Novel biotechnological applications of bacteriocins: A review

Food Control

Volumn 32, Issue 1, 2013, Pages 134-142

  Balciunas, Eduardo Marcos ^a   Castillo Martinez, Fabio Andres ^a   Todorov, Svetoslav Dimitrov ^a   Franco, Bernadette Dora Gombossy de Melo ^a   Converti, Attilio ^b   Oliveira, Ricardo Pinheiro de Souza ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITY OF GENOA   (Italy)

Author keywords

Bacteriocins; Biosafety; Biotechnological applications; Food additives; Lactic acid bacteria; Purification

Indexed keywords

BACILLUS CEREUS; BACTERIA (MICROORGANISMS); LISTERIA MONOCYTOGENES; POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 84871682216     PISSN: 09567135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodcont.2012.11.025     Document Type: Review

References (110)

1. Abee T. Pore-forming bacteriocins of Gram-positive bacteria and self-protection mechanisms of producer organism. FEMS Microbiology Letters 1995, 129:1-9.
2. Acuña L., Picariello G., Sesma F., Morero R.D., Bellomio A. A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria. Federation of European Biochemical Societies Open BIO 2012, 12-19.
3. Alisky J., Iczkowski K., Rapoport A., Troitsky N. Bacteriophages show promise as antimicrobial agents. Journal of Infection 1998, 36:5-15.
4. Ananou S., Maqueda M., Martínéz-Bueno M., Gálvéz A., Valdivia E. Control of Staphylococcus aureus in sausages by enterocin AS-48. Meat Science 2005, 71:549-556.
5. Aucher W., Lacombe C., Héquet A., Frère J., Berjeaud J.M. Influence of amino acid substitutions in the leader peptide on maturation and secretion of mesentericin Y105 by Leuconostoc mesenteroides. Journal of Bacteriology 2005, 187:2218-2223.
6. Barton M.D., Hart W.S. Public health risks: antibiotic resistance - Review. Asian-Australasian Journal of Animal Sciences 2001, 14:414-422.
7. Benkerroum N., Ghouati Y., Ghalfi H., Elmejdoub T., Roblain D., Jacques P., et al. Biocontrol of Listeria monocytogenes in a model cultured milk (lben) by in situ bacteriocin production from Lactococcus lactis ssp. lactis. International Journal of Dairy Technology 2002, 55:145-151.
8. Bhunia A.K., Johnson M.C., Ray B. Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Industrial Microbiology & Biotechnology 1987, 2:319-322.
9. Biéler S., Silva F., Belin D. The polypeptide core of microcin E492 stably associates with the mannose permease and interferes with mannose metabolism. Research in Microbiology 2010, 161:706-710.
10. Birri D.J., Brede D.A., Forberg T., Holo H., Nes I.F. Molecular and genetic characterization of a novel bacteriocin locus in Enterococcus avium isolates from infants. Applied and Environmental Microbiology 2010, 76:483-492.
11. Borrero J., Brede D.A., Skaugen M., Diep D.B., Herranz C., Nes I.F., et al. Characterization of garvicin ML, a novel circular bacteriocin produced by Lactococcus garvieae DCC43, isolated from mallard ducks (Anas platyrhynchos). Applied and Environmental Microbiology 2011, 77:369-373.
12. Brahimi M., Mathern P., Fascia P., Afchain J.M., Lucht F. Two cases of Lactobacillus rhamnosus infection and panctreatitis. Médecine et Maladies Infectieuses 2008, 38:29-31.
13. Breukink E., Wiedemann I., Van Kraaij C., Kuipers O.P., Sahl H.G., Kruijff B. Use of the cell wall precursor lipid II by a pore-forming peptide antibiotic. Science 1999, 286:2361-2364.
14. Buyong N., Kok J., Luchansky J.B. Use of a genetically enhanced, pediocin-producing starter culture, Lactococcus lactis subsp. lactis MM 217 to control Listeria monocytogenes in cheddar cheese. Applied and Environmental Microbiology 1998, 64:4842-4845.
15. Callewaert R., Holo H., Devreese B., Van Beeumen J., Nes I., De Vuyst L. Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method. Microbiology 1999, 145:2559-2568.
16. Cheigh C.I., Kook M.C., Kim S.B., Hong Y.H., Pyun Y.R. Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatography. Biotechnology Letters 2004, 26:1341-1345.
17. Chen P., Novak J., Kirk M., Barnes S., Qi F., Caufield P.W. Structure-activity study of the lantibiotic mutacin II from Streptococcus mutans T8 by a gene replacement strategy. Applied and Environmental Microbiology 1998, 64:2335-2340.
18. Cintas L.M., Casaus P., Havarstein L.S., Hernandez P.E., Nes I.F. Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Applied and Environmental Microbiology 1997, 63:4321-4330.
19. Cleveland J., Montville T.J., Nes I.F., Chikindas M.L. Bacteriocins: safe, natural antimicrobials for food preservation. International Journal of Food Microbiology 2001, 71:1-20.
20. Collins B., Cotter P.D., Hill C., Ross R.P. Applications of lactic acid bacteria-produced bacteriocins. Biotechnology of lactic acid bacteria: Novel applications 2010, 89-109. F. Mozzi, R.R. Raya, G.M. Vignolo (Eds.).
21. Cotter P.D., Hill C., Ross P. Bacteriocins: developing innate immunity for food. Nature Reviews Microbiology 2005, 3:777-788.
22. Cunningham-Rundles S., Ahrne S., Bengmark S., Johann-Liang R., Marshall F., Metakis L., et al. Probiotics and immune response. American Journal of Gastroenterology 2000, 95:22-25.
23. Delves-Broughton J. Nisin and its application as a food preservative. Journal of the Society of Dairy Technology 1990, 43:73-76.
24. Díez L., Rojo-Bezares B., Zarazaga M., Rodríguez J.M., Torres C., Ruiz-Larrea F. Antimicrobial activity of pediocin PA-1 against Oenococcus oeni and other wine bacteria. Food Microbiology 2012, 31:167-172.
25. Drider D., Fimland G., Hechard Y., Mcmullen L.M., Prevost H. The continuing story of class IIa bacteriocins. Microbiology and Molecular Biology Reviews 2006, 70:564-582.
26. Drosinos E.H., Mataragas M., Nasis P., Galiotou M., Metaxopoulos J. Growth and bacteriocin production kinetics of Leuconostoc mesenteroides E131. Journal of Applied Microbiology 2005, 99:1314-1323.
27. Duquesne S., Destoumieux-Garzón D., Peduzzi J., Rebuffat S. Microcins, gene-encoded antibacterial peptides from enterobacteria. Natural Product Reports 2007, 24:708-734.
28. Elotmani F., Revol-Junelles A.M., Assobhei O., Milliére J. Characterization of anti-Listeria monocytogenes bacteriocins from Enterococcus faecalis, Enterococcus faecium and Lactococcus lactis strains isolated from Raib, a Moroccan traditional fermented milk. Current Microbiology 2002, 44:10-17.
29. Ennahar S., Aoude-Werner D., Sorokine O., Dorsselaer A.V., Bringel F., Hubert J.C., et al. Production of pediocin AcH by Lactobacillus plantarum WHE92 isolated from cheese. Applied and Environmental Microbiology 1996, 62:4381-4387.
30. Ercolini D., Storia A., Villani F., Mauriello G. Effect of a bacteriocin-activated polythene film on Listeria monocytogenes as evaluated by viable staining and epifluorescence microscopy. Journal of Applied Microbiology 2006, 100:765-772.
31. Farías M.E., Farías R.N., de Ruiz Holgado A.P., Sesma F. Purification and N-terminal amino acid sequence of enterocin CRL 35, a " pediocin-like" bacteriocin produced by Enterococcus faecium CRL 35. Letters in Applied Microbiology 1996, 22:417-419.
32. Fernández L., Delgado S., Herrero H., Maldonado A., Rodríguez J.M. The bacteriocin nisin, as effective agent for the treatment of Staphylococcal mastitis during lactation. Journal of Human Lactation 2008, 24:311-316.
33. Fimland G., Johnsen L., Dalhus B., Nissen-Meyer J. Pediocin-like antimicrobial peptides (class IIa bacteriocins) and their immunity proteins: biosynthesis, structure and mode of action. Journal of Peptide Science 2005, 11:688-696.
34. Foulquié-Moreno M.R., Callewaert R., De Vuyst L. Isolation of bacteriocins through expanded bed adsorption using a hydrophobic interaction medium. Bioseparation 2001, 10:45-50.
35. Franz C.M.A.P., Cho G.S., Holzapfel W.H., Gálvez A. Safety of lactic acid bacteria. Biotechnology of lactic acid bacteria: Novel applications 2010, 341-359. F. Mozzi, R.R. Raya, G.M. Vignolo (Eds.).
36. Gallemore G.H., Mohon R.T., Ferguson D.A. Lactobacillus fermentum endocarditis involving a native mitral valve. Journal of the Tennessee Medical Association 1995, 88:306-308.
37. Garneau S., Martin N.I., Vederas J.C. Two-peptide bacteriocins produced by lactic acid bacteria. Biochimie 2002, 84:577-592.
38. Georgalaki M.D., Van Den Berghe E., Kritikos D., Devreese B., Van Beeumen J., Kalantzopoulos G., et al. Macedocin, a food-grade lantibiotic produced by Streptococcus macedonicus ACA-DC 198. Applied and Environmental Microbiology 2002, 68:5891-5903.
39. Gérard F., Pradel N., Wu L.F. Bactericidal activity of colicin V is mediated by an inner membrane protein, SdaC, of Escherichia coli. Journal of Bacteriology 2005, 187:1945-1950.
40. Gillor O., Nigro L.M., Riley M.A. Genetically engineered bacteriocins and their potential as the next generation of antimicrobials. Current pharmaceutical design 2005, 11:1067-1075.
41. Gratia A. Sur un remarquable exemple d'antagonisme entre deux souches de coilbacille. Comptes Rendus des Séances et Mémoires de la Société de Biologie 1925, 93:1040-1041.
42. Gravesen A., Axelsen A.M.J., Silva J.M., Hansen T.B., Knochel S. Frequency of bacteriocin resistance development and associated fitness costs in Listeria monocytogenes. Applied and Environmental Microbiology 2002, 68:756-764.
43. Guinane C.M., Cotter P.D., Hill C., Ross R.P. Microbial solutions to microbial problems; lactococcal bacteriocins for the control of undesirable biota in food. Journal of Applied Microbiology 2005, 98:1316-1325.
44. Hernandez P.E., Rodriguez J.M., Cintas L.M., Moreira W.L., Sobrino O.J., Fernandez M.F., et al. Utilización de bactérias lácticas en el control de microorganismos patógenos de los alimentos. Microbiologia SEM 1993, 9:37-48.
45. Hoffmann A., Schneider T., Pag U., Sahl H.G. Localization and functional analysis of PepI, the immunity peptide of Pep 5-producing Staphylococcus epidermidis strain 5. Applied and Environmental Microbiology 2004, 70:3263-3271.
46. Holzapfel W.H. Appropriate starter culture technologies for small-scale fermentation in developing countries. International Journal of Food Microbiology 2002, 75:197-212.
47. Holzapfel W.H., Geisen R., Schillinger U. Biological preservation of foods with reference to protective cultures, bacteriocins and food-grade enzymes. International Journal of Food Microbiology 1995, 24:343-362.
48. Hu C.B., Malaphan W., Zendo T., Nakayama J., Sonomoto K. Enterocin X, a novel twopeptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides. Applied and Environmental Microbiology 2010, 76:4542-4545.
49. Hugas M., Garriga M., Pascual M., Aymerich M.T., Monfort J.M. Enhancement of sakacin K activity against Listeria monocytogenes in fermented sausages with pepper or manganese as ingredients. Food Microbiology 2002, 19:519-528.
50. Ishibashi N., Yamazaki S. Probiotics and safety. American Journal of Clinical Nutrition 2001, 73:465-470.
51. Jarvis B., Jeffcoat J., Cheeseman G.C. Molecular weight distribution of nisin. Biochemical Biophysiologie Acta 1968, 168:153-155.
52. Joerger R.D. Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages. Poultry Science 2003, 82:640-647.
53. Jozala A.F., Andrade M.S., Arauz L.J., Pessoa A., Vessoni-Penna T.C. Nisin production utilizing skimmed milk aiming to reduce process cost. Applied Biochemical Biotechnology 2007, 136:515-528.
54. Kaiser A.L., Montville T.J. Purification of the bacteriocin bavaricin MN and characterization of its mode of action against Listeria monocytogenes Scott A cells and lipid vesicles. Applied and Environmental Microbiology 1996, 62:4529-4535.
55. Kawai Y., Ishii Y., Arakawa K., Uemura K., Saitoh B., Nishimura J., et al. Structural and functional differences in two cyclic bacteriocins with the same sequences produced by lactobacilli. Applied and Environmental Microbiology 2004, 70:2906-2911.
56. Kazazic M., Nissen-Meyer J., Fimland G. Mutational analysis of the role of changed residues in target-cell binding, potency and specificity of the pediocin-like bacteriocin sakacin P. Microbiology 2002, 148:2019-2027.
57. Kelly W.J., Asmundson R.V., Huang C.M. Characterization of plantaricin KW30, a bacteriocin produced by Lactobacillus plantarum. Journal of Applied Bacteriology 1996, 81:657-662.
58. Kjems E. Studies on streptococcal bacteriophages: I. Techniques for isolating phage producing strains. Pathology and Microbiology Scandinavia 1955, 36:433-440.
59. Klaenhammer T.R. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiological Review 1993, 12:39-85.
60. Kleerebezem M., Quadri L.E. Peptide pheromone-dependent regulation of antimicrobial peptide production in Gram-positive bacteria: a case of multicellular behavior. Peptides 2001, 22:1579-1596.
61. Kluskens L.D., Kuipers A., Rink R., De Boef E., Fekken S., Driessen A.J., et al. Posttranslational modification of therapeutic peptides by NisB, the dehydratase of the lantibiotic nisin. Biochemistry 2005, 44:12827-12834.
62. Knowles D.J.C. New strategies for antibacterial drug design. Trends in Microbiology 1997, 5:379-383.
63. Lai A.C., Tran S., Simmonds R.S. Functional characterization of domains found within a lytic enzyme produced by Streptococcus equi subsp. zooepidemicus. FEMS Microbiology Letters 2002, 215:133-138.
64. Leroy F., De Vuyst L. The presence of salt and a curing agent reduces bacteriocin production by Lactobacillus sakei CTC 494, a potential starter culture for sausage fermentation. Applied and Environmental Microbiology 1999, 65:5350-5356.
65. Leroy F., Verluyten J., De Vuyst L. Functional meat starter cultures for improved sausage fermentation. International Journal of Food Microbiology 2006, 106:270-285.
66. Levin B.R., Antia R., Berliner E., Bloland P., Bonhoeffer S., Cohen M. Resistance to antimicrobial chemotherapy: a prescription for research and action. American Journal of the Medical Sciences 1998, 315:87-94.
67. Li C., Bai J., Cai Z., Ouyang F. Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. Journal of Biotechnology 2002, 93:27-34.
68. Lien S., Lowman H.B. Therapeutic peptides. Trends in Biotechnology 2003, 21:556-562.
69. Loessner M., Guenther S., Steffan S., Scherer S. A pediocin-producing Lactobacillus plantarum strain inhibits Listeria monocytogenes in a multispecies cheese surface microbial ripening consortium. Applied and Environmental Microbiology 2003, 69:1854-1857.
70. López R.L., García M.T., Abriouel H., Omar N.B., Grande M.J., Martínez-Cañamero M., et al. Semi-preparative scale purification of enterococcal bacteriocin enterocin EJ97, and evaluation of substrates for its production. Journal of Industrial Microbiology & Biotechnology 2007, 34:779-785.
71. Macfarlane G.T., Cummings J.H. Probiotics, infection and immunity. Current Opinion in Infectious Diseases 2002, 15:501-506.
72. Macwana S., Muriana P.M. Spontaneous bacteriocin resistance in Listeria monocytogenes as a susceptibility screen for identifying different mechanisms of resistance and modes of action by bacteriocins of lactic acid bacteria. Journal of Microbiological Methods 2012, 88:7-13.
73. Mantovani H.C., Russel J.B. Nisin resistance of Sreptococcus bovis. Applied and Environmental Microbiology 2001, 67:808-813.
74. Mayo B., Aleksandrzak-Piekarczyk T., Fernández M., Kowalczyk M., álvarez-Martín P., Bardowski J. Updates in the metabolism of lactic acid bacteria. Biotechnology of lactic acid bacteria: Novel applications 2010, 3-33. Wiley-Blackwell, Iowa, USA. F. Mozzi, R.R. Raya, G.M. Vignolo (Eds.).
75. Mcmanus P.S., Stockwell V.O., Sundin G.W., Jones A.L. Antibiotic use in plant agriculture. Annual Review of Phytopathology 2002, 40:443-465.
76. Mills S., Stanton C., Hill C., Ross R.P. New developments and applications of bacteriocins and peptides in foods. Annual Review of Food Science and Technology 2011, 2:299-329.
77. Mulders J.W., Boerrigter I.J., Rollema H.S., Siezen R.J., Vos W.M. Identification and characterization of the lantibiotic nisin Z, a natural nisin variant. European Journal of Biochemistry 1991, 201:581-584.
78. Naghmouchi K., Le Lay C., Baah J., Drider D. Antibiotic and antimicrobial peptide combinations: synergistic inhibition of Pseudomonas fluorescens and antibiotic-resistant variants. Research in Microbiology 2012, 10.1016/j.resmic.2011.11.002.
79. Nascimento M.S., Moreno I., Kuaye A.Y. Bacteriocinas em alimentos: uma revisão. Brazilian Journal of Food Technology 2008, 11:120-127.
80. Nattress F.M., Yost C.K., Baker L.P. Evaluation of the ability of lysozyme and nisin to control meat spoilage bacteria. International Journal of Food Microbiology 2001, 70:111-119.
81. Nes I.F., Eijsink V.G.H. Regulation of group II peptide bacteriocin synthesis by quorum-sensing mechanisms. Cell-cell signalling in bacteria 1999, 175-192. American Society for Microbiology. G.M. Dunny, S.C. Winans (Eds.).
82. Nilsen T., Nes I.F., Holo H. Na exported inducer peptide regulates bacteriocin production in Enterococcus faecium CTC 492. Journal of Bacteriology 1998, 180:1848-1854.
83. Oguntoyinbo F.A., Narbad A. Molecular characterization of lactic acid bacteria and in situ amylase expression during traditional fermentation of cereal foods. Food Microbiology 2012, 31:254-262.
84. O'Mahony T., Rekhif N., Cavadini C., Fitzgerald G.F. The application of a fermented food ingredient containing 'variacin', a novel antimicrobial produced by Kocuria varians, to control the growth of Bacillus cereus in chilled dairy products. Journal of Applied Microbiology 2001, 90:106-114.
85. O'Sullivan L., Ryan M.P., Ross R.P., Hill C. Generation of food-grade lactoccal starters which produce the lantibiotics lacticin 3147 and lacticin 481. Journal of Applied and Environmental Microbiology 2003, 69:3681-3685.
86. Parente E., Ricciardi A. Production, recovery and purification of bacteriocins from lactic acid bacteria. Journal of Applied Microbiology and Biotechnology 1999, 52:628-638.
87. Phillips J.D., Griffths M.W., Muir D.D. Effect of nisin on the shelf-life of pasteurized double cream. The Journal of the Society of the Dairy Technology 1983, 36:17-21.
88. Pons A.M., Lanneluc I., Cottenceau G., Sable S. New developments in non-post translationally modified microcins. Biochimie 2002, 84:531-537.
89. Prioult G., Turcotte C., Labarre L., Lacroix C., Fliss I. Rapid purification of nisin Z using specific monoclonal antibody-coated magnetic beads. International Dairy Journal 2000, 10:627-633.
90. Rilla N., Martinez B., Rodriguez A. Inhibition of a methicillin-resistant Staphylococcus aureus strain in Afuega'I Pitu cheese by the nisin Z producing strain Lactococcus lactis lactis IPLA 729. Journal of Food Protection 2004, 67:928-933.
91. Rink R., Kluskens L.D., Kuipers A., Driessen A.J., Kuipers O.P., Moll G.N. NisC, the cyclase of the lantibiotic nisin, can catalyze cyclization of designed nonlantibiotic peptides. Biochemistry 2007, 46:13179-13189.
92. Rollema H.S., Kuipers O.P., Both P., De Vos W.M., Siezen R.J. Improvement of solubility and stability of the antimicrobial peptide nisin by protein engineering. Journal of Applied and Environmental Microbiology 1995, 61:2873-2878.
93. Ross R.P., Morgan S., Hill C. Preservation and fermentation: past, present and future. International Journal of Food Microbiology 2002, 79:3-16.
94. Ryan M.P., Mcauliffe O., Ross R.P., Hill C. Heterologous expression of lacticin 3147 in Enterococcus faecalis: comparison of biological activity with cytolysin. Letters in Applied Microbiology 2001, 32:71-77.
95. Sarantinopoulos P., Leroy F., Leontopoulou E., Georgalaki M.D., Kalantzopoulos G., Tsakalidou E., et al. Bacteriocin production by Enterococcus faecium FAIR-E 198 in view of its application as adjunct starter in Greek Feta cheese making. International Journal of Food Microbiology 2002, 72:125-136.
96. Schillinger U., Geisen R., Holzapfel W.H. Potential of antagonistic microorganisms and bacteriocins for the biological preservation of foods. Trends Food Science and Technology 1996, 7(5):158-164.
97. Sherley M., Gordon D.M., Collignon P.J. Variations in antibiotic resistance profile in Enterobacteriaceae isolated from wild Australian mammals. Environmental Microbiology 2000, 2:620-631.
98. da Silva-Malheiros P., Daroit D.J., da Silveira N.P., Brandelli A. Effect of nanovesicle-encapsulated nisin on growth of Listeria monocytogenes in milk. Food Microbiology 2010, 27:175-178.
99. Simha B.V., Sood S.K., Kumariya R., Garsa A.K. Simple and rapid purification of pediocin PA-1 from Pediococcus pentosaceous NCDC 273 suitable for industrial application. Microbiological Research 2012, 10.1016/j.micres.2012.01.001.
100. Songisepp E., Hütt P., Rätsep M., Shkut E., Kõljalg S., Truusalu K., et al. Safety of a probiotic cheese containing Lactobacillus plantarum Tensia according to a variety of health indices in different age groups. Journal of Dairy Science 2012, 95:5495-5509.
101. Stiles M.E. Biopreservation by lactic acid bacteria. Antonie Van Leeuwenhoek 1996, 70:331-345.
102. Suda S., Westerbeek A., O'Connor P.M., Ross R.P., Hill C., Cotter P.D. Effect of bioengineering lacticin 3147 lanthionine bridges on specific activity and resistance to heat and proteases. Chemistry & Biology 2010, 17:1151-1160.
103. Taneja N., Rani P., Emmanuel R., Khudaier B.Y., Sharma S.K., Tewari R., et al. Nosocomial urinary tract infection due to Leuconostoc mesenteroides at a tertiary care centre in north india. Indian Journal of Medical Research 2005, 122:178-179.
104. Twomey D., Ross R.P., Ryan M., Meaney B., Hill C. Lantibiotics produced by lactic acid bacteria: structure, function and applications. Antonie Van Leeuwenhoek 2002, 82:165-185.
105. Vadyvaloo V., Hastings J.W., Van Der Merwe M.J., Rautenbach M. Membranes of class IIa bacteriocin-resistant L. monocytogenes cells contain increased levels of desaturated and snort-acyl-chain phosphatidylglycerols. Applied and Environmental Microbiology 2002, 68:5223-5230.
106. Van Houten M.A., Luinge K., Laseur M., Kimpen J.L. Antibiotic utilisation for hospitalised paediatric patients. International Journal of Antimicrobial Agents 1998, 10:161-164.
107. Van Schaik W., Gahan C.G., Hill C. Acid-adapted Listeria monocytogenes displays enhanced tolerance against the lantiobiotics nisin and lacticin 3147. Journal of Food Protection 1999, 62:536-540.
108. Vaucher R.A., Gewehr C.C.V., Correa A.P.F., Sant'Anna V., Ferreira J., Brandelli A. Evaluation of the immunogenicity and in vivo toxicity of the antimicrobial peptide P34. International Journal of Pharmaceutics 2011, 421:94-98.
109. Wester C.W., Durairaj L., Evans A.T., Schwartz D.N., Husain S., Martinez E. Antibiotic resistance - a survey of physician perceptions. Archives of Internal Medicine 2002, 162:2210-2216.
110. Yuan J., Zhang Z.Z., Chen X.Z., Yang W., Huan L.D. Site-directed mutagenesis of the hinge region of nisin Z and properties of nisin Z mutants. Applied Microbiology and Biotechnology 2004, 64:806-815.