Metabolic flux analysis of recombinant protein overproduction in Escherichia coli

Biochemical Engineering Journal

Volumn 22, Issue 2, 2005, Pages 167-195

  Özkan, Pinar ^a   Sariyar, Berna ^a   Ütkür, F Özde ^a   Akman, Uǧur ^a   Hortaçsu, Amable ^a  

^a BOGAZICI UNIVERSITY   (Turkey)

Author keywords

Acetic acid; Batch processing; Escherichia coli; Metabolic flux analysis; Microbial growth; Recombinant DNA

Indexed keywords

CATABOLIC PATHWAYS; METABOLIC FLUX ANALYSIS (MFA); PROTEIN SYNTHESIS; RECOMBINANT PROTEINS;

CELL CULTURE; COMPUTATIONAL METHODS; COMPUTER SIMULATION; ESCHERICHIA COLI; GROWTH KINETICS; LINEAR PROGRAMMING; MATHEMATICAL MODELS; METABOLISM; SYNTHESIS (CHEMICAL); VECTORS;

PROTEINS;

ACETIC ACID; BACTERIAL PROTEIN; GLYOXYLIC ACID; HEAT SHOCK PROTEIN; HYBRID PROTEIN; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL METABOLISM; BATCH PROCESS; BIOSYNTHESIS; BIOTECHNOLOGY; CATABOLISM; ESCHERICHIA COLI; EXPRESSION VECTOR; GROWTH RATE; HOST CELL; LINEAR SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN METABOLISM; PROTEIN SYNTHESIS; PROTEIN TRANSPORT;

ESCHERICHIA COLI; VECTORS;

EID: 11144250932     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2004.09.012     Document Type: Article

References (36)

1. F. Baneyx Recombinant protein expression in Escherichia coli Curr. Opin. Biotechnol. 10 1999 411 421
2. B. Glick Metabolic load and heterologous gene expression Biotechnol. Adv. 13 1995 247 261
3. L. Andersson, S. Yang, P. Neubauer, and S.O. Enfors Impact of plasmid presence and induction on cellular responses in fed batch cultures of Escherichia coli J. Biotechnol. 46 1996 255 263
4. W.E. Bentley, N. Mirjalili, D.C. Andersen, R.H. Davis, and D.S. Kompala Plasmid-encoded protein: the principal factor in the metabolic burden associated with recombinant bacteria Biotechnol. Bioeng. 35 1990 668 681
5. C. Tamerler, K. Ulgen, B. Kirdar, and Z.I. Onsan A structured model for intracellular EcoRI endonuclease production by recombinant E. coli 294 Process Biochem. 36 2001 621 627
6. V. Saraswat, D.Y. Kim, J. Lee, and Y.-H. Park Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level FEMS Microbiol. Lett. 179 1999 367 373
7. M.K. Oh, and J.C. Liao DNA microarray detection of metabolic responses to protein overproduction in E. coli Metab. Eng. 2 2000 201 209
8. H.A. George, A.L. Powel, M.E. Dahlgreen, W.K. Herber, R.Z. Maigetter, B.W. Burgess, S.M. Stirdivant, and R.L. Greasham Physiological effects of TGFα-PE40 expression in recombinant Escherichia coli JM109 Biotechnol. Bioeng. 40 2000 437 445
9. F. Hoffmann, J. Weber, and U. Rinas Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production. 1. Readjustment of metabolic enzyme synthesis Biotechnol. Bioeng. 80 2002 313 319
10. A.M. Sanden, I. Prytz, I. Tubulekas, C. Forberg, H. Le, A. Hektor, P. Neubauer, Z. Pragai, C. Harwood, A. Ward, A. Picon, J.T. de Mattos, P. Postma, A. Farewell, T. Nystrom, S. Reeh, S. Pedersen, and G. Larsson Limiting factors in Escherichia coli fed-batch production of recombinant proteins Biotechnol. Bioeng. 81 2003 158 166
11. M. Cseryan-Pushman, W. Kramer, E. Duerschmid, G. Striedner, and K. Bayer Metabolic approaches for the optimization of recombinant fermentation processes Appl. Microbiol. Biotechnol. 53 1999 43 50
12. R.T Gill, J.J. Valdes, and W.E. Bentley A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli Metab. Eng. 2 2000 178 189
13. B. Jürgen, H.Y. Lin, S. Riemschneider, C. Scharf, P. Neubauer, R. Schmid, M. Hecker, and T. Schweder Monitoring of genes that respond to overproduction of an insoluble recombinant protein in Escherichia coli glucose-limited fed-batch fermentations Biotechnol. Bioeng. 70 2000 217 224
14. J. Weber, F. Hofmann, and U. Rinas Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production. 2 Redirection of metabolic fluxes Biotechnol. Bioeng. 80 2002 320 330
15. A.K. Gombert, and J. Nielsen Mathematical modelling of metabolism Curr. Opin. Biotechnol. 11 2000 180 186
16. E. Toksoy, P.H. Ozdinler, Z.I. Onsan, and B. Kirdar High level secretion of Taq1 endonuclease by recombinant E. coli Biotechnol. Tech. 13 1999 803 808
17. C. Tamerler, K. Ülgen, B. Kirdar, and Z.İ. Önsan A structural model for intracellular EcoRI endonuclease production by recombinant E. coli 294 Process Biochem. 36 2001 621 627
18. E. Toksoy, P.H. Özdinler, Z.İ. Önsan, and B. Kirdar High level secretion of TaqI restriction endonuclease by recombinant E. coli Biotechnol. Tech. 13 1991 803 808
19. A. Lehmacher, and H. Bisswanger Isolation and characterization of an extremely thermostable d-xylose isomerase from Thermus aquaticus HB 8 J. Gen. Microbiol. 136 1990 679 686
20. B. Sariyar, Cloning of thermostable glucose isomerase of Thermus thermophilus in E. coli, M.S. Thesis, Boǧaziçi University, 1997.
21. C. Lee, L. Bhatnagar, B.C. Saha, Y.-E. Lee, M. Takagi, T. Imanaka, M. Bagdasarian, and J.G. Zeikus Cloning and expression of the Clostridium thermosulfurogenes glucose isomerase gene in E. coli and Bacillus subtilis Appl. Environ. Microbiol. 56 1990 2638 2643
22. Z. Dishe, and E. Borenfreund A new spectrophotometric method for the detection and determination of keto sugars and trioses J. Biol. Chem. 192 1951 583 587
23. G.N. Stephanopoulos, A.A. Aristidou, and J. Nielssen Metabolic Engineering: Principles and Methodologies 1998 Academic Press San Diego, CA
24. J. Pramanik, and J.D. Keasling Stoichiometric model of E. coli metabolism: incorporation of growth rate dependent biomass composition and mechanistic energy requirements Biotechnol. Bioeng. 56 1997 399 421
25. F. Neidhardt Escherichia coli and Salmonella Cellular and Molecular Biology, vol. 1 1996 ASM Press Washington, DC
26. J.S. Edwards, and B. Palsson Metabolic flux balance analysis and the in silico analysis of E. coli-K12 gene deletions BMC Bioinform. 1 2000 1471 2105
27. EcoCyc and MetaCyc databases. http://www.biocyc.org/.
28. Boehringer-Mannheim biochemical pathways database. http://www.expasy.ch/ cgi-bin/show_thumbnails.pl.
29. KEGG Encyclopedia. http://www.genome.ad.jp/kegg/kegg2.html.
30. J.H. Seo, and J.E. Bailey Effects of recombinant plasmid content on growth properties and cloned gene product formation in Escherichia coli Biotechnol. Bioeng. 27 1985 1668 1674
31. S.B. Lee, D.D.Y. Ryu, R. Seigel, and S.H. Park Performance of recombinant fermentation and evaluation of gene expression efficiency for gene product in two-stage continuous culture system Biotechnol. Bioeng. 31 1998 805 820
32. K. Dekker, A. Sugiura, K. Yamagata, K. Sakaguchi, and S. Udaka Efficient production of thermostable Thermus thermophilus xylose isomerase in E. coli and B. brevis Appl. Microbiol. Biotechnol. 36 1992 727 732
33. F. Hoffman, and U. Rinas Online estimation of the metabolic burden resulting from the synthesis of plasmid encoded and heat shock proteins by monitoring respiratory energy generation Biotechnol. Bioeng. 76 2001 333 340
34. J.E. Bailey, and D.F. Ollis Biochemical Engineering Fundamentals 2nd ed. 1986 McGraw-Hill Singapore
35. J. Nielsen, and J. Villadsen Bioreaction Engineering Principles 1994 Plenum Press New York
36. Y. Wei, J.-M. Lee, C. Richmond, F.R. Blattner, J.A. Rafalski, and R.A. LaRossa High-density microarray-mediated gene expression profiling of Escherichia coli J. Bacteriol. 183 2001 545 556