Transfer of engineered biophysical properties between different antibody formats and expression systems

Protein Engineering, Design and Selection

Volumn 25, Issue 10, 2012, Pages 485-505

  Schaefer, Jonas V ^a   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

antibody engineering; denaturation; IgG; Pichia pastoris; protein stability

Indexed keywords

ANTIBODY ENGINEERING; ANTIBODY FRAGMENT; BIOPHYSICAL PROPERTIES; CONSENSUS ALIGNMENT; DISULFIDE BONDS; EXPRESSION LEVELS; EXPRESSION SYSTEM; FAB FRAGMENTS; FUNCTIONAL EXPRESSION; IGG; IMMUNOGLOBULIN G; MAMMALIAN CELLS; PERIPLASM; PICHIA PASTORIS; PROTEIN ENGINEERING; PROTEIN STABILITY; RECOMBINANT ANTIBODY; SINGLE-CHAIN FV; STABILITY IMPROVEMENT; STRUCTURAL HOMOGENEITY; STRUCTURE-BASED; VARIABLE DOMAIN;

COVALENT BONDS; DENATURATION; ESCHERICHIA COLI; GENETIC ENGINEERING; OLIGOMERS; STABILITY; YEAST;

ANTIBODIES;

IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY ENGINEERING; ARTICLE; CAPILLARY ELECTROPHORESIS; CIRCULAR DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; DISULFIDE BOND; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROKARYOTE; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN STABILITY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; THERMOSTABILITY;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; MOLECULAR SEQUENCE DATA; MUTATION; PICHIA; PROTEIN ENGINEERING; PROTEIN STABILITY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT;

ESCHERICHIA COLI; MAMMALIA; PICHIA PASTORIS; PROKARYOTA;

EID: 84867024410     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs039     Document Type: Article

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