Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies

Metabolic Engineering

Volumn 13, Issue 2, 2011, Pages 241-251

  Makino, Tomohiro ^a,b,d   Skretas, Georgios ^a,b,c   Kang, Tae Hyun ^a   Georgiou, George ^a,b,d  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^c INSTITUTE OF BIOLOGY   (Greece)

^d DAIICHI SANKYO CO   (Japan)

Author keywords

Chemical mutagenesis; Escherichia coli; Fluorescence activated cell sorting (FACS); IgG expression; Strain optimization; Translational initiation region (TIR)

Indexed keywords

CHEMICAL MUTAGENESIS; FLUORESCENCE-ACTIVATED CELL SORTING (FACS); IGG EXPRESSION; STRAIN OPTIMIZATION; TRANSLATIONAL INITIATION;

ANTIBODIES; CELL CULTURE; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; OPTIMIZATION; STRAIN;

MUTAGENESIS;

CHAPERONE; IMMUNOGLOBULIN G ANTIBODY;

ARTICLE; CISTRON; CODON; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HEAVY CHAIN; HIGH THROUGHPUT SCREENING; IMMUNOGLOBULIN PRODUCTION; LIGHT CHAIN; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FOLDING; PROTEIN SYNTHESIS; RANDOMIZED CONTROLLED TRIAL;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENGINEERING; HIGH-THROUGHPUT SCREENING ASSAYS; IMMUNOGLOBULIN G; MUTAGENESIS; PROTEIN ENGINEERING;

ESCHERICHIA COLI;

EID: 79952101952     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2010.11.002     Document Type: Article

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