Biochemical and genetic characterization of enterocin P, a novel sec- dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum

Applied and Environmental Microbiology

Volumn 63, Issue 11, 1997, Pages 4321-4330

  Cintas, Luis M ^a,b   Casaus, Pilar ^a,b   Håvarstein, Leiv Sigve ^b   Hernández, Pablo E ^a   Nes, Ingolf F ^b  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOCIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERICIDAL ACTIVITY; ENTEROCOCCUS FAECALIS; NONHUMAN; PROTEIN ANALYSIS; PROTEIN ISOLATION;

EID: 0030698620     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.11.4321-4330.1997     Document Type: Article

References (67)

1. Allison, G. E., C. Ahn, M. E. Stiles, and T. K. Klaenhammer. 1995. Utilization of the leucocin A export system in Leuconostoc gelidum for production of a Lactobacillus bacteriocin. FEMS Microbiol. Lett. 131:87-93.
2. Allison, G. E., C. Ahn, M. E. Stiles, and T. R. Klaenhammer. 1995. Heterologous expression of the lactacin F peptides by Carnobacterium piscicola LV17. Appl. Environ. Microbiol. 61:1371-1377.
3. Anderson, D. G., and L. L. McKay. 1983. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl. Environ. Microbiol. 46:549-552.
4. Axelsson, L., and A. Holck. 1995. The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706. J. Bacteriol. 177:2125-2137.
5. Aymerich, T., H. Holo, L. S. Håvarstein, M. Hugas, M. Garriga, and I. F. Nes. 1996. Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin-family of bacteriocins. Appl. Environ. Microbiol. 62:1676-1682.
6. Ben Embarek, P. K., V. From Jeppesen, and H. H. Hus. 1994. Antibacterial potential of Enterococcus faecium strains isolated from sous-vide cooked fish fillets. Food Microbiol. 11:525-536.
7. Casaus, M. P., L. M. Cintas, A. Suárez, J. M. Rodríguez, H. Holo, P. E. Hernández, and I. F. Nes. 1995. Enterococcus faecium T136: a two anti-Listeria active bacteriocins producer, isolated from Spanish dry fermented sausage, abstr. p-156. In European Commission Biotechnology and Fair Programmes Conference on Lactic Acid Bacteria.
8. Casaus, P., T. Nilsen, L. M. Cintas, I. F. Nes, P. E. Hernández, and H. Holo. 1997. Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A. Microbiology 143:2287-2294.
9. Chikindas, M. L., M. J. García, Garcerá, A. J. M. Driessen, A. M. Ledeboer, J. Nissen-Meyer, I. F. Nes, T. Abee, W. N. Konings, and G. Venema. 1993. Pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC1.0, forms hydrophilic pores in the cytoplasmic membrane of target cells. Appl. Environ. Microbiol. 59:3577-3584.
10. Cintas, L. M., J. M. Rodríguez, M. F. Fernández, K. Sletten, I. F. Nes, P. E. Hernández, and H. Holo. 1995. Isolation and characterization of pediocin L50, a new bacteriocin from Pediococcus acidilactici with a broad inhibitory spectrum. Appl. Environ. Microbiol. 61:2643-2648.
11. Cornwell, G. G., K. Sletten, B. Johansson, and P. Westemark. 1988. Evidence that the amyloid fibril protein in senile systemic amyloidosis is derived from normal prealbumin. Biochem. Biophys. Res. Commun. 154:648-653.
12. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
13. Devriese, L. A., B. Pot, and M. D. Collins. 1993. Phenotyphic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J. Appl. Bacteriol. 75:399-408.
14. de Vuyst, L., and E. J. Vandamme. 1994. Bacteriocins of lactic acid bacteria. Blackie Academie & Professional, London, England.
15. Diep, D. B., L. S. Håvarstein, and I. F. Nes. 1996. Characterization of the locus responsible for the bacteriocin production in Lactobacillus plantarum C11. J. Bacteriol. 178:4472-4483.
16. Farías, M. E., R. N. Farías, A. P. de Ruíz Holgado, and F. Sesma. 1996. Purification and N-terminal amino acid sequence of enterocin CRL35, a "pediocin-like" bacteriocin produced by Enterococcus faecium CRL35. Lett. Appl. Microbiol. 22:417-419.
17. Fath, M. J., L. H. Zhang, J. Rush, and R. Kolter. 1994. Purification and characterization of colicin V from Escherichia coli culture supernatants. Biochemistry 33:6911-6917.
18. Fimland, G., O. R. Blingsmo, K. Sletten, G. Jung, I. F. Nes, and J. Nissen-Meyer. 1996. New biologically active hybrid bacteriocins constructed by combining regions from various pediocin-like bacteriocins: the C-terminal region is important for determining specificity. Appl. Environ. Microbiol. 62:3313-3318.
19. Franke, C. M., K. J. Leenhouts, A. J. Haandrikman, J. Kok, G. Venema, and K. Venema. 1996. Topology of LcnD, a protein implicated in the transport of bacteriocins from Lactococcus lactis. J. Bacteriol. 178:1765-1769.
20. Franz, C. M. A. P., R. W. Worobo, L. E. N. Quadri, U. Schillinger, W. H. Holzapfel, and M. E. Stiles. 1996. Purification of enterocin 900, a bacteriocin produced by Enterococcus faecium BFE900, abstr. C-27. In Abstracts of the 5th Symposium on Lactic Acid Bacteria.
21. Gierasch, L. M. 1989. Signal sequences. Biochemistry 28:923-930.
22. Giraffa, G. 1995. Enterococcal bacteriocins: their potential as anti-Listeria factors in dairy technology. Food Microbiol. 12:291-299.
23. Hastings, J. W., M. Sailer, K. Johnson, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1991. Characterization of leucocin A-UAL187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J. Bacteriol. 173:7491-7500.
24. Håvarstein, L. S., H. Holo, and I. F. Nes. 1994. The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by gram-positive bacteria. Microbiology 140: 2383-2389.
25. Håvarstein, L. S., D. B. Diep, and I. F. Nes. 1995. A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export. Mol. Microbiol. 16:229-240.
26. Héchard, Y., B. Derijard, F. Letellier, and Y. Cenatiempo. 1992. Characterization and purification of mesentericin Y105, an anti-Listeria bacteriocin from Leuconostoc mesenteroides. J. Gen. Microbiol. 138:2725-2731.
27. Henderson, J. T., A. L. Chopko, and P. D. van Wassenaar. 1992. Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0. Arch. Biochem. Biophys. 295:5-12.
28. Holck, A., L. Axelsson, S.-E. Birkeland, T. Aukrust, and H. Blom. 1992. Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706. J. Gen. Microbiol. 138:2715-2720.
29. Holck, A. L., L. Axelsson, and U. Schillinger. 1994. Purification and cloning of piscicolin 61, a bacteriocin from Carnobacterium piscicola LV61. Curr. Microbiol. 29:63-68.
30. Holo, H., Ø. Nilssen, and I. F. Nes. 1991. Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene. J. Bacteriol. 173:3879-3887.
31. Hoover, G. D., and L. R. Steenson. 1993. Bacteriocins of lactic acid bacteria. Academic Press, Inc., San Diego, Calif.
32. Izard, J. W., and D. A. Kendall. 1994. Signal peptides: exquisitely designed transport promoters. Mol. Microbiol. 13:765-773.
33. Jack, R. W., J. R. Tagg, and B. Ray. 1995. Bacteriocins of gram-positive bacteria. Microbiol. Rev. 59:171-200.
34. Kersters, K., and J. de Ley. 1975. Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. J. Gen. Microbiol. 87:333-342.
35. Klaenhammer, T. R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12:39-86.
36. Kok, J., H. Holo, M. van Belkum, A. Haandrikman, and I. F. Nes. 1993. Non-nisin bacteriocins in lactococci: biochemistry, genetics and mode of action, p. 121-151. In D. Hoover and L. Steensen (ed.), Bacteriocins in lactic acid bacteria. Academic Press, Inc., New York, N.Y.
37. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
38. Larsen, A. G., F. K. Vogensen, and J. Josephsen. 1993. Antimicrobial activity of lactic acid bacteria isolated from sour doughs: purification and characterization of bavaricin A, a bacteriocin produced by Lactobacillus bavaricus MI401. J. Appl. Bacteriol. 75:113-122.
39. Lauková, A., M. Mareková, and P. Javorsky. 1993. Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231. Lett. Appl. Microbiol. 16:257-260.
40. Leer, R. J., J. M. B. M. van der Vossen, M. van Giezen, J. M. van Noort, and P. H. Pouwels. 1995. Genetic analysis of acidocin B, a novel bacteriocin produced by Lactobacillus acidophilus. Microbiology 141:1629-1635.
41. McCormick, J. K., R. W. Worobo, and M. E. Stiles. 1996. Expression of the antimicrobial peptide carnobacteriocin B2 by a signal peptide-dependent general secretory pathway. Appl. Environ. Microbiol. 62:4095-4099.
42. McKay, A. M. 1990. Antimicrobial activity of Enterococcus faecium against Listeria spp. Lett. Appl. Microbiol. 11:15-17.
43. Nes, I. F., D. Bao Diep, L. S. Håvarstein, M. B. Brurberg, V. Eijsink, and H. Holo. 1996. Biosynthesis of bacteriocins in lactic acid bacteria. Antonie Leeuwenhoek 70:113-128.
44. Nieto-Lozano, J. C., J. Nissen-Meyer, K. Sletten, C. Peláez, and I. F. Nes. 1992. Purification and amino acid sequence of a bacteriocin produced by Pediococcus acidilactici. J. Gen. Microbiol. 138:1-6.
45. Olasupo, N. A., U. Schillinger, C. M. A. P. Franz, and W. H. Holzapfel. 1994. Bacteriocin production by Enterococcus faecium NA01 from "wara" - a fermented skimmed cow milk product from West Africa. Lett. Appl. Microbiol. 19:438-441.
46. Pot, B., P. Vandamme, and K. Kersters. 1994. Analysis of electrophoretic whole-organism protein fingerprints, p. 493-521. In M. Goodfellow and A. G. O'Donnell (ed.), Chemical methods in prokaryotic systematics. J. Wiley & Sons, Inc., Chichester, England.
47. Ptashne, M. 1992. The genetic switch. Cell Press & Blackwell Scientific Publications, Cambridge, Mass.
48. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
49. Quadri, L. E. N., M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1994. Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV17B. J. Biol. Chem. 269:12204-12211.
50. Reuter, G. 1970. Laktobazillen und engverwandte Mikroorganismen in Fleisch und Fleischerzeugnissen. 2. Mitteilung: die Charakterisierung der isolierten Laktobazillen-Stämme. Fleischwirtschaft 47:397-402.
51. Rodríguez, J. M., L. M. Cintas, P. Casaus, A. Suárez, and P. E. Hernández. 1995. PCR detection of the lactocin S structural gene in bacteriocin-producing lactobacilli from meat. Appl. Environ. Microbiol. 61:2802-2805.
52. Rodríguez, J. M., L. M. Cintas, M. P. Casaus, N. Horn, H. M. Dodd, P. E. Hernández, and M. J. Gasson. 1995. Isolation of nisin-producing Lactococcus strains from dry fermented sausages. J. Appl. Bactcriol. 78:109-115.
53. Schillinger, U., and F.-K. Lücke. 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55:1901-1906.
54. Schleifer, K. H., and R. Kilpper-Bälz. 1984. Transfer of Streptococcus faecalis and S. feacium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int. J. Syst. Bacteriol. 34:31-34.
55. Siragusa, G. R. 1992. Production of bacteriocin inhibitory to Listeria species by Enterococcus hirae. Appl. Environ. Microbiol. 58:3508-3513.
56. Sobrino, O. J., J. M. Rodriguez, W. L. Moreira, M. F. Fernández, B. Sanz, and P. E. Hernández. 1991. Antibacterial activity of Lactobacillus sake isolated from dry fermented sausages. Int. J. Food Microbiol. 13:1-10.
57. Tichaczek, P. S., J. Nissen-Meyer, I. F. Nes, R. F. Vogel, and W. P. Hammes. 1992. Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from Lactobacillus sake LTH673. Syst. Appl. Microbiol. 15:460-468.
58. Tomita, H., S. Fujimoto, K. Tanimoto, and Y. Ike. 1996. Cloning and genetic organization of the bacteriocin 31 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pY117. J. Bacteriol. 178: 3585-3593.
59. Torri Tarelli, G., D. Carminati, and G. Giraffa. 1994. Production of bacteriocins active against Listeria monocytogenes and Listeria innocua from dairy enterococci. Food Microbiol. 11:242-252.
60. van Belkum, M. J., and M. E. Stiles. 1995. Molecular characterization of genes involved in the production of the bacteriocin leucocin A from Leuconostoc gelidum. Appl. Environ. Microbiol. 61:3573-3579.
61. van Belkum, M. J., R. W. Worobo, and M. E. Stiles. 1997. Double-glycine-type leader peptides direct secretion of bacteriocins by ABC transporters: colicin V secretion in Lactococcus lactis. Mol. Microbiol. 23:1293-1301.
62. Villani, F., G. Salazano, E. Sorrentino, O. Pepe, P. Marino, and S. Coppola. 1993. Enterocin 226NWC, a bacteriocin produced by Enterococcus faecalis 226, active against Listeria monocytogenes. J. Appl. Bacteriol. 74:380-387.
63. Vlaemynck, G., L. Herman, and K. Coudijzer. 1993. Isolation and characterization of two bacteriocins produced by Enterococcus faecium strains inhibitory to Listeria monocytogenes. Int. J. Food Microbiol. 24:211-225.
64. von Heijne, G. 1983. Patterns of amino acids near signal sequence cleavage sites. Eur. J. Biochem. 133:17-21.
65. von Heijne, G. 1986. Net N-C charge imbalance may be important for signal sequence function in bacteria. J. Mol. Biol. 192:287-290.
66. Worobo, R. W., T. Henkel, M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1994. Characteristics and genetic determinant of a hydrophobic peptide bacteriocin, carnobacteriocin A, produced by Carnobacterium piscicola LV17A. Microbiology 140:517-526.
67. Worobo, R. W., M. J. van Belkum, M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1995. A signal peptide secretion-dependent bacteriocin from Carnobacterium divergens. J. Bacteriol. 177:3143-3149.