A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference

Nature Genetics

Volumn 33, Issue 3, 2003, Pages 401-406

  Rubinson, Douglas A ^a   Dillon, Christopher P ^a   Kwiatkowski, Adam V ^a   Sievers, Claudia ^a,b   Yang, Lili ^c   Kopinja, Johnny ^d   Zhang, Mingdi ^d   McManus, Michael T ^a   Gertler, Frank B ^a   Scott, Martin L ^d   Van Parijs, Luk ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

^c CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^d BIOGEN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; GREEN FLUORESCENT PROTEIN; INTERLEUKIN 2 RECEPTOR; RNA; SHORT HAIRPIN RNA; TUMOR SUPPRESSOR PROTEIN; UNCLASSIFIED DRUG; VIRUS VECTOR;

ALLELE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CHIMERA; CONGENIC STRAIN; DENDRITIC CELL; EXPRESSION VECTOR; FLOW CYTOMETRY; GENE CONTROL; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE SEQUENCE; GENE THERAPY; GENETIC ENGINEERING; GENETIC MANIPULATION; GENETIC TRANSDUCTION; LENTIVIRINAE; MAMMAL CELL; MOUSE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROGENY; PROTEIN EXPRESSION; RECOMBINANT DNA TECHNOLOGY; REPORTER GENE; RETROVIRUS; RNA INTERFERENCE; RNA PROCESSING; RNA STRUCTURE; SEQUENCE ANALYSIS; SILENCER GENE; STEM CELL; T LYMPHOCYTE SUBPOPULATION; TRANSCRIPTION REGULATION; TRANSGENE; TRANSGENIC MOUSE; VIRAL GENE DELIVERY SYSTEM; VIRUS TITRATION; VIRUS VECTOR PLL3.7; ZYGOTE;

ANIMALS; ANTIGENS, CD8; BASE SEQUENCE; CD8-POSITIVE T-LYMPHOCYTES; CELLS, CULTURED; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LENTIVIRUS; LUMINESCENT PROTEINS; MICE; MICE, TRANSGENIC; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RECEPTORS, INTERLEUKIN-2; RNA; RNA INTERFERENCE; STEM CELLS;

ANIMALIA; LENTIVIRUS; MAMMALIA; MUS MUSCULUS; UNIDENTIFIED RETROVIRUS; VECTORS;

EID: 0037370498     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/ng1117     Document Type: Article

References (28)

1. Elbashir, S.M. et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498 (2001).
2. Lee, N.S. et al. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20, 500-505 (2002).
3. Brummelkamp, T.R., Bernards, R. & Agami, R. A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550-553 (2002).
4. McManus, M.T., Petersen, C.P., Haines, B.B., Chen, J. & Sharp, P.A. Gene silencing using micro-RNA designed hairpins. RNA 8, 842-850 (2002).
5. Miyagishi, M. & Taira, K. U6 promoter driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20, 497-500 (2002).
6. Paddison, P.J., Caudy, A.A., Bernstein, E., Hannon, G.J. & Conklin, D.S. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16, 948-958 (2002).
7. Paul, C.P., Good, P.D., Winer, I. & Engelke, D.R. Effective expression of small interfering RNA in human cells. Nat. Biotechnol. 20, 505-508 (2002).
8. Sui, G. et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 5515-5520 (2002).
9. Yu, J.Y., DeRuiter, S.L. & Turner, D.L. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 6047-6052 (2002).
10. Brummelkamp, T.R., Bernards, R. & Agami, R. Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell 2, 243-247 (2002).
11. McCaffrey, A.P. et al. RNA interference in adult mice. Nature 418, 38-39 (2002).
12. McManus, M.T. & Sharp, P.A. Gene silencing in mammals by small interfering RNAs. Nat. Rev. Genet. 3, 737-747 (2002).
13. Lois, C., Refaeli, Y., Qin, X.F. & Van Parijs, L. Retroviruses as tools to study the immune system. Curr. Opin. Immunol. 13, 496-504 (2001).
14. Scherr, M. & Eder, M. Gene transfer into hematopoietic stem cells using lentiviral vectors. Curr. Gene Ther. 2, 45-55 (2002).
15. Tuschl, T. Expanding small RNA interference. Nat. Biotechnol. 20, 446-448 (2002).
16. Naldini, L. Lentiviruses as gene transfer agents for delivery to non-dividing cells. Curr. Opin. Biotechnol. 9, 457-463 (1998).
17. Naldini, L. et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263-267 (1996).
18. Pfeifer, A., Ikawa, M., Dayn, Y. & Verma, I.M. Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc. Natl. Acad. Sci. USA 99, 2140-2145 (2002).
19. Lois, C., Hong, E.J., Pease, S., Brown, E.J. & Baltimore, D. Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295, 868-872 (2002).
20. Hogquist, K.A. et al. T cell receptor antagonist peptides induce positive selection. Cell 76, 17-27 (1994).
21. McManus, M.T. et al. Small interfering RNA-mediated gene silencing in T lymphocytes. J. Immunol. 169, 5754-5760 (2002).
22. Strasser, A. et al. The role of bim, a proapoptotic BH3-only member of the Bcl-2 family in cell-death control. Ann. NY Acad. Sci. 917, 541-548 (2000).
23. Willerford, D.M. et al. Interleukin-2 receptor α chain regulates the size and content of the peripheral lymphoid compartment. Immunity 3, 521-530 (1995).
24. Schmidt, E.V., Christoph, G., Zeller, R. & Leder, P. The cytomegalovirus enhancer: a pan-active control element in transgenic mice. Mol. Cell. Biol. 10, 4406-4411 (1990).
25. Gertler, F.B., Niebuhr, K., Reinhard, M., Wehland, J. & Soriano, P. Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics. Cell 87, 227-239 (1996).
26. Chen, J., Lansford, R., Stewart, V., Young, F. & Alt, F.W. RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development. Proc. Natl. Acad. Sci. USA 90, 4528-4532 (1993).
27. Paddison, P.J. & Hannon, G.J. RNA interference: the new somatic cell genetics? Cancer Cell 2, 17-23 (2002).
28. Hannon, G.J. RNA interference. Nature 418, 244-251 (2002).