Role of the general stress response during strong overexpression of a heterologous gene in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 58, Issue 3, 2002, Pages 330-337

  Schweder, T ^a,c   Lin, H ^b,c   Jurgen B ^a,c   Breitenstein, A ^b,c   Riemschneider, S ^b,c   Khalameyzer, V ^b,c   Gupta, A ^b,c   Buttner K ^a,c   Neubauer, P ^b,c  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^c UNIVERSITY OF OULU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA GLUCOSIDASE; GENE PRODUCT; MESSENGER RNA; SIGMA FACTOR;

BACTERIUM;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE MUTATION; NONHUMAN; PROTEIN SYNTHESIS; RECOMBINANT GENE; STRESS;

ALPHA-GLUCOSIDASES; BACTERIAL PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; HSP70 HEAT-SHOCK PROTEINS; RECOMBINANT PROTEINS; SIGMA FACTOR;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036186218     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-001-0904-5     Document Type: Article

References (39)

1. Impact of plasmid presence and induction on cellular responses in fed-batch cultures of Escherichia coli
2. 32 by the phage lambda cIII protein
3. High-level expression of recombinant genes in Escherichia coli is dependent on the availability of thednaY gene product
4. A procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polyimin P precipitation and DNA cellulose chromatography
5. Genetic manipulation of stationary-phase genes to enhance recombinant protein production in Escherichia coli
6. Improvement in recombinant protein production in ppGpp-deficient Escherichia coli
7. Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction
8. Negative regulation by RpoS: A case of sigma factor competition
9. S is positively regulated by ppGpp
10. Physiological effects of TGFα-PE40 expression in recombinant E. coli JM109
11. A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli
12. Genomic analysis of high-cell-density recombinant Escherichia coli fermentation and "cell conditioning" for improved recombinant protein yield
13. Production of abnormal proteins in Escherichia coli stimulates transcription of lon and other heat shock genes
14. Osmotic regulation of rpoS-dependent genes in Escherichia coli
15. Enzymes: Laboratory-scale production
16. Diverse effects of the MalE-LacZ hybrid protein on Escherichia coli cell physiology
17. 38
18. Regulation of RNA polymerase subunit synthesis in Escherichia coli: Intracellular levels of four species of sigma subunit under various growth conditions
19. Monitoring of genes that respond to overproduction of an insoluble recombinant protein in Escherichia coli glucose-limited fed-batch fermentations
20. Control of formation of active soluble or inactive insoluble baker's yeast alpha-glucosidase PI in Escherichia coli by induction and growth conditions
21. Temperature and induction effects on the degradation rate of an abnormal β-galactosidase in Escherichia coli
22. Structural characteristics of an abnormal protein influencing its proteolytic susceptibility
23. Proteolytic response to the expression of an abnormal β-galactosidase in Escherichia coli
24. RpoS-dependent promoters require guanosine tetraphosphate for induction even in the presence of high levels of sigma(s)
25. s subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability
26. S-dependent stationary phase-induced and osmotically regulated osmY (csi-5) gene suggests novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex and integration host factor in the stationary phase response of Escherichia coli
27. Influence of controlled glucose oscillations on a fed-batch process of recombinant Escherichia coli
28. Cellular responses to strong overexpression of recombinant genes in Escherichia coli DNA relaxation and cell death after induction of α-glucosidase
29. Determination of the maximum specific uptake capacities for glucose and oxygen in glucose limited fed-batch cultivations of Escherichia coli
30. Expression and fermentation strategies for recombinant protein production in Escherichia coli
31. Response of guanosine tetraphosphate to glucose fluctuations in fed-batch cultivations of Escherichia coli
32. Induction of a heat shock-like response by unfolded protein in Escherichia coli: Dependence on protein level not protein degradation
33. Synthesis rates of cellular proteins involved in translation and protein folding are strongly altered in response to overproduction of basic fibroblast growth factor by recombinant Escherichia coli
34. s) by ClpXP protease
35. Stability of urogastone and some fusion derivatives and the induction of stress proteins in Escherichia coli
36. Amplification of ColE1 related plasmids in recombinant cultures of Escherichia coli after IPTG induction
37. S and ppGpp in glucose limited fed-batch and continuous cultures of Escherichia coli
38. Synthesis of proteins in Escherichia coli is limited by the concentration of free ribosomes: Expression from reporter genes does not always reflect functional mRNA levels