Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production: 1. Readjustment of metabolic enzyme synthesis

Biotechnology and Bioengineering

Volumn 80, Issue 3, 2002, Pages 313-319

  Hoffmann, Frank ^a,b   Weber, Jan ^a,c   Rinas, Ursula ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^c F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

Escherichia coli; Metabolic burden; Recombinant protein; Tricarboxylic acid cycle enzymes; Two dimensional gel electrophoresis

Indexed keywords

BIOMASS; BIOSYNTHESIS; ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; METABOLISM;

GROWTH FACTORS;

BIOTECHNOLOGY;

BASIC FIBROBLAST GROWTH FACTOR; ELONGATION FACTOR; ENZYME; OXIDOREDUCTASE; PYRUVATE DEHYDROGENASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL METABOLISM; BIOMASS; BIOSYNTHESIS; CATABOLISM; CITRIC ACID CYCLE; ENERGY TRANSFER; ENZYME SYNTHESIS; ESCHERICHIA COLI; GROWTH RATE; PROTEIN SYNTHESIS; TEMPERATURE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

CITRIC ACID CYCLE; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTOR 2; GENE EXPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; ISOCITRATE DEHYDROGENASE; MALATE DEHYDROGENASE; MODELS, BIOLOGICAL; OXYGEN; PLASMIDS; RECOMBINANT PROTEINS; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; VECTORS;

EID: 0037027382     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10379     Document Type: Article

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