Purification of the bacteriocin bavaricin MN and characterization of its mode of action against Listeria monocytogenes scott A cells and lipid vesicles

Applied and Environmental Microbiology

Volumn 62, Issue 12, 1996, Pages 4529-4535

  Kaiser, Alan L ^a,c   Montville, Thomas J ^a,b  

^a RUTGERS UNIVERSITY   (United States)

^b RUTGERS UNIVERSITY   (United States)

^c NATIONAL JEWISH MEDICAL AND RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOCIN; BAVARICIN MN; CARBOXYFLUORESCEIN; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION RESPONSE; LACTOBACILLUS; LIPID VESICLE; LISTERIA MONOCYTOGENES; MEMBRANE POTENTIAL; MOLECULAR WEIGHT; PROTEIN PURIFICATION; SEQUENCE ANALYSIS;

LACTOBACILLUS; LACTOBACILLUS SAKEI; LISTERIA MONOCYTOGENES;

EID: 0029908370     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.12.4529-4535.1996     Document Type: Article

References (48)

1. Benz, R., G. Jung, and H.-G. Sahl. 1991. Mechanism of channel formation by lantibiotics in black lipid membranes, p. 359-372. In G. Jung and H.-G. Sahl (ed.), Nisin and novel lantibiotics. Escom Publishers, Leiden, The Netherlands.
2. Bhunia, A. K., M. C. Johnson, and B. Ray. 1987. Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J. Ind. Microbiol. 2:319-322.
3. Bradford, M. M. 1976. A rapid and sensitive method tor the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Bruno, M. E. C., A. Kaiser, and T. J. Montville. 1992. Depletion of proton motive force by nisin in Listeria monocytogenes cells. Appl. Environ. Microbiol. 58:2255-2259.
5. Bruno, M. E. C., and T. J. Montville. 1993. Common mechanism of bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 59:3003-3010.
6. Chen, H.-C., J. H. Brown, J. L. Morell, and C. M. Huang. 1988. Synthetic magainin analogs with improved antimicrobial activity. FEBS Lett. 236:462-466.
7. Chen, Y., and T. J. Montville. 1995. Efflux of ions and ATP depletion induced by pediocin PA-1 are concomitant with cell death in Listeria monocytogenes Scott A. J. Appl. Bacteriol. 79:684-690.
8. Cruciani, R. A., J. L. Barker, S. R. Durell, G. Raghunathan, H. R. Guy, M. Zasloff, and E. F. Stanley. 1992. Magainin 2, a natural antibiotic from frog skin, forms ion channels in lipid bilayer membmaes. Eur. J. Pharmacol. 226:287-296.
9. Driessen, A. J. M., H. W. van den Hooven, W. Kuiper, M. van de Kamp, H.-G. Sahl, R. N. H. Konings, and W. N. Konings. 1995. Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles. Biochemistry 34:1606-1614.
10. Foegeding, P. M., A. B. Thomas, D. H. Pilkington, and T. R. Klaenhammer. 1992. Enhanced control of Listeria monocytogenes by in situ-produced pediocin during dry fermented sausage production. Appl. Environ. Microbiol. 58:884-890.
11. Gao, F. H., T. Abee, and W. N. Konings. 1991. Mechanism of action of the peptide antibiotic nisin in liposomes and cytochrome c oxidase-containing proteoliposomes. Appl. Environ. Microbiol. 57:2164-2170.
12. Garcia-Garcera, M. J., M. G. L. Elfernik, J. M. Driessen, and W. N. Konings. 1993. In vitro pore-forming activity of the lantibiotic nisin. Role of protonmotive force and lipid composition. Eur. J. Biochem. 212:417-422.
13. Garver, K. I., and P. M. Muriana. 1993. Detection, identification and characterization of bacteriocin producing lactic acid bacteria from retail food products. Int. J. Food Microbiol. 19:241-258.
14. Garver, K. I., and P. M. Muriana. 1994. Purification and partial amino acid sequence of curvaticin FS47, a heat-stable bacteriocin produced by Lactobacillus curvatus FS47. Appl. Environ. Microbiol. 60:2191-2195.
15. Gazit, E., A. Boman, H. G. Boman, and Y. Shai. 1995. Interaction of the mammalian antimicrobial peptide cecropin P1 with phospholipid vesicles. Biochemistry 34:11479-11488.
16. Grant E., T. J. Beeler, K. M. P. Taylor, K. Gable, and M. A. Roseman. 1992. Mechanism of magainin 2a induced permeabilization of phospholipid vesicles. Biochemistry 31:9912-9918.
17. Hastings, J. W., M. Sailor, K. Johnson, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1991. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J. Bacteriol. 173:7491-7500.
18. He, K., S. J. Ludtke, and H. W. Huang. 1995. Antimicrobial peptide pores in membranes detected by neutron in-plane scattering. Biochemistry 34:15614-15618.
19. Héchard, Y., B. Derijard, F. Letellier, and Y. Cenatiempo. 1992. Characterization and purification of mesentericin Y105, an anti-listeria bacteriocin from Leuconostoc mesenteroides. J. Gen. Microbiol. 138:2725-2731.
20. Henderson, J. T., A. L. Chopko, and P. D. van Wassenaar. 1992. Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0. Arch. Biochem. Biophys. 295:5-12.
21. Holck, A., L. Axelsson, S.-E. Birkeland, T. Aukrust, and H. Bloom. 1992. Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake LB 706. J. Gen. Microbiol. 138:2715-2720.
22. Holo, H., O. Nilssen, and I. F. Nes. 1991. Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene. J. Bacteriol. 173:3879-3887.
23. Huang, H. W., and Y. Wu. 1991. Lipid-alamethicin interactions influence alamethicin orientation. Biophys. J. 60:1079-1087.
24. Jack, R. W., J. R. Tagg, and B. Ray. 1995. Bacteriocins of gram-positive bacteria. Microbiol. Rev. 59:171-200.
25. Kagermeier-Calloway, A. S., and E. Lauer. 1995. Lactobacillus sake Katagiri, Kitahara and Fukami 1934 is the senior synonym for Lactobacillus bavaricus Stetter and Stetter 1980. Int. J. Syst. Bacteriol. 45:398-399.
26. Kaiser, A. L., and T. J. Montville. 1993. The influence of pH and growth rate on production of the bacteriocin, bavaricin MN, in batch and continuous fermentations. J. Appl. Bacteriol. 75:536-540.
27. Klaenhammer, T. R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbio. Rev. 12:39-86.
28. Lambert, J., E. Keppi, J. L. Dimarcq, C. Wicker, J. M. Reichhart, B. Dunbar, P. Lepage, A. Van Dorsselaer, J. Hoffmann, and J. Fothergill. 1989. Insect immunity: isolation from immune blood of the dipteran Phormia terranovae of two insect antibacterial peptides with sequance homology to rabbit lung macrophage bactericidal peptides. Proc. Natl. Acad. Sci. USA 86:262-266.
29. Larsen, A. G., F. K. Vogensen, and J. Josephsen. 1993. Antimicrobial activity of lactic acid bacteria isolated from sour doughs: purification and characterization of bavarian A, a bacteriocin produced by Lactobacillus bavaricus MI401. J. Appl. Bacteriol. 75:113-122.
30. Lehrer, R. I., T. Ganz, and M. E. Selsted. 1991. Defensins: endogenous antibiotic peptides of animal cells. Cell 64:229-230.
31. Lewus, C. B., A. Kaiser, and T. J. Montville. 1991. Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Appl. Environ. Microbiol. 57:1683-1688.
32. Ludtke, S. L., K. He, Y. Wu, and H. W. Huang. 1994. Cooperative membrane insertion of magainin correlated with its cytolytic activity. Biochim. Biophys. Acta 1190:181-184.
33. Montville, T. J., K. Winkowski, and R. D. Ludescher. 1995. Models and mechanisms for bacteriocin action and application. Int. Dairy J. 5:797-814.
34. Mortvedt, C. I., J. Nissen-Meyer, K. Sletten, and I. F. Nes. 1991. Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake LA5. Appl. Environ. Microbiol. 57:1829-1834.
35. Muriana, P. M., and T. R. Klaenhammer. 1991. Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp. J. Bacteriol. 173:1779-1788.
36. New, R. R. C. 1992. Characterization of liposomes, p. 134. In R. R. C. New (ed.), Liposomes, a practical approach. IRL Press, Oxford.
37. Nissen-Meyer, J., H. Holo, L. S. Havarstein, K. Sletten, and I. F. Nes. 1992. A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides. J. Bacteriol. 174:5686-5692.
38. Ojcious, D. M., and J. D. E. Young. 1991. Cytolytic pore forming proteins and peptides: is there a common structural motif? Trend. Biochem. Sci. 16:225-229.
39. Pattus, F., M. C. Martinez, B. Dargent, D. Cavard, R. Verger, and C. Lazdunski. 1983. Interaction of colicin A with phospholipid monolayers and liposomes. Biochemistry 22:5698-5703.
40. Quadri, L. E. N., M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1994. Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV 17B. J. Biol. Chem. 269:12204-12211.
41. Schägger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
42. Teeter, M. M., J. A. Mazer, and J. J. L'Italien. 1981. Primary structure of the hydrophobic plant protein crambin. Biochemistry 20:5437-5443.
43. Tichaczek, P. S., R. F. Vogel, and W. P. Hammes. 1994. Cloning and sequencing of sakP encoding sakacin P, the bacteriocin produced by Lactobacillus sake LTH 673. Microbiology 140:361-367.
44. van Belkum, M. J., K. Kok, G. Venema, H. Holo, I. F. Nes, W. N. Konings, and T. Abee. 1991. The bacteriocin lactococcin A specifically increases permeability of lactococcal cytoplasmic membranes in a voltage-independent, protein-mediated manner. J. Bacteriol. 173:7934-7941.
45. Vernon, L. P., G. E. Evett, R. D. Zeikus, and W. R. Gray. 1985. A toxic thionin from Pyrularia pubera: purification, properties and amino acid sequence. Arch. Biochem. Biophys. 238:18-29.
46. Winkowski, K. 1996. Mechanistic action of the antimicrobial peptide nisin. Ph.D. thesis. Rutgers, The State University of New Jersey, New Brunswick.
47. Winkowski, K., R. D. Ludescher, and T. J. Montville. 1996. Physicochemical characterization of the nisin-membrane interaction using liposomes derived from Listeria monocytogenes. Appl. Environ. Microbiol. 62:323-327.
48. Zadjel, J. L., P. Ceglowski, and W. T. Dobrzanski. 1985. Mechanism of action of lactostrepcin 5, a bacteriocin produced by Streptococcus cremoris 202. Appl. Environ. Microbiol. 49:969-974.