Expression of intein-tagged fusion protein and its applications in downstream processing

Journal of Chemical Technology and Biotechnology

Volumn 85, Issue 1, 2010, Pages 11-18

  Wang, Lishi ^a,c   Kang, Jung Hye ^a   Kim, Ki Hyung ^a   Lee, E K ^b  

^a Hanyang University   (South Korea)

^b Gachon University College of Medicine   (South Korea)

^c INNER MONGOLIA MEDICAL COLLEGE   (China)

Author keywords

Affinity tag; Intein; Intervening proteins; Protein purification; Protein splicing element; Self cleaving proteins

Indexed keywords

AFFINITY TAG; CONVENTIONAL METHODS; DOWNSTREAM-PROCESSING; EXPRESSION SYSTEM; FURTHER DEVELOPMENT; FUSION PROTEINS; INDUSTRIAL-SCALE PRODUCTION; INTEIN; PROTEIN PURIFICATION; PROTEIN-SPLICING; PURIFICATION PROCEDURES; PURIFICATION SYSTEMS; RECOMBINANT PROTEIN; TARGET PROTEINS;

GENE EXPRESSION; PURIFICATION;

PROTEINS;

HYBRID PROTEIN; INTEIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; AFFINITY LABELING; AMINO ACID SEQUENCE; DOWNSTREAM PROCESSING; GENE EXPRESSION SYSTEM; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN STRUCTURE, FUNCTION AND VARIABILITY; PURIFICATION; SHORT SURVEY;

EID: 75649127236     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.2277     Document Type: Short Survey

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