Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger

Bioprocess and Biosystems Engineering

Volumn 30, Issue 6, 2007, Pages 397-418

  Gheshlaghi, R ^a   Scharer, J M ^a   Moo Young, M ^a   Douglas, P L ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Aspergillus niger; Hen egg white lysozyme; Logarithmic sensitivity; Metabolic flux analysis; Metabolic pathways

Indexed keywords

AMINO ACIDS; BIOMASS; CARBOHYDRATES; FERMENTATION; GROWTH RATE; METABOLISM;

ASPERGILLUS NIGER; FED-BATCH FERMENTATIONS; METABOLIC FLUX ANALYSIS; TYROSINE;

FUNGI;

ALANINE; AMINO ACID; CARBOXYLIC ACID; GLUTAMIC ACID; PROLINE; RECOMBINANT PROTEIN; TYROSINE;

ARTICLE; ASPERGILLUS NIGER; BATCH FERMENTATION; BIOMASS; CATABOLISM; FED BATCH FERMENTATION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS;

AMINO ACIDS; ASPERGILLUS NIGER; BIOREACTORS; FERMENTATION; KINETICS; MATHEMATICS; MODELS, BIOLOGICAL; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SYSTEMS BIOLOGY;

ASPERGILLUS NIGER;

EID: 35348887734     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-007-0136-x     Document Type: Article

References (57)

1. Archer DB, Jeens DJ, Mackenzie DA, Brightwell G, Lambert N, Lowe G, Radford SE, Dobson CM (1990) Hen egg white lysozyme expressed in, and secreted form, Aspergillus niger is correctly processed and folded. Bio/Technology 8:741-745
2. Wiebe MG, Karandikar A, Robson GD, Trinci APJ, Canida JLF, Trappe S, Wallis G, Rinase U, Derkx PKF, Madrid SM, Sinniega H, Faus I, Montijn R, Van den Hondel CAMJJ, Punt PJ (2001) Production of tissue plasminogen activator (t-PA) in Aspergillus niger. Biotechnol Bioeng 76:164-174
3. Ward PP, Lo JY, Duke M, May GS, Headon DR, Conneely OM (1992) Production of biologically active recombinant human lactoferrin in Aspergillus oryzae. Biotechnology 10:784-789
4. Gyamerah M, Merichetti G, Adedayo O, Scharer JM, Moo-Young M (2002) Bioprocessing strategies for improving hen egg-white lysozyme (HEWL) production by recombinant Aspergillus niger HEWL WT-13-16. Appl Microbiol Biotechnol 60:403-407
5. Bartsch S, Schimek C, Wostemeyer J (2002) Microprojectile bombardment as a reliable method for transformation of the mucoralean fungus Absidia glauca. Mycoscience 43:213-217
6. Gheshlaghi R, Scharer JM, Moo-Young M, Douglas PL (2005) Medium optimization for hen egg white lysozyme production by recombinant Aspergillus niger using statistical methods. Biotechnol Bioeng 90:754-760
7. Li C, Bai J, Cai Z, Ouyang F (2002) Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. J Biotechnol 93:27-34
8. Alvarez-Vasquez F, Gonzalez-Alcon C, Torres NV (2000) Metabolism of citric acid production by Aspergillus niger: Model definition, steady-state analysis and constrained optimization of citric acid production rate. Biotechnol Bioeng 70:82-108
9. Vallino JJ, Stephanopoulos GN (1993) Metabolic flux distribution in Corynebacterium glutamicum during growth and Lysine overproduction. Biotechnol Bioeng 41:633-646
10. Tacak S, Galik G, Mavituna F, Dervakos G (1998) Metabolic flux distribution for the optimized production of L-glutamate. Enzyme Microb Technol 23:286-300
11. van Gulik WM, de Laat TAM, Vinke JL, Heijnen JJ (2000) Application of metabolic flux analysis for the identification of metabolic bottlenecks in the biosynthesis of Penicillin-G. Biotechnol Bioeng 68:602-618
12. Naeimpoor F, Mavituna F (2000) Metabolic flux analysis in Streptomyces coelicolor under various nutrient limitations. Metab Eng 2:140-148
13. KEGG: http://www.kegg.com
14. BRENDA: http://www.brenda.uni-koeln.de/
15. Pedersen H, Carlsen M, Nielsen J (1999) Identification of enzymes and quantification of metabolic fluxes in the wild type and in a recombinant Aspergillus oryzae strain. Appl Environ Microbiol 65:11-19
16. Arora DK, Elander RP, Mukerji KG (1992) Handbook of applied mycology, fungal biotechnology (V.4)
17. Martinelli SD, Kinghorn JR (1994) Aspergillus: 50 years on. Prog Ind Microbiol 29:61-140
18. Gow NAR and Gadd GM (1995) The growing fungus. Chapman& Hall, UK, pp 211-238
19. Smith JE, Biol FI, Berry DR (1976) The filamentous fungi, vol 2. Biosynthesis and metabolism. Edward Arnold, London
20. David H, Akesson M, Nielsen J (2003) Reconstruction of the central carbon metabolism of Aspergillus niger. Eur J Biochem 270:4243-4253
21. Schuurink R, Busink R, Hondmann DH, Witteveen CF, Visser J (1990) Purification and properties of NADP-dependent glycerol dehydrogenase from Aspergillus nidulans and A. niger. J Gen Microbiol 136:1043-1050
22. Tzagoloff A (1982) Mitochondria. Plenum, New York, pp 199-233
23. Walker JE (1992) The mitochondrial transporter family. Curr Opin Struct Biol 2:519-526
24. Davis RH (1986) Compartmental and regulatory mechanisms in the arginine pathways of Neurospora crassa and Saccharomyces cerecisiae. Microbiol Rev 50:280-313
25. Lejohn HB (1971) Enzyme regulation, lysine pathways and cell wall structures as indicators of major lines of evolution in fungi. Nature 231:164-168
26. Zabriskie TM and Jackson MD (2000) Lysine biosynthesis and metabolism in fungi. Nat Prod Rep 17: 85-97
27. Zubay G (1988) Biochemistry, 2nd edn. Macmillan, New York
28. Morzycka E and Paszewski A (1982) Cysteine and homocysteine synthesis in Sacchoromycopsis lipolytica. Identification and characterization of two cysteine synthases. Acta Biochim Pol 29:81-93
29. Cove DJ (1966) The induction and repression on nitrate reductase in the fungus Aspergillus nidulans. Biochem Biophys Acta 113:51-56
30. Archer DB, MacKenzie DA, Ridout MJ (1995) Heterologous protein secretion by Aspergillus niger growing in submerged culture as dispersed or aggregated mycelia. Appl Microbiol Biotechnol 44:157-160
31. Mackenzie DA, Gendron LCG, Jeenes DJ, Archer DB (1994) Physiological optimization of secreted protein production by Aspergillus niger. Enz Microb Technol 16:276-280
32. Mainwaring DO, Weibe MG, Robson GD, Goldrick M, Jeens DJ, Archer DB, Trinci APJ (1999) Effect of pH on hen egg white lysozyme production and evolution of a recombinant strain of Aspergillus niger. J Biotechnol 75:1-10
33. Gawthray GR (2003) An improved reversed-phase liquid chromatographic method for the analysis of low-molecular mass organic acids in plant root exudates. J Chromatogr A 1011:233-240
34. Bidlingmeyer BA, Cohen SA, Tarvin TL (1984) Rapid analysis of amino acids, using precolumn derivatization. J Chromatogr 336:93-104
35. Gheshlaghi R (2007) Optimization of recombinant protein production by a fungal host. PhD thesis, University of Waterloo, Canada.
36. Blumenthal HJ (1968) Glucose catabolism in fungi. Wallerstein Lab Commun 31(106):171-191
37. Lakshminarayana K, Modi VV, Shah VK (1969) Studies of gluconate metabolism in Aspergillus niger. Arch Mikrobiol 66:396-405
38. Purohit HJ and Ratledge C (1988) Mitochondrial location of pyruvate carboxylase in Aspergillus niger. FEMS Microbiol Lett 55:129-132
39. Bercovitz A, Peleg Y, Battat E, Rokem JS, Goldberg I (1990) Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains. Appl Environ Microbiol 56:1594-1597
40. Nielsen J (1997) Physiological engineering aspects of Penicillium chrysogenum. World Scientific, Singapore, pp 61-138
41. Stephanopoulos G, Aristidou AA, Nielsen J (1998) Metabolic engineering: principles and methodologies. Academic, San Diego, pp 21-79; 309-351
42. Clarke BL (1980) Stability of complex reaction networks. Adv Chem Phys 43:1-215
43. Dantzig GB, Thapa MN (1997) Linear programming 1: Introduction (Springer series in operations research and financial engineering). Springer, New York
44. Lee SY, Papoutsakis ET (1999) Metabolic engineering. In: Edwards JS, Ramakrishna R, Schilling CH, Palsson BO (eds) Metabolic flux balance analysis. Marcel Dekker, New York, pp 13-58
45. Schilling CH, Letscher D, Palsson BO (2000) Theory for the systemic definition of metabolic pathways and their use in interpreting metabolic function from a pathway-oriented perspective. J Theor Biol 203:229-248
46. Brooke A, Kendrick D, Meeraus A, Raman R (1998) "GAMS: A User's Guide," GAMS Development Corporation, USA
47. Hillier FS, Lieberman GJ (2001) Introduction to operations research, seventh edition. McGraw-Hill, New York, pp 230-284
48. Pannell DJ (1997) Introduction to practical linear programming. Wiley, New York, pp 48-72
49. Torres NV, Voit EO (2002) Pathway analysis and optimization in metabolic engineering. Cambridge University Press, pp 42-73
50. Varma A, Palsson BO (1993) Metabolic capabilities of Escherichia coli II. Optimal growth patterns. J Theor Biol 165:503-522
51. Osmani SA, Scrutton C (1983) The sub-cellular localization of pyruvate carboxylase and of some other enzymes in Aspergillus nidulans. Eur J Biochem 133:551-560
52. Jorgensen H, Nielsen J, Villadsen J (1995) Metabolic flux distribution in Peniclillium chrysogenum during fed-batch cultivations. Biotechnol Bioeng 46:117-131
53. Obanye AIC, Hobbs G, Gardner DCJ, Oliver SG (1996) Correlation between carbon flux through the pentose phosphate pathway and production of the antibiotic methylenomycin in Streptomyces coelicolor A3(2). Microbiology 142:133-137
54. dos Santos M, Thygesen G, Kotter P, Olsson L, Nielsen J (2003) Aerobic physiology of redox-engineered Saccharomyces cerevisiae strains modified in the ammonium assimilation for increased NADPH availability. FEMS Yeast Res 4:59-68
55. Wynn JP, bin Abdul Hamid A, Ratledge C (1999) The role of malic enzyme in the regulation of lipid accumulation in filamentous fungi. Microbiology 145:1911-1917
56. 13 C tracer experiments and metabolite balancing for metabolic flux analysis. Comparing two approaches. Biotechnol Bioeng 58:254-257
57. Ruijter GJG, Van de Vondervoort PJI, Visser J (1999) Oxalic acid production by Aspergillus niger: an oxalate-non-producing mutant produces citric acid at pH 5 and in the presence of manganese. Microbiology 145:2569-2576