Does "Butyrylization" of acetylcholinesterase through substitution of the six divergent aromatic amino acids in the active center gorge generate an enzyme mimic of butyrylcholinesterase?

Biochemistry

Volumn 40, Issue 25, 2001, Pages 7433-7445

  Kaplan, D ^a   Ordentlich, A ^a   Barak, D ^a   Ariel, N ^a   Kronman, C ^a   Velan, B ^a   Shafferman, A ^a  

^a ISRAEL INSTITUTE FOR BIOLOGICAL RESEARCH   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC ACTIVE CENTER RESIDUES;

AMINO ACIDS; CARBOXYLIC ACIDS; CATALYSIS; ENZYMES; MOLECULAR DYNAMICS; SUBSTRATES;

BIOCHEMISTRY;

1,5 BIS(4 ALLYLDIMETHYLAMMONIUMPHENYL)PENTAN 3 ONE DIBROMIDE; 3 (N,N,N TRIMETHYLAMMONIO)TRIFLUOROACETOPHENONE; ACETOPHENONE DERIVATIVE; ACETYLCHOLINESTERASE; ACETYLTHIOCHOLINE; BUTYRYLTHIOCHOLINE; CHOLINESTERASE; DECAMETHONIUM; EDROPHONIUM; ENZYME VARIANT; HUPERZINE A; MUTANT PROTEIN; PHENYLALANINE; PHOSPHONIC ACID DERIVATIVE; TACRINE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; ENZYME ACTIVE SITE; ENZYME STRUCTURE; HUMAN; HUMAN CELL; LIGAND BINDING; MOLECULAR DYNAMICS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RESIDUE ANALYSIS; STRUCTURE ANALYSIS; TORPEDO CALIFORNICA;

ACETYLCHOLINESTERASE; AMINO ACID SUBSTITUTION; BENZENAMINIUM, 4,4'-(3-OXO-1,5-PENTANEDIYL)BIS(N,N-DIMETHYL-N-2-PROPENYL-), DIBROMIDE; BINDING SITES; BUTYRATES; BUTYRYLCHOLINESTERASE; CHOLINESTERASE INHIBITORS; DECAMETHONIUM COMPOUNDS; EDROPHONIUM; HUMANS; HYDROLYSIS; KINETICS; LIGANDS; MOLECULAR MIMICRY; MUTAGENESIS, SITE-DIRECTED; ORGANOPHOSPHORUS COMPOUNDS; SUBSTRATE SPECIFICITY; TACRINE;

EID: 0035954374     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi010181x     Document Type: Article

References (71)