X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger

Biochemical Journal

Volumn 434, Issue 1, 2011, Pages 73-82

  Nachon, Florian ^a   Carletti, Eugenie ^a   Wandhammer, Marielle ^a   Nicolet, Yvain ^b   Schopfer, Lawrence M ^c   Masson, Patrick ^a,c   Lockridge, Oksana ^c  

^a INSTITUT DE RECHERCHE BIOMÉDICALE DES ARMÉES   (France)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Acetylcholinesterase; Bioscavenger; Butyrylcholinesterase; Organophosphorus; Protein engineering; Spontaneous reactivation

Indexed keywords

BUFFER; CATALYTIC BIOSCAVENGER; CHOLINESTERASE; ECOTHIOPATE; FLUORIDE; FLUORIDE SODIUM; G117H PROTEIN; HISTIDINE DERIVATIVE; IMIDAZOLE; MUTANT PROTEIN; ORGANOPHOSPHORUS COMPOUND; PHOSPHINE DERIVATIVE; PHOSPHYLSERINE DERIVATIVE; SCAVENGER; SERINE DERIVATIVE; SULFATE; UNCLASSIFIED DRUG; WATER; [ETHYL[[2 [BIS(PROPAN 2 YL)AMINO]ETHYL]SULFANYL](METHYL)PHOSPHINATE];

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; COVALENT BOND; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; HUMAN; NUCLEOPHILIC SUBSTITUTION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEPHOSPHORYLATION; PROTEIN ENGINEERING; PROTEIN HYDROLYSIS; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; PROTEIN TARGETING; X RAY CRYSTALLOGRAPHY;

EID: 79251561846     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20101648     Document Type: Article

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