A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA

Genomics

Volumn 73, Issue 1, 2001, Pages 56-65

  Lee, E Chiang ^a   Yu, Daiguan ^a   Martinez De Velasco, J ^a   Tessarollo, Lino ^a   Swing, Deborah A ^a   Court, Donald L ^a   Jenkins, Nancy A ^a   Copeland, Neal G ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYDEOXYRIBONUCLEOTIDE SYNTHASE;

ADAPTATION; ANIMAL CELL; BACTERIAL ARTIFICIAL CHROMOSOME; CELL MATURATION; CELL VIABILITY; DNA FLANKING REGION; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE DELETION; GENE TARGETING; GENETIC ENGINEERING; GENETIC RECOMBINATION; MOLECULAR CLONING; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROPHAGE; REVIEW; STRAIN DIFFERENCE; TARGET CELL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MUS MUSCULUS;

EID: 0035308590     PISSN: 08887543     EISSN: None     Source Type: Journal    
DOI: 10.1006/geno.2000.6451     Document Type: Article

References (13)

1. Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6
2. Functional identification of the mouse circadian clock gene by transgenic BAC rescue
3. Targeted mutagenesis of the endogenous mouse Mis gene promoter: In vivo definition of genetic pathways of vertebrate sexual development
4. Improved properties of FLP recombinase evolved by cycling mutagenesis
5. Cosmid DNA packaging in vivo
6. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: Application to open reading frame characterization
7. Neuron specific enolase, a clinically useful marker for neurons and neuroendocrine cells
8. Rapid modification of bacterial artificial chromosomes by ET-recombination
9. Efficient and precise engineering of a 200 kb beta-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system
10. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector
11. Generalized lacZ expression with the ROSA26 Cre reporter strain
12. An efficient recombination system for chromosome engineering in Escherichia coli
13. A new logic for DNA engineering using recombination in Escherichia coli