENESIS; TASTE; TASTE BUD; TONGUE;

G PROTEIN-COUPLED RECEPTOR; MALE REPRODUCTION; SPERMATOZOA; TASTE PERCEPTION; TESTIS;

ANIMALS; GENE EXPRESSION; HUMANS; MALE; RECEPTORS, G-PROTEIN-COUPLED; REPRODUCTION; SIGNAL TRANSDUCTION; SPERMATOGENESIS; SPERMATOZOA; TASTE; TASTE BUDS; TASTE PERCEPTION; TESTIS; TONGUE;

EID: 84880681249     PISSN: 13609947     EISSN: 14602407     Source Type: Journal    
DOI: 10.1093/molehr/gat009     Document Type: Review

References (118)

1. Abou-haila A, Tulsiani DR. Signal transduction pathways that regulate sperm capacitation and the acrosome reaction. Arch Biochem Biophys 2009;485:72-81.
2. Adler E, Hoon MA, Mueller KL, Chandrashekar J, Ryba NJ, Zuker CS. A novel family of mammalian taste receptors. Cell 2000;100:693-702.
3. Akmal KM, Dufour JM, Kim KH. Region-specific localization of retinoic acid receptor-alpha expression in the rat epididymis. Biol Reprod 1996; 54:1111-1119.
4. Bachmanov AA, Beauchamp GK. Taste receptor genes. Annu Rev Nutr 2007;27:389-414.
5. Behrens M, Meyerhof W. Mammalian bitter taste perception. Results Probl Cell Differ 2009;47:203-220.
6. Bierie B, Moses HL. Tumour microenvironment: TGFbeta: the molecular Jekyll and Hyde of cancer. Nat Rev Cancer 2006;6:506-520.
7. Breitbart H. Signaling pathways in sperm capacitation and acrosome reaction. Cell Mol Biol 2003;49:321-327.
8. Brenker C, Goodwin N, Weyand I, Kashikar ND, Naruse M, Krahling M, Muller A, Kaupp UB, Strunker T. The CatSper channel: a polymodal chemosensor in human sperm. EMBO J 2012;31:1654-1665.
9. Bufe B, Hofmann T, Krautwurst D, Raguse JD, Meyerhof W. The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides. Nat Genet 2002;32:397-401.
10. Chandrashekar J, Mueller KL, Hoon MA, Adler E, Feng L, Guo W, Zuker CS, Ryba NJ. T2Rs function as bitter taste receptors. Cell 2000;100:703-711.
11. Chandrashekar J, Hoon MA, Ryba NJ, Zuker CS. The receptors and cells for mammalian taste. Nature 2006;444:288-294.
12. Chaudhari N, Roper SD. The cell biology of taste. J Cell Biol 2010; 190:285-296.
13. Choi SH. WHO traditional medicine strategy and activities. 'Standardization with evidence-based approaches'. J Acupunct Meridian Stud 2008;1:153-154.
14. Clapp TR, Medler KF, Damak S, Margolskee RF, Kinnamon SC. Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25. BMC Biol 2006;4:7.
15. Connell CJ. An ultrastructural study of the cytoplasmic bridges between germ cells of the canine testis. Ann N Y Acad Sci 1984;438:472-475.
16. Damak S, Rong M, Yasumatsu K, Kokrashvili Z, Varadarajan V, Zou S, Jiang P, Ninomiya Y, Margolskee RF. Detection of sweet and umami taste in the absence of taste receptor T1r3. Science 2003; 301:850-853.
17. Damak S, Rong M, Yasumatsu K, Kokrashvili Z, Perez CA, Shigemura N, Yoshida R, Mosinger B Jr, Glendinning JI, Ninomiya Y et al. Trpm5 null mice respond to bitter, sweet, and umami compounds. Chem Senses 2006;31:253-264.
18. D'Cruz SC, Vaithinathan S, Jubendradass R, Mathur PP. Effects of plants and plant products on the testis. Asian J Androl 2010;12:468-479.
19. de Rooij DG. Proliferation and differentiation of spermatogonial stem cells. Reproduction 2001;121:347-354.
20. de Rooij DG, Grootegoed JA. Spermatogonial stem cells. Curr Opin Cell Biol 1998;10:694-701.
21. de Rooij DG, Mizrak SC. Deriving multipotent stem cells from mouse spermatogonial stem cells: a new tool for developmental and clinical research. Development 2008;135:2207-2213.
22. Defer N, Marinx O, Poyard M, Lienard MO, Jegou B, Hanoune J. The olfactory adenylyl cyclase type 3 is expressed in male germ cells. FEBS Lett 1998;424:216-220.
23. Deshpande DA, Wang WC, McIlmoyle EL, Robinett KS, Schillinger RM, An SS, Sham JS, Liggett SB. Bitter taste receptors on airway smooth muscle bronchodilator by localized calcium signaling and reverse obstruction. Nat Med 2010;16:1299-1304.
24. Doggrell SA. Bitter taste receptors as a target for bronchodilation. Expert Opin Ther Targets 2011;15:899-902.
25. Eddy EM. Regulation of gene expression during spermatogenesis. Semin Cell Dev Biol 1998;9:451-457.
26. Eddy EM. Male germ cell gene expression. Recent Prog Horm Res 2002; 57:103-128.
27. Egan JM, Margolskee RF. Taste cells of the gut and gastrointestinal chemosensation. Mol Interv 2008;8:78-81.
28. Elliott RA, Kapoor S, Tincello DG. Expression and distribution of the sweet taste receptor isoforms T1R2 and T1R3 in human and rat bladders. J Urol 2011;186:2455-2462.
29. Fehr J, Meyer D, Widmayer P, Borth HC, Ackermann F, Wilhelm B, Gudermann T, BoekhoffI. Expression of the G-protein alpha-subunit gustducin in mammalian spermatozoa. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 2007;193:21-34.
30. Fiehn O, Kopka J, Trethewey RN, Willmitzer L. Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. Anal Chem 2000;72:3573-3580.
31. Finger TE. Cell types and lineages in taste buds. Chem Senses 2005; 30(Suppl 1):i54-i55.
32. Finger TE, Kinnamon SC. Taste isn't just for taste buds anymore. F1000 Biol Rep 2011;3:20.
33. Fujita T. Taste cells in the gut and on the tongue. Their common, paraneuronal features. Physiol Behav 1991;49:883-885.
34. Gautier-Courteille C, Salanova M, Conti M. The olfactory adenylyl cyclase III is expressed in rat germ cells during spermiogenesis. Endocrinology 1998;139:2588-2599.
35. Goto T, Salpekar A, Monk M. Expression of a testis-specific member of the olfactory receptor gene family in human primordial germ cells. Mol Hum Reprod 2001;7:553-558.
36. Hacker K, Laskowski A, Feng L, Restrepo D, Medler K. Evidence for two populations of bitter responsive taste cells in mice. J Neurophysiol 2008; 99:1503-1514.
37. Hasegawa K, Okamura Y, Saga Y. Notch signaling in Sertoli cells regulates cyclical gene expression of Hes1 but is dispensable for mouse spermatogenesis. Mol Cell Biol 2012;32:206-215.
38. Hass N, Schwarzenbacher K, Breer H. A cluster of gustducin-expressing cells in the mouse stomach associated with two distinct populations of enteroendocrine cells. Histochem Cell Biol 2007;128:457-471.
39. Hass N, Schwarzenbacher K, Breer H. T1R3 is expressed in brush cells and ghrelin-producing cells of murine stomach. Cell Tissue Res 2010; 339:493-504.
40. Hofer D, Puschel B, Drenckhahn D. Taste receptor-like cells in the rat gut identified by expression of alpha-gustducin. Proc Natl Acad Sci USA 1996; 93:6631-6634.
41. Horio N, Yoshida R, Yasumatsu K, Yanagawa Y, Ishimaru Y, Matsunami H, Ninomiya Y. Sour taste responses in mice lacking PKD channels. PloS one 2011;6:e20007.
42. Huang AL, Chen X, Hoon MA, Chandrashekar J, Guo W, Trankner D, Ryba NJ, Zuker CS. The cells and logic for mammalian sour taste detection. Nature 2006;442:934-938.
43. Iwatsuki K, Nomura M, Shibata A, Ichikawa R, Enciso PL, Wang L, Takayanagi R, Torii K, Uneyama H. Generation and characterization of T1R2-LacZ knock-in mouse. Biochem Biophys Res Commun 2010; 402:495-499.
44. Jaiswal BS, Tur-Kaspa I, Dor J, Mashiach S, Eisenbach M. Human sperm chemotaxis: is progesterone a chemoattractant?. Biol Reprod 1999; 60:1314-1319.
45. Jang HJ, Kokrashvili Z, Theodorakis MJ, Carlson OD, Kim BJ, Zhou J, Kim HH, Xu X, Chan SL, Juhaszova M, et al. Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1. Proc Natl Acad Sci USA 2007;104:15069-15074.
46. Javelaud D, Mauviel A. Crosstalk mechanisms between the mitogen-activated protein kinase pathways and Smad signaling downstream of TGF-b: implications for carcinogenesis. Oncogene 2005;24:5742-5750.
47. Jiang P, Cui M, Zhao B, Liu Z, Snyder LA, Benard LM, Osman R, Margolskee RF, Max M. Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet taste. J Biol Chem 2005; 280:15238-15246.
48. Johnston DS, Russell LD, Friel PJ, Griswold MD. Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation. Endocrinology 2001; 142:2405-2408.
49. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 1999;47:3954-3962.
50. Kerr DS, Von Dannecker LE, Davalos M, Michaloski JS, Malnic B. Ric-8B interacts with G alpha olf and G gamma 13 and co-localizes with G alpha olf, G beta 1 and G gamma 13 in the cilia of olfactory sensory neurons. Mol Cell Neurosci 2008;38:341-348.
51. Kinnamon SC. Umami taste transduction mechanisms. Am J Clin Nutr 2009; 90:753S-755S.
52. Kiuchi S, Yamada T, Kiyokawa N, Saito T, Fujimoto J, Yasue H. Genomic structure of swine taste receptor family 1 member 3, TAS1R3, and its expression in tissues. Cytogenetic Genome Res 2006;115:51-61.
53. Klein AM, Nakagawa T, Ichikawa R, Yoshida S, Simons BD. Mouse germ line stem cells undergo rapid and stochastic turnover. Cell Stem Cell 2010;7:214-224.
54. Kokrashvili Z, Mosinger B, Margolskee RF. T1r3 and alpha-gustducin in gut regulate secretion of glucagon-like peptide-1. Ann N Y Acad Sci 2009; 1170:91-94.
55. Kulaga HM, Leitch CC, Eichers ER, Badano JL, Lesemann A, Hoskins BE, Lupski JR, Beales PL, Reed RR, Katsanis N. Loss of BBS proteins causes anosmia in humans and defects in olfactory cilia structure and function in the mouse. Nat Genet 2004;36:994-998.
56. Kumar TR, Wang Y, Lu N, Matzuk MM. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nat Genet 1997;15:201-204.
57. Lee EC, Yu D, Martinez de Velasco J, Tessarollo L, Swing DA, Court DL, Jenkins NA, Copeland NG. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 2001;73:56-65.
58. Leonard WR. Food for thought. Dietary change was a driving force in human evolution. Sci Am 2002;287:106-115.
59. Li F, Zhou M. Depletion of bitter taste transduction leads to massive spermatid loss in transgenic mice. Mol Hum Reprod 2012; 18:289-297.
60. Li X, Staszewski L, Xu H, Durick K, Zoller M, Adler E. Human receptors for sweet and umami taste. Proc Natl Acad Sci USA 2002; 99:4692-4696.
61. Li X, Li W, Wang H, Cao J, Maehashi K, Huang L, Bachmanov AA, Reed DR, Legrand-Defretin V, Beauchamp GK, et al. Pseudogenization of a sweet-receptor gene accounts for cats' indifference toward sugar. PLoS Genet 2005;1:27-35.
62. Li X, Li W, Wang H, Bayley DL, Cao J, Reed DR, Bachmanov AA, Huang L, Legrand-Defretin V, Beauchamp GK, et al. Cats lack a sweet taste receptor. J Nutr 2006;136:1932S-1934S.
63. Mace OJ, Affleck J, Patel N, Kellett GL. Sweet taste receptors in rat small intestine stimulate glucose absorption through apical GLUT2. J Physiol 2007;582:379-392.
64. Margolskee RF. The biochemistry and molecular biology of taste transduction. Curr Opin Neurobiol 1993;3:526-531.
65. Margolskee RF. Molecular mechanisms of bitter and sweet taste transduction. J Biol Chem 2002;277:1-4.
66. Margolskee RF, Dyer J, Kokrashvili Z, Salmon KS, Ilegems E, Daly K, Maillet EL, Ninomiya Y, Mosinger B, Shirazi-Beechey SP. T1R3 and gustducin in gut sense sugars to regulate expression of Na+-glucose cotransporter 1. Proc Natl Acad Sci USA 2007;104:15075-15080.
67. Masuho I, Tateyama M, Saitoh O. Characterization of bitter taste responses of intestinal STC-1 cells. Chem Senses 2005;30:281-290.
68. Max M, Shanker YG, Huang L, Rong M, Liu Z, Campagne F, Weinstein H, Damak S, Margolskee RF. Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac. Nat Genet 2001;28:58-63.
69. Meyer D, Voigt A, Widmayer P, Borth H, Huebner S, Breit A, Marschall S, de Angelis MH, Boehm U, Meyerhof W, et al. Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca(2)(+) and cAMP concentrations in spermatozoa. PloS One 2012;7:e32354.
70. Mueller KL, Hoon MA, Erlenbach I, Chandrashekar J, Zuker CS, Ryba NJ. The receptors and coding logic for bitter taste. Nature 2005; 434:225-229.
71. Nagano T. The structure of cytoplasmic bridges in dividing spermatocytes of the rooster. Anat Rec 1961;141:73-79.
72. Nakagawa Y. Function of sweet taste receptor in pancreatic beta-cells. Seikagaku 2011;83:647-651.
73. Nakagawa T, Nabeshima Y, Yoshida S. Functional identification of the actual and potential stem cell compartments in mouse spermatogenesis. Dev Cell 2007;12:195-206.
74. Nakagawa Y, Nagasawa M, Yamada S, Hara A, Mogami H, Nikolaev VO, Lohse MJ, Shigemura N, Ninomiya Y, Kojima I. Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion. PloS One 2009; 4:e5106.
75. Nakagawa T, Sharma M, Nabeshima Y, Braun RE, Yoshida S. Functional hierarchy and reversibility within the murine spermatogenic stem cell compartment. Science 2010;328:62-67.
76. Nelson G, Hoon MA, Chandrashekar J, Zhang Y, Ryba NJ, Zuker CS. Mammalian sweet taste receptors. Cell 2001;106:381-390.
77. Nelson G, Chandrashekar J, Hoon MA, Feng L, Zhao G, Ryba NJ, Zuker CS. An amino-acid taste receptor. Nature 2002;416:199-202.
78. Ota MS, Kaneko Y, Kondo K, Ogishima S, Tanaka H, Eto K, Kondo T. Combined in silico and in vivo analyses reveal role of Hes1 in taste cell differentiation. PLoS Genet 2009;5:e1000443.
79. Parmentier M, Libert F, Schurmans S, Schiffmann S, Lefort A, Eggerickx D, Ledent C, Mollereau C, Gerard C, Perret J, et al. Expression of members of the putative olfactory receptor gene family in mammalian germ cells. Nature 1992;355:453-455.
80. Patwardhan B, Warude D, Pushpangadan P, Bhatt N. Ayurveda and traditional Chinese medicine: a comparative overview. Evidence-based Complement Altern Med: eCAM 2005;2:465-473.
81. Perez CA, Huang L, Rong M, Kozak JA, Preuss AK, Zhang H, Max M, Margolskee RF. A transient receptor potential channel expressed in taste receptor cells. Nat Neurosci 2002;5:1169-1176.
82. Pritchard TC, Scott TR. Amino acids as taste stimuli. I. Neural and behavioral attributes. Brain Res 1982;253:81-92.
83. Raybould HE. Does your gut taste? sensory transduction in the gastrointestinal tract. News Physiol Sci 1998;13:275-280.
84. Ren X, Zhou L, Terwilliger R, Newton SS, de Araujo IE. Sweet taste signaling functions as a hypothalamic glucose sensor. Front Integr Neurosci 2009;3:12.
85. Riffell JA, Krug PJ, Zimmer RK. Fertilization in the sea: the chemical identity of an abalone sperm attractant. J Exp Biol 2002;205:1439-1450.
86. Rodgers S, Busch J, Peters H, Christ-Hazelhof E. Building a tree of knowledge: analysis of bitter molecules. Chem Senses 2005;30: 547-557.
87. Rozengurt E. Taste receptors in the gastrointestinal tract. I. Bitter taste receptors and alpha-gustducin in the mammalian gut. Am J Physiol Gastrointest Liver Physiol 2006;291:G171-G177.
88. Ruiz CJ, Wray K, Delay E, Margolskee RF, Kinnamon SC. Behavioral evidence for a role of alpha-gustducin in glutamate taste. Chem Senses 2003;28:573-579.
89. Shah AS, Ben-Shahar Y, Moninger TO, Kline JN, Welsh MJ. Motile cilia of human airway epithelia are chemosensory. Science 2009; 325:1131-1134.
90. Shin YJ, Park JH, Choi JS, Chun MH, Moon YW, Lee MY. Enhanced expression of the sweet taste receptors and alpha-gustducin in reactive astrocytes of the rat hippocampus following ischemic injury. Neurochem Res 2010;35:1628-1634.
91. Soranzo N, Bufe B, Sabeti PC, Wilson JF, Weale ME, Marguerie R, Meyerhof W, Goldstein DB. Positive selection on a high-sensitivity allele of the human bitter-taste receptor TAS2R16. Curr Biol: CB 2005; 15:1257-1265.
92. Spehr M, Schwane K, Riffell JA, Zimmer RK, Hatt H. Odorant receptors and olfactory-like signaling mechanisms in mammalian sperm. Mol Cell Endocrinol 2006;250:128-136.
93. Stork PJ, Schmitt JM. Crosstalk between cAMP and MAP kinase signaling in the regulation of cell proliferation. Trends Cell Biol 2002;12:258-266.
94. Strunker T, Goodwin N, Brenker C, Kashikar ND, Weyand I, Seifert R, Kaupp UB. The CatSper channel mediates progesterone-induced Ca2+ influx in human sperm. Nature 2011;471:382-386.
95. Sun F, Bahat A, Gakamsky A, Girsh E, Katz N, Giojalas LC, Tur-Kaspa I, Eisenbach M. Human sperm chemotaxis: both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants. Hum Reprod 2005;20:761-767.
96. Tang H, Brennan J, Karl J, Hamada Y, Raetzman L, Capel B. Notch signaling maintains Leydig progenitor cells in the mouse testis. Development 2008; 135:3745-3753.
97. Taniguchi K. Expression of the sweet receptor protein, T1R3, in the human liver and pancreas. J Vet Med Sci 2004;66:1311-1314.
98. Tizzano M, Dvoryanchikov G, Barrows JK, Kim S, Chaudhari N, Finger TE. Expression of Galpha14 in sweet-transducing taste cells of the posterior tongue. BMC Neurosci 2008;9:110.
99. Tizzano M, Gulbransen BD, Vandenbeuch A, Clapp TR, Herman JP, Sibhatu HM, Churchill ME, Silver WL, Kinnamon SC, Finger TE. Nasal chemosensory cells use bitter taste signaling to detect irritants and bacterial signals. Proc Natl Acad Sci USA 2010;107:3210-3215.
100. Tizzano M, Cristofoletti M, Sbarbati A, Finger TE. Expression of taste receptors in solitary chemosensory cells of rodent airways. BMC Pulm Med 2011;11:3.
101. TordoffMG, Shao H, Alarcon LK, Margolskee RF, Mosinger B, Bachmanov AA, Reed DR, McCaughey S. Involvement of T1R3 in calcium-magnesium taste. Physiol Genomics 2008;34:338-348.
102. TordoffMG, Alarcon LK, Valmeki S, Jiang P. T1R3: a human calcium taste receptor. Sci Rep 2012;2:496.
103. Treesukosol Y, Smith KR, Spector AC. Behavioral evidence for a glucose polymer taste receptor that is independent of the T1R2+3 heterodimer in a mouse model. J Neurosci 2011a;31:13527-13534.
104. Treesukosol Y, Smith KR, Spector AC. The functional role of the T1R family of receptors in sweet taste and feeding. Physiol Behav 2011b; 105:14-26.
105. Trivedi BP. Neuroscience: hardwired for taste. Nature 2012;486:S7-S9.
106. Vanderhaeghen P, Schurmans S, Vassart G, Parmentier M. Specific repertoire of olfactory receptor genes in the male germ cells of several mammalian species. Genomics 1997;39:239-246.
107. Voigt A, Hubner S, Lossow K, Hermans-Borgmeyer I, Boehm U, Meyerhof W. Genetic labeling of tas1r1 and tas2r131 taste receptor cells in mice. Chem Senses 2012;37:897-911.
108. Wettschureck N, Offermanns S. Mammalian G proteins and their cell type specific functions. Physiol Rev 2005;85:1159-1204.
109. Wiener A, Shudler M, Levit A, Niv MY. BitterDB: a database of bitter compounds. Nucleic Acids Res 2012;40:D413-D419.
110. Wong GT, Gannon KS, Margolskee RF. Transduction of bitter and sweet taste by gustducin. Nature 1996;381:796-800.
111. Wong GT, Ruiz-Avila L, Margolskee RF. Directing gene expression to gustducin-positive taste receptor cells. J Neurosci 1999;19:5802-5809.
112. Wooding S, Kim UK, Bamshad MJ, Larsen J, Jorde LB, Drayna D. Natural selection and molecular evolution in PTC, a bitter-taste receptor gene. Am J Hum Genet 2004;74:637-646.
113. Wu SV, Rozengurt N, Yang M, Young SH, Sinnett-Smith J, Rozengurt E. Expression of bitter taste receptors of the T2R family in the gastrointestinal tract and enteroendocrine STC-1 cells. Proc Natl Acad Sci USA 2002;99:2392-2397.
114. Xu J, Cao J, Iguchi N, Riethmacher D, Huang L. Functional characterization of bitter-taste receptors expressed in mammalian testis. Mol Hum Reprod 2013;19:17-28.
115. Zancanaro C, Caretta CM, Merigo F, Cavaggioni A, Osculati F. alpha-Gustducin expression in the vomeronasal organ of the mouse. Eur J Neurosci 1999;11:4473-4475.
116. Zhang Y, Hoon MA, Chandrashekar J, Mueller KL, Cook B, WuD, Zuker CS, Ryba NJ. Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. Cell 2003;112:293-301.
117. Zhang Z, Zhao Z, Margolskee R, Liman E. The transduction channel TRPM5 is gated by intracellular calcium in taste cells. J Neurosci 2007; 27:5777-5786.
118. Zhao GQ, Zhang Y, Hoon MA, Chandrashekar J, Erlenbach I, Ryba NJ, Zuker CS. The receptors for mammalian sweet and umami taste. Cell 2003;115:255-266.