Cell cycle regulation by the NEK family of protein kinases

Journal of Cell Science

Volumn 125, Issue 19, 2012, Pages 4423-4433

  Fry, Andrew M ^a   O'Regan, Laura ^a   Sabir, Sarah R ^a   Bayliss, Richard ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Centrosome; Cilia; DNA damage response; Microtubule; Mitosis; Mitotic spindle; NEK; NIMA

Indexed keywords

NEVER IN MITOSIS A RELATED KINASE; PROTEIN SERINE THREONINE KINASE; UNCLASSIFIED DRUG;

ALLOSTERISM; ANTINEOPLASTIC ACTIVITY; ARTICLE; ASPERGILLUS NIDULANS; BILE DUCT CARCINOMA; BREAST CANCER; CATALYSIS; CELL CYCLE ARREST; CELL CYCLE PROGRESSION; CELL CYCLE REGULATION; CELL NUCLEUS MEMBRANE; CELL STRUCTURE; CHROMATIN CONDENSATION; CILIARY MOTILITY; COLORECTAL CANCER; CYTOKINESIS; DNA DAMAGE; DRUG TARGETING; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME LOCALIZATION; EUKARYOTIC FLAGELLUM; HUMAN; INTERPHASE; KINETOSOME; M PHASE CELL CYCLE CHECKPOINT; MICROTUBULE; MITOSIS; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SIGNAL TRANSDUCTION;

ALLOSTERIC REGULATION; ANIMALS; ANTINEOPLASTIC AGENTS; CELL CYCLE CHECKPOINTS; CELL CYCLE PROTEINS; HUMANS; MITOSIS; MULTIGENE FAMILY; PROTEIN-SERINE-THREONINE KINASES;

EMERICELLA NIDULANS; EUKARYOTA; FUNGI;

EID: 84872171489     PISSN: 00219533     EISSN: 14779137     Source Type: Journal    
DOI: 10.1242/jcs.111195     Document Type: Article

References (108)

1. Alexander, J., Lim, D., Joughin, B. A., Hegemann, B., Hutchins, J. R., Ehrenberger, T., Ivins, F., Sessa, F., Hudecz, O., Nigg, E. A. et al. (2011). Spatial exclusivity combined with positive and negative selection of phosphorylation motifs is the basis for context-dependent mitotic signaling. Sci. Signal. 4, ra42.
2. Andersen, J. S., Wilkinson, C. J., Mayor, T., Mortensen, P., Nigg, E. A. and Mann, M. (2003). Proteomic characterization of the human centrosome by protein correlation profiling. Nature 426, 570-574.
3. Bahe, S., Stierhof, Y. D., Wilkinson, C. J., Leiss, F. and Nigg, E. A. (2005). Rootletin forms centriole-associated filaments and functions in centrosome cohesion. J. Cell Biol. 171, 27-33.
4. Bahmanyar, S., Kaplan, D. D., Deluca, J. G., Giddings, T. H., Jr, O'Toole, E. T., Winey, M., Salmon, E. D., Casey, P. J., Nelson, W. J. and Barth, A. I. (2008). beta-Catenin is a Nek2 substrate involved in centrosome separation. Genes Dev. 22, 91-105.
5. Belham, C., Roig, J., Caldwell, J. A., Aoyama, Y., Kemp, B. E., Comb, M. and Avruch, J. (2003). A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases. J. Biol. Chem. 278, 34897-34909.
6. Bertran, M. T., Sdelci, S., Regué, L., Avruch, J., Caelles, C. and Roig, J. (2011). Nek9 is a Plk1-activated kinase that controls early centrosome separation through Nek6/7 and Eg5. EMBO J. 30, 2634-2647.
7. Bettencourt-Dias, M., Hildebrandt, F., Pellman, D., Woods, G. and Godinho, S. A. (2011). Centrosomes and cilia in human disease. Trends Genet. 27, 307-315.
8. Blangy, A., Lane, H. A., d'Hérin, P., Harper, M., Kress, M. and Nigg, E. A. (1995). Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesinrelated motor essential for bipolar spindle formation in vivo. Cell 83, 1159-1169.
9. Bradley, B. A. and Quarmby, L. M. (2005). A NIMA-related kinase, Cnk2p, regulates both flagellar length and cell size in Chlamydomonas. J. Cell Sci. 118, 3317-3326.
10. Chang, J., Baloh, R. H. and Milbrandt, J. (2009). The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons. J. Cell Sci. 122, 2274-2282.
11. Chen, Y., Chen, P. L., Chen, C. F., Jiang, X. and Riley, D. J. (2008). Never-in-mitosis related kinase 1 functions in DNA damage response and checkpoint control. Cell Cycle 7, 3194-3201.
12. Chen, Y., Chen, C. F., Riley, D. J. and Chen, P. L. (2011). Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR. Cell Cycle 10, 655-663.
13. Coene, K. L., Mans, D. A., Boldt, K., Gloeckner, C. J., van Reeuwijk, J., Bolat, E., Roosing, S., Letteboer, S. J., Peters, T. A., Cremers, F. P. et al. (2011). The ciliopathy-associated protein homologs RPGRIP1 and RPGRIP1L are linked to cilium integrity through interaction with Nek4 serine/threonine kinase. Hum. Mol. Genet. 20, 3592-3605.
14. Croasdale, R., Ivins, F. J., Muskett, F., Daviter, T., Scott, D. J., Hardy, T., Smerdon, S. J., Fry, A. M. and Pfuhl, M. (2011). An undecided coiled coil: the leucine zipper of Nek2 kinase exhibits atypical conformational exchange dynamics. J. Biol. Chem. 286, 27537-27547.
15. De Souza, C. P., Osmani, A. H., Wu, L. P., Spotts, J. L. and Osmani, S. A. (2000). Mitotic histone H3 phosphorylation by the NIMA kinase in Aspergillus nidulans. Cell 102, 293-302.
16. Di Agostino, S., Fedele, M., Chieffi, P., Fusco, A., Rossi, P., Geremia, R. and Sette, C. (2004). Phosphorylation of high-mobility group protein A2 by Nek2 kinase during the first meiotic division in mouse spermatocytes. Mol. Biol. Cell 15, 1224-1232.
17. Du, J., Cai, X., Yao, J., Ding, X., Wu, Q., Pei, S., Jiang, K., Zhang, Y., Wang, W., Shi, Y. et al. (2008). The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability. Oncogene 27, 4107-4114.
18. Faragher, A. J. and Fry, A. M. (2003). Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles. Mol. Biol. Cell 14, 2876-2889.
19. Fletcher, L., Cerniglia, G. J., Nigg, E. A., Yend, T. J. and Muschel, R. J. (2004). Inhibition of centrosome separation after DNA damage: a role for Nek2. Radiat. Res. 162, 128-135.
20. Fletcher, L., Cerniglia, G. J., Yen, T. J. and Muschel, R. J. (2005). Live cell imaging reveals distinct roles in cell cycle regulation for Nek2A and Nek2B. Biochim. Biophys. Acta 1744, 89-92.
21. Fry, A. M., Mayor, T., Meraldi, P., Stierhof, Y. D., Tanaka, K. and Nigg, E. A. (1998a). C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. J. Cell Biol. 141, 1563-1574.
22. Fry, A. M., Meraldi, P. and Nigg, E. A. (1998b). A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators. EMBO J. 17, 470-481.
23. Fry, A. M., Arnaud, L. and Nigg, E. A. (1999). Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif. J. Biol. Chem. 274, 16304-16310.
24. Gascoigne, K. E. and Taylor, S. S. (2008). Cancer cells display profound intra- and interline variation following prolonged exposure to antimitotic drugs. Cancer Cell 14, 111-122.
25. Goshima, G. and Kimura, A. (2010). New look inside the spindle: microtubuledependent microtubule generation within the spindle. Curr. Opin. Cell Biol. 22, 44-49.
26. Grallert, A. and Hagan, I. M. (2002). Schizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB. EMBO J. 21, 3096-3107.
27. Grallert, A., Krapp, A., Bagley, S., Simanis, V. and Hagan, I. M. (2004). Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity. Genes Dev. 18, 1007-1021.
28. Graser, S., Stierhof, Y. D. and Nigg, E. A. (2007). Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion. J. Cell Sci. 120, 4321-4331.
29. Hames, R. S., Wattam, S. L., Yamano, H., Bacchieri, R. and Fry, A. M. (2001). APC/ C-mediated destruction of the centrosomal kinase Nek2A occurs in early mitosis and depends upon a cyclin A-type D-box. EMBO J. 20, 7117-7127.
30. Hayes, M. J., Kimata, Y., Wattam, S. L., Lindon, C., Mao, G., Yamano, H. and Fry, A. M. (2006). Early mitotic degradation of Nek2A depends on Cdc20-independent interaction with the APC/C. Nat. Cell Biol. 8, 607-614.
31. Hayward, D. G., Clarke, R. B., Faragher, A. J., Pillai, M. R., Hagan, I. M. and Fry, A. M. (2004). The centrosomal kinase Nek2 displays elevated levels of protein expression in human breast cancer. Cancer Res. 64, 7370-7376.
32. Helps, N. R., Luo, X., Barker, H. M. and Cohen, P. T. (2000). NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1. Biochem. J. 349, 509-518.
33. Henise, J. C. and Taunton, J. (2011). Irreversible Nek2 kinase inhibitors with cellular activity. J. Med. Chem. 54, 4133-4146.
34. Holland, P. M., Milne, A., Garka, K., Johnson, R. S., Willis, C., Sims, J. E., Rauch, C. T., Bird, T. A. and Virca, G. D. (2002). Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2. J. Biol. Chem. 277, 16229-16240.
35. Innocenti, P., Cheung, K. M., Solanki, S., Mas-Droux, C., Rowan, F., Yeoh, S., Boxall, K., Westlake, M., Pickard, L., Hardy, T. et al. (2012). Design of potent and selective hybrid inhibitors of the mitotic kinase Nek2: structure-activity relationship, structural biology, and cellular activity. J. Med. Chem. 55, 3228-3241.
36. Jackson, J. R., Patrick, D. R., Dar, M. M. and Huang, P. S. (2007). Targeted antimitotic therapies: can we improve on tubulin agents? Nat. Rev. Cancer 7, 107-117.
37. Jee, H. J., Kim, A. J., Song, N., Kim, H. J., Kim, M., Koh, H. and Yun, J. (2010). Nek6 overexpression antagonizes p53-induced senescence in human cancer cells. Cell Cycle 9, 4703-4710.
38. Jeon, Y. J., Lee, K. Y., Cho, Y. Y., Pugliese, A., Kim, H. G., Jeong, C. H., Bode, A. M. and Dong, Z. (2010). Role of NEK6 in tumor promoter-induced transformation in JB6 C141 mouse skin epidermal cells. J. Biol. Chem. 285, 28126-28133.
39. Jeong, Y., Lee, J., Kim, K., Yoo, J. C. and Rhee, K. (2007). Characterization of NIP2/ centrobin, a novel substrate of Nek2, and its potential role in microtubule stabilization. J. Cell Sci. 120, 2106-2116.
40. Johnson, L. N., Noble, M. E. and Owen, D. J. (1996). Active and inactive protein kinases: structural basis for regulation. Cell 85, 149-158.
41. Jordan, M. A. and Wilson, L. (2004). Microtubules as a target for anticancer drugs. Nat. Rev. Cancer 4, 253-265.
42. Kim, S., Lee, K. and Rhee, K. (2007). NEK7 is a centrosomal kinase critical for microtubule nucleation. Biochem. Biophys. Res. Commun. 360, 56-62.
43. Kim, S., Kim, S. and Rhee, K. (2011). NEK7 is essential for centriole duplication and centrosomal accumulation of pericentriolar material proteins in interphase cells. J. Cell Sci. 124, 3760-3770.
44. Kokuryo, T., Senga, T., Yokoyama, Y., Nagino, M., Nimura, Y. and Hamaguchi, M. (2007). Nek2 as an effective target for inhibition of tumorigenic growth and peritoneal dissemination of cholangiocarcinoma. Cancer Res. 67, 9637-9642.
45. Komlodi-Pasztor, E., Sackett, D., Wilkerson, J. and Fojo, T. (2011). Mitosis is not a key target of microtubule agents in patient tumors. Nat. Rev. Clin. Oncol. 8, 244-250.
46. Krien, M. J., Bugg, S. J., Palatsides, M., Asouline, G., Morimyo, M. and O'Connell, M. J. (1998). A NIMA homologue promotes chromatin condensation in fission yeast. J. Cell Sci. 111, 967-976.
47. Krien, M. J., West, R. R., John, U. P., Koniaras, K., McIntosh, J. R. and O'Connell, M. J. (2002). The fission yeast NIMA kinase Fin1p is required for spindle function and nuclear envelope integrity. EMBO J. 21, 1713-1722.
48. Laurell, E., Beck, K., Krupina, K., Theerthagiri, G., Bodenmiller, B., Horvath, P., Aebersold, R., Antonin, W. and Kutay, U. (2011). Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry. Cell 144, 539-550.
49. Lee, M. Y., Kim, H. J., Kim, M. A., Jee, H. J., Kim, A. J., Bae, Y. S., Park, J. I., Chung, J. H. and Yun, J. (2008). Nek6 is involved in G2/M phase cell cycle arrest through DNA damage-induced phosphorylation. Cell Cycle 7, 2705-2709.
50. Liu, S., Lu, W., Obara, T., Kuida, S., Lehoczky, J., Dewar, K., Drummond, I. A. and Beier, D. R. (2002). A defect in a novel Nek-family kinase causes cystic kidney disease in the mouse and in zebrafish. Development 129, 5839-5846.
51. Lizcano, J. M., Deak, M., Morrice, N., Kieloch, A., Hastie, C. J., Dong, L., Schutkowski, M., Reimer, U. and Alessi, D. R. (2002). Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo. J. Biol. Chem. 277, 27839-27849.
52. Loncarek, J., Hergert, P., Magidson, V. and Khodjakov, A. (2008). Control of daughter centriole formation by the pericentriolar material. Nat. Cell Biol. 10, 322-328.
53. Lou, Y., Yao, J., Zereshki, A., Dou, Z., Ahmed, K., Wang, H., Hu, J., Wang, Y. and Yao, X. (2004). NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling. J. Biol. Chem. 279, 20049-20057.
54. Lu, K. P. and Hunter, T. (1995). Evidence for a NIMA-like mitotic pathway in vertebrate cells. Cell 81, 413-424.
55. Lu, K. P., Kemp, B. E. and Means, A. R. (1994). Identification of substrate specificity determinants for the cell cycle-regulated NIMA protein kinase. J. Biol. Chem. 269, 6603-6607.
56. Mahjoub, M. R., Montpetit, B., Zhao, L., Finst, R. J., Goh, B., Kim, A. C. and Quarmby, L. M. (2002). The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. J. Cell Sci. 115, 1759-1768.
57. Mardin, B. R., Lange, C., Baxter, J. E., Hardy, T., Scholz, S. R., Fry, A. M. and Schiebel, E. (2010). Components of the Hippo pathway cooperate with Nek2 kinase to regulate centrosome disjunction. Nat. Cell Biol. 12, 1166-1176.
58. Mardin, B. R., Agircan, F. G., Lange, C. and Schiebel, E. (2011). Plk1 controls the Nek2A-PP1γ antagonism in centrosome disjunction. Curr. Biol. 21, 1145-1151.
59. Melixetian, M., Klein, D. K., Sørensen, C. S. and Helin, K. (2009). NEK11 regulates CDC25A degradation and the IR-induced G2/M checkpoint. Nat. Cell Biol. 11, 1247-1253.
60. Miller, S. L., Antico, G., Raghunath, P. N., Tomaszewski, J. E. and Clevenger, C. V. (2007). Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene 26, 4668-4678.
61. Moniz, L. S. and Stambolic, V. (2011). Nek10 mediates G2/M cell cycle arrest and MEK autoactivation in response to UV irradiation. Mol. Cell. Biol. 31, 30-42.
62. Moniz, L., Dutt, P., Haider, N. and Stambolic, V. (2011). Nek family of kinases in cell cycle, checkpoint control and cancer. Cell Div. 6, 18.
63. Nassirpour, R., Shao, L., Flanagan, P., Abrams, T., Jallal, B., Smeal, T. and Yin, M. J. (2010). Nek6 mediates human cancer cell transformation and is a potential cancer therapeutic target. Mol. Cancer Res. 8, 717-728.
64. Noguchi, K., Fukazawa, H., Murakami, Y. and Uehara, Y. (2002). Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses. J. Biol. Chem. 277, 39655-39665.
65. Noguchi, K., Fukazawa, H., Murakami, Y. and Uehara, Y. (2004). Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells. J. Biol. Chem. 279, 32716-32727.
66. O'Connell, M. J., Norbury, C. and Nurse, P. (1994). Premature chromatin condensation upon accumulation of NIMA. EMBO J. 13, 4926-4937.
67. O'Connell, M. J., Krien, M. J. and Hunter, T. (2003). Never say never. The NIMArelated protein kinases in mitotic control. Trends Cell Biol. 13, 221-228.
68. O'Regan, L., Blot, J. and Fry, A. M. (2007). Mitotic regulation by NIMA-related kinases. Cell Div. 2, 25.
69. O'Regan, L. and Fry, A. M. (2009). The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis. Mol. Cell. Biol. 29, 3975-3990.
70. Oakley, B. R. and Morris, N. R. (1983). A mutation in Aspergillus nidulans that blocks the transition from interphase to prophase. J. Cell Biol. 96, 1155-1158.
71. Osmani, S. A., Pu, R. T. and Morris, N. R. (1988). Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase. Cell 53, 237-244.
72. Osmani, A. H., McGuire, S. L. and Osmani, S. A. (1991). Parallel activation of the NIMA and p34cdc2 cell cycle-regulated protein kinases is required to initiate mitosis in A. nidulans. Cell 67, 283-291.
73. Otto, E. A., Trapp, M. L., Schultheiss, U. T., Helou, J., Quarmby, L. M. and Hildebrandt, F. (2008). NEK8 mutations affect ciliary and centrosomal localization and may cause nephronophthisis. J. Am. Soc. Nephrol. 19, 587-592.
74. Parker, J. D., Bradley, B. A., Mooers, A. O. and Quarmby, L. M. (2007). Phylogenetic analysis of the Neks reveals early diversification of ciliary-cell cycle kinases. PLoS ONE 2, e1076.
75. Pelegrini, A. L., Moura, D. J., Brenner, B. L., Ledur, P. F., Maques, G. P., Henriques, J. A., Saffi, J. and Lenz, G. (2010). Nek1 silencing slows down DNA repair and blocks DNA damage-induced cell cycle arrest. Mutagenesis 25, 447-454.
76. Polci, R., Peng, A., Chen, P. L., Riley, D. J. and Chen, Y. (2004). NIMA-related protein kinase 1 is involved early in the ionizing radiation-induced DNA damage response. Cancer Res. 64, 8800-8803.
77. Prigent, C., Glover, D. M. and Giet, R. (2005). Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failure. Exp. Cell Res. 303, 1-13.
78. Pu, R. T. and Osmani, S. A. (1995). Mitotic destruction of the cell cycle regulated NIMA protein kinase of Aspergillus nidulans is required for mitotic exit. EMBO J. 14, 995-1003.
79. Pugacheva, E. N. and Golemis, E. A. (2005). The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome. Nat. Cell Biol. 7, 937-946.
80. Pugacheva, E. N., Jablonski, S. A., Hartman, T. R., Henske, E. P. and Golemis, E. A. (2007). HEF1-dependent Aurora A activation induces disassembly of the primary cilium. Cell 129, 1351-1363.
81. Quarmby, L. M. and Mahjoub, M. R. (2005). Caught Nek-ing: cilia and centrioles. J. Cell Sci. 118, 5161-5169.
82. Rapley, J., Nicola' s, M., Groen, A., Regué, L., Bertran, M. T., Caelles, C., Avruch, J. and Roig, J. (2008). The NIMA-family kinase Nek6 phosphorylates the kinesin Eg5 at a novel site necessary for mitotic spindle formation. J. Cell Sci. 121, 3912-3921.
83. Rellos, P., Ivins, F. J., Baxter, J. E., Pike, A., Nott, T. J., Parkinson, D. M., Das, S., Howell, S., Fedorov, O., Shen, Q. Y. et al. (2007). Structure and regulation of the human Nek2 centrosomal kinase. J. Biol. Chem. 282, 6833-6842.
84. Richards, M. W., O'Regan, L., Mas-Droux, C., Blot, J. M., Cheung, J., Hoelder, S., Fry, A. M. and Bayliss, R. (2009). An autoinhibitory tyrosine motif in the cell-cycleregulated Nek7 kinase is released through binding of Nek9. Mol. Cell 36, 560-570.
85. Roig, J., Mikhailov, A., Belham, C. and Avruch, J. (2002). Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression. Genes Dev. 16, 1640-1658.
86. Roig, J., Groen, A., Caldwell, J. and Avruch, J. (2005). Active Nercc1 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly. Mol. Biol. Cell 16, 4827-4840.
87. Salem, H., Rachmin, I., Yissachar, N., Cohen, S., Amiel, A., Haffner, R., Lavi, L. and Motro, B. (2010). Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy. Oncogene 29, 4046-4057.
88. Sawin, K. E. and Mitchison, T. J. (1995). Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle. Proc. Natl. Acad. Sci. USA 92, 4289-4293.
89. Sdelci, S., Bertran, M. T. and Roig, J. (2011). Nek9, Nek6, Nek7 and the separation of centrosomes. Cell Cycle 10, 3816-3817.
90. Sdelci, S., Schütz, M., Pinyol, R., Bertran, M. T., Regué, L., Caelles, C., Vernos, I. and Roig, J. (2012). Nek9 phosphorylation of NEDD1/GCP-WD contributes to Plk1 control of γ-tubulin recruitment to the mitotic centrosome. Curr. Biol. 22, 1516-1523.
91. Spalluto, C., Wilson, D. I. and Hearn, T. (2012). Nek2 localises to the distal portion of the mother centriole/basal body and is required for timely cilium disassembly at the G2/M transition. Eur. J. Cell Biol. 91, 675-686.
92. Suzuki, K., Kokuryo, T., Senga, T., Yokoyama, Y., Nagino, M. and Hamaguchi, M. (2010). Novel combination treatment for colorectal cancer using Nek2 siRNA and cisplatin. Cancer Sci. 101, 1163-1169.
93. Thiel, C., Kessler, K., Giessl, A., Dimmler, A., Shalev, S. A., von der Haar, S., Zenker, M., Zahnleiter, D., Stss, H., Beinder, E. et al. (2011). NEK1 mutations cause short-rib polydactyly syndrome type majewski. Am. J. Hum. Genet. 88, 106-114.
94. Tsunoda, N., Kokuryo, T., Oda, K., Senga, T., Yokoyama, Y., Nagino, M., Nimura, Y. and Hamaguchi, M. (2009). Nek2 as a novel molecular target for the treatment of breast carcinoma. Cancer Sci. 100, 111-116.
95. Upadhya, P., Birkenmeier, E. H., Birkenmeier, C. S. and Barker, J. E. (2000). Mutations in a NIMA-related kinase gene, Nek1, cause pleiotropic effects including a progressive polycystic kidney disease in mice. Proc. Natl. Acad. Sci. USA 97, 217-221.
96. Vaz Meirelles, G., Ferreira Lanza, D. C., da Silva, J. C., Santana Bernachi, J., Paes Leme, A. F. and Kobarg, J. (2010). Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome. J. Proteome Res. 9, 6298-6316.
97. Vogler, C., Homan, S., Pung, A., Thorpe, C., Barker, J., Birkenmeier, E. H. and Upadhya, P. (1999). Clinical and pathologic findings in two new allelic murine models of polycystic kidney disease. J. Am. Soc. Nephrol. 10, 2534-2539.
98. Wei, R., Ngo, B., Wu, G. and Lee, W. H. (2011). Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint. Mol. Biol. Cell 22, 3584-3594.
99. Westwood, I., Cheary, D. M., Baxter, J. E., Richards, M. W., van Montfort, R. L., Fry, A. M. and Bayliss, R. (2009). Insights into the conformational variability and regulation of human Nek2 kinase. J. Mol. Biol. 386, 476-485.
100. Whelligan, D. K., Solanki, S., Taylor, D., Thomson, D. W., Cheung, K.-M., Boxall, K., Mas-Droux, C., Barillari, C., Burns, S., Grummitt, C. G. et al. (2010). Aminopyrazine inhibitors binding to an unusual inactive conformation of the mitotic kinase Nek2: SAR and structural characterization. J. Med. Chem. 53, 7682-7698.
101. Wloga, D., Camba, A., Rogowski, K., Manning, G., Jerka-Dziadosz, M. and Gaertig, J. (2006). Members of the NIMA-related kinase family promote disassembly of cilia by multiple mechanisms. Mol. Biol. Cell 17, 2799-2810.
102. Wu, G., Qiu, X. L., Zhou, L., Zhu, J., Chamberlin, R., Lau, J., Chen, P. L. and Lee, W. H. (2008). Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal. Cancer Res. 68, 8393-8399.
103. Wu, L., Osmani, S. A. and Mirabito, P. M. (1998). A role for NIMA in the nuclear localization of cyclin B in Aspergillus nidulans. J. Cell Biol. 141, 1575-1587.
104. Wu, W., Baxter, J. E., Wattam, S. L., Hayward, D. G., Fardilha, M., Knebel, A., Ford, E. M., da Cruze Silva, E. F. and Fry, A. M. (2007). Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase. J. Biol. Chem. 282, 26431-26440.
105. Yang, J., Adamian, M. and Li, T. (2006). Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells. Mol. Biol. Cell 17, 1033-1040.
106. Yim, H., Sung, C. K., You, J., Tian, Y. and Benjamin, T. (2011). Nek1 and TAZ interact to maintain normal levels of polycystin 2. J. Am. Soc. Nephrol. 22, 832-837.
107. Yissachar, N., Salem, H., Tennenbaum, T. and Motro, B. (2006). Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression. FEBS Lett. 580, 6489-6495.
108. Zalli, D., Bayliss, R. and Fry, A. M. (2012). The Nek8 protein kinase, mutated in the human cystic kidney disease nephronophthisis, is both activated and degraded during ciliogenesis. Hum. Mol. Genet. 21, 1155-1171.