Protein engineering of a human enzyme that hydrolyzes V and G nerve agents: Design, construction and characterization

Chemico-Biological Interactions

Volumn 119-120, Issue , 1999, Pages 413-418

  Broomfield, Clarence A ^a,b   Lockridge, Oksana ^a,b   Millard, Charles B ^a,b  

^a US ARMY MEDICAL RESEARCH INSTITUTE OF CHEMICAL DEFENSE   (United States)

^b UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Butyrylcholinesterase; Enzyme engineering; OPA hydrolase; Organophosphorus anticholinesterases; Site directed mutagenesis; Transition state

Indexed keywords

BENZOYLCHOLINE; BUTYRYLTHIOCHOLINE; CHOLINESTERASE; CHOLINESTERASE REACTIVATOR; DYFLOS; ECOTHIOPATE; ORGANOPHOSPHORUS COMPOUND; OXIME DERIVATIVE; PHYSOSTIGMINE; PRALIDOXIME; PYRIDOSTIGMINE; SARIN; SOMAN;

CONFERENCE PAPER; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME KINETICS; ENZYME MODIFICATION; ENZYME STRUCTURE; GENETIC ENGINEERING; HYDROLYSIS; MUTANT; NONHUMAN; SITE DIRECTED MUTAGENESIS;

ANIMALS; BENZOYLCHOLINE; BINDING SITES; BUTYRYLCHOLINESTERASE; BUTYRYLTHIOCHOLINE; CEPHAMYCINS; CHEMICAL WARFARE AGENTS; CHOLINESTERASE INHIBITORS; DRUG DESIGN; ECHOTHIOPHATE IODIDE; HUMANS; HYDROLYSIS; KINETICS; METABOLIC DETOXICATION, DRUG; MUTAGENESIS, SITE-DIRECTED; ORGANOTHIOPHOSPHORUS COMPOUNDS; PHOSPHORIC ACID ESTERS; PROTEIN ENGINEERING; SARIN; SOMAN; TORPEDO;

EID: 0033001689     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-2797(99)00053-8     Document Type: Conference Paper

References (7)

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7. P.-L. Fortier, personal communication.