Limiting factors in Escherichia coli fed-batch production of recombinant proteins

Biotechnology and Bioengineering

Volumn 81, Issue 2, 2003, Pages 158-166

  Sandén, Anna Maria ^a   Prytz, Ingela ^a   Tubulekas, Ioannis ^b   Förberg, Cecilia ^b   Le, Ha ^c   Hektor, Andrea ^c   Neubauer, Peter ^c   Pragai, Zoltan ^d   Harwood, Colin ^d   Ward, Alan ^d   Picon, Antonia ^e   De Mattos, Joost Teixeira ^e   Postma, Pieter ^e   Farewell, Anne ^f   Nyström, Thomas ^f   Reeh, Solvejg ^g   Pedersen, Steen ^g   Larsson, Gen ^a  

^a Department of Biotechnology   (Sweden)

^b BIOVITRUM AB   (Sweden)

^c NEWCASTLE UNIVERSITY   (United Kingdom)

^d MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^e UNIVERSITY OF GOTHENBURG   (Sweden)

^f UNIVERSITY OF AMSTERDAM   (Netherlands)

^g UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Acetic acid formation; By product formation; Fed batch; Feed profile; Induction; MRNA; ppGpp; Recombinant protein production; Specific growth rate; Transcription; Translation

Indexed keywords

CULTIVATION; ESCHERICHIA COLI; GLUCOSE; PHYSIOLOGY; RNA; SYNTHESIS (CHEMICAL);

RIBOSOMES;

PROTEINS;

ACETIC ACID; ALANINE; BETA GALACTOSIDASE; GLYCINE; GUANOSINE 3' DIPHOSPHATE 5' DIPHOSPHATE; MESSENGER RNA; RECOMBINANT PROTEIN; RIBOSOME RNA;

ARTICLE; CITRIC ACID CYCLE; ESCHERICHIA COLI; FED BATCH REACTOR; GROWTH RATE; NONHUMAN; PROTEIN INDUCTION; PROTEIN SYNTHESIS; RIBOSOME; RNA TRANSCRIPTION; TRANSLATION REGULATION;

ACETIC ACID; BACTERIAL PROTEINS; BETA-GALACTOSIDASE; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GLUCOSE; KINETICS; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI;

EID: 12244259063     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10457     Document Type: Article

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