Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles

Molecular Biology of the Cell

Volumn 14, Issue 7, 2003, Pages 2876-2889

  Faragher, Alison J ^a   Fry, Andrew M ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CYCLE PROTEIN; DNA; NEK2A KINASE; PHOSPHOTRANSFERASE; PROTEIN C NAP1; TETRACYCLINE; UNCLASSIFIED DRUG;

ARTICLE; CELL ADHESION; CELL CYCLE; CELL CYCLE G2 PHASE; CELL POPULATION; CENTRIOLE; CENTROSOME; CHROMOSOME SEGREGATION; CONTROLLED STUDY; CYTOKINESIS; ENZYME ACTIVATION; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; INTERPHASE; MITOSIS; MITOSIS SPINDLE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SPINDLE CELL;

AUTOANTIGENS; CELL CYCLE PROTEINS; CELLS, CULTURED; CENTRIOLES; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE EXPRESSION REGULATION; HUMANS; MITOSIS; MITOTIC SPINDLE APPARATUS; MODELS, MOLECULAR; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS;

EID: 0037769884     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E03-02-0108     Document Type: Article

References (45)

1. cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 83, 1159-1169.
2. Bobinnec, Y., Moudjou, M., Fouquet, J.P., Desbruyeres, E., Edde, B., and Bornens, M. (1998). Glutamylation of centriole and cytoplasmic tubulin in proliferating non-neuronal cells. Cell Motil. Cytoskeleton 39, 223-232.
3. Bornens, M., and Moudjou, M. (1999). Studying the composition and function of centrosomes in vertebrates. Methods Cell Biol. 61, 13-34.
4. Bornens, M., Paintrand, M., Berges, J., Marty, M.C., and Karsenti, E. (1987). Structural and chemicaI characterization of isolated centrosomes. Cell Motil. Cytoskeleton 8, 238-249.
5. Chevrier, V., Piel, M., Collomb, N., Saoudi, Y., Frank, R., Paintrand, M., Narumiya, S., Bornens, M., and Job, D. (2002). The Rho-associated protein kinase p160ROCK is required for centrosome positioning. J. Cell Biol. 157, 807-817.
6. Doxsey, S. (2001). Re-evaluating centrosome function. Nat. Rev Mol. Cell. Biol. 2, 688-698.
7. Eto, M., Elliot, E., Prickett, T.D., and Brautigan, D.L. (2002). Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation. J. Biol. Chem. 277, 44013-44020.
8. Fry, A.M. (2002). The Nek2 protein kinase: a novel regulator of centrosome structure. Oncogene 21, 6184-6194.
9. Fry, A.M., Arnaud, L., and Nigg, E.A. (1999). Activity of the human centrosomal kinase, Nek2, depends upon an unusual leucine zipper dimerization motif. J. Biol. Chem. 274, 16304-16310.
10. Fry, A.M., Descombes, P., Twomey, C., Bacchieri, R., and Nigg, E.A. (2000b). The NIMA-related kinase X-Nek2B is required for efficient assembly of the zygotic centrosome in Xenopus laevis. J. Cell Sci. 113, 1973-1984.
11. Fry, A.M., and Faragher, A.J. (2001). Identification of centrosome kinases. Methods Cell Biol. 67, 305-323.
12. Fry, A.M., Mayor, T., Meraldi, P., Stierhof, Y.-D., Tanaka, K., and Nigg, E.A. (1998b). C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. J. Cell Biol. 141, 1563-1574.
13. Fry, A.M., Meraldi, P., and Nigg, E.A. (1998a). A centrosomal function for the human Nek2 protein kinase, a member of the NIMA-family of cell cycle regulators. EMBO J. 17, 470-481.
14. Fry, A.M., Schultz, S.J., Bartek, J., and Nigg, E.A. (1995). Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans. J. Biol. Chem. 270, 12899-12905.
15. Golsteyn, R.M., Mundt, K.E., Fry, A.M., and Nigg, E.A. (1995). Cell cycle regulation of the activity and subcellular localization of PLK1, a human protein kinase implicated in mitotic spindle function. J. Cell Biol. 129, 1617-1628.
16. Hames, R.S., and Fry, A.M. (2002). Alternative splice variants of the human centrosomal kinase Nek2 exhibit distinct patterns of expression in mitosis. Biochem. J. 361, 77-85.
17. Hames, R.S., Wattam, S.L., Yamano, H., Bacchieri, R., and Fry, A.M. (2001). APC/C-mediated destruction of the centrosomal kinase Nek2A occurs in early mitosis and depends upon a cyclin A-type D-box. EMBO J. 20, 7117-7127.
18. Helps, N.R., Luo, X., Barker, H.M., and Cohen, P.T.W. (2000). NIMA-related kinase 2 (Nek2), a cell cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1. Biochem. J. 349, 509-518.
19. Hinchcliffe, E.H., and Sluder, G. (2001). "It takes two to tango": understanding how centrosome duplication is regulated throughout the cell cycle. Genes Dev. 15, 1167-1181.
20. Kapoor, T.M., Mayer, T.U., Coughlin, M.L., and Mitchison, T.J. (2000). Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J. Cell Biol. 150, 975-988.
21. Khodjakov, A., and Rieder, C.L. (1999). The sudden recruitment of γ-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules. J. Cell Biol. 146, 585-596.
22. Kim, Y.H., Choi, J.Y., Jeong, Y., Wolgemuth, D.J., and Rhee, K. (2002). Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle. Biochem. Biophys. Res. Commun. 290, 730-736.
23. Lacey, K.R., Jackson, P.K., and Stearns, T. (1999). Cyclin-dependent kinase control of centrosome duplication. Proc. Natl. Acad. Sci. USA 96, 2817-2822.
24. Lane, H.A., and Nigg, E.A. (1996). Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plkl) in the functional maturation of mitotic centrosomes. J. Cell Biol. 135, 1701-1713.
25. Lingle, W.L., Lutz, W.H., Ingle, J.N., Maihle, N.J., and Salisbury, J.L. (1998). Centrosome hypertrophy in human breast tumors: implications for genomic stability and cell polarity. Proc. Natl. Acad. Sci. USA 95, 2950-2955.
26. Mack, G.J., Rees, J., Sandblom, O., Balczon, R., Fritzler, M.J., and Rattner, J.B. (1998). Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome. Arthritis Rheum. 41, 551-558.
27. MacRae, T.H., Lange, B.M., and Gull, K. (1990). Production and characterization of monoclonal antibodies to the mammalian sperm cytoskeleton. Mol. Reprod. Dev. 25, 384-392.
28. Mailand, N., Lukas, C., Kaiser, B.K., Jackson, P.K., Bartek, J., and Lukas, J. (2002). Deregulated human Cdc14A phosphatase disrupts centrosome separation and chromosome segregation. Nat. Cell Biol. 4, 317-322.
29. Marshall, W.F. (2001). Centrioles take center stage. Curr. Biol. 11, R487-R496.
30. Mayor, T., Hacker, U., Stierhof, Y.-D., and Nigg, E.A. (2002). The mechanism regulating dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles. J. Cell Sci. 115, 3275-3284.
31. Mayor, T., Meraldi, P., Stierhof, Y.-D., Nigg, E.A., and Fry, A.M. (1999). Protein kinases in control of the centrosome cycle. FEBS Lett. 452, 92-95.
32. Mayor, T., Tanaka, K., Stierhof, Y.-D., Fry, A.M., and Nigg, E.A. (2000). The centrosomal protein C-Nap1 displays properties supporting a role in cell cycle-regulated centrosome cohesion. J. Cell Biol. 151, 837-846.
33. -/- cells. EMBO J. 21, 483-492.
34. Meraldi, P., and Nigg, E.A. (2001). Centrosome cohesion is regulated by a balance of kinase and phosphatase activities. J. Cell Sci. 114, 3749-3757.
35. Meraldi, P., and Nigg, E.A. (2002). The centrosome cycle. FEBS Lett. 521, 9-13.
36. Nigg, E.A. (2001). Mitotic kinases as regulators of cell division and its checkpoints. Nat. Rev. Mol. Cell. Biol. 2, 21-32.
37. Nigg, E.A. (2002). Centrosome aberrations: cause or consequence of cancer progression? Nat. Rev. Cancer 2, 815-825.
38. Paintrand, M., Moudjou, M., Delacroix, H., and Bornens, M. (1992). Centrosome organization and centriole architecture: their sensitivity to divalent cations. J. Struct. Biol. 108, 107-128.
39. Piel, M., Meyer, P., Khodjakov, A., Rieder, C.L., and Bornens, M. (2000). The respective contributions of the mother and daughter centrioles to centrosome activity and behaviour in vertebrate cells. J. Cell Biol. 149, 317-329.
40. Piel, M., Nordberg, J., Euteneuer, U., and Bornens, M. (2001). Centrosome-dependent exit of cytokinesis in animal cells. Science 291, 1550-1553.
41. Pihan, G.A., Purohit, A., Wallace, J., Knecht, H., Woda, B., Quesenberry, P., and Doxsey, S.J. (1998). Centrosome defects and genetic instability in malignant tumors. Cancer Res. 58, 3974-3985.
42. Preble, A.M., Giddings, T.M., and Dutcher, S.K. (2000). Basal bodies and centrioles: their function and structure. Curr. Top. Dev. Biol. 49, 207-233.
43. Purohit, A., Tynan, S.H., Vallee, R., and Doxsey, S.J. (1999). Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization. J. Cell Biol. 147, 481-491.
44. Uto, K., and Sagata, N. (2000). Nek2B, a novel maternal form of Nek2 kinase, is essential for the assembly or maintenance of centrosomes in early Xenopus embryos. EMBO J. 19, 1816-1826.
45. Wai, D.H., Schaefer, K.L., Schramm, A., Korsching, E., Van Valen, F., Ozaki, T., Boecker, W., Schweigerer, L., Dockhorn-Dworniczak, B., and Poremba, C. (2002). Expression analysis of pediatric solid tumor cell lines using oligonucleotide microarrays. Int. J. Oncol. 20, 441-451.