Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatography

Biotechnology Letters

Volumn 26, Issue 17, 2004, Pages 1341-1345

  Cheigh, Chan Ick ^a   Kook, Moo Chang ^a   Kim, Seong Bo ^a   Hong, Young Ho ^a   Pyun, Yu Ryang ^a  

^a Yonsei University   (South Korea)

Author keywords

Chromatography; Expanded bed; Lactococcus lactis; Nisin Z; Purification

Indexed keywords

ADSORBENTS; BACTERIOLOGY; CHROMATOGRAPHY; FRACTIONATION; ION EXCHANGE; PH EFFECTS; PURIFICATION;

EXPANDED BEDS; ION EXCHANGE CHROMATOGRAPHY; LACTOCOCCUS LACTIS; NISIN Z;

BACTERIA;

LACTOCOCCUS LACTIS; LACTOCOCCUS LACTIS SUBSP. LACTIS;

BACTERIAL PROTEIN; DRUG DERIVATIVE; NISIN; NISIN Z;

ARTICLE; CHEMISTRY; GROWTH, DEVELOPMENT AND AGING; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; LACTOCOCCUS LACTIS;

BACTERIAL PROTEINS; CHROMATOGRAPHY, ION EXCHANGE; LACTOCOCCUS LACTIS; NISIN;

EID: 17644420570     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000045630.29494.45     Document Type: Article

References (13)

1. Bhunia AK, Johnson MC, Ray B (1987) Direct detection of an anti-microbial peptide of Pediococcus acidilactici in sodium dodesyl sulfate polyacrylamide gel electrophoresis. J. Ind. Microbiol. 2: 319-322.
2. Carolissen-Mackay V, Arendse G, Hastings JW (1997) Purification of bacteriocins of lactic acid bacteria: problems and pointers. Int. J. Food Microbiol. 34: 1-16.
3. Chang YK, Chase HA (1996a) Ion exchange purification of G6PDH from unclarified yeast cell homogenates using expanded bed adsorption. Biotechnol. Bioeng. 49: 204-216.
4. Chang YK, Chase HA (1996b) Development of operating conditions for protein purification using expanded bed techniques: the effect of the degree of bed expansion on adsorption performance. Biotechnol. Bioeng. 49: 512-526.
5. Cheigh CI, Choi HJ, Park H, Kim SB, Kook MC, Kim TS, Hwang JK, Pyun YR (2002) Influence of growth conditions on the production of a nisin-like bacteriocin by lactococcus lactis subsp. lactis A164 isolated from kimchi. J. Biotechnol. 95: 225-235.
6. Choi HJ, Cheigh CI, Kim SB, Pyun YR (2000) Production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from Kimchi. J. Appl. Microbiol. 88: 1-11.
7. De Vuyst L, Vandamme EJ (1994) Bacteriocins of Lactic Acid Bacteria; Microbiology, Genetics and Applications. London: Blackie Academic & Professional.
8. Hu HB, Yao SJ, Mei LH, Zhu ZQ, Hur BK (2000) Partial purification of nattokinase from Bacillus subtilis by expanded bed adsorption. Biotechnol. Lett. 22: 1383-1387.
9. Jack RW, Tagg JR, Ray B (1995) Bacteriocins of Gram-positive bacteria. Microbiol. Rev. 59: 171-200.
10. Mullick A, Flickinger MC (1999) Expanded bed adsorption of human serum albumin from very dense Saccharomyces cerevesiae suspensions on fluoride-modified zirconia. Biotechnol. Bioeng. 65: 282-290.
11. Parente E, Ricciardi A (1999) Production, recovery and purification of bacteriocins from lactic acid bacteria. Appl. Microbiol. Biotechnol. 52: 628-638.
12. Ray B, Daeschel MA (1992) Food Biopreservatives of Microbial Origin. Boca Raton, FL: CRC Press.
13. Yang R, Johnson MC, Ray B (1992) Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 58: 3355-3359.