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1
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0034602984
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Dissecting the chemistry of protein splicing and its applications
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This is a recent and very extensive review of the chemical mechanism of protein splicing and related applications. Included is a historic perspective on how the mechanism was deciphered.
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Noren C.J., Wang J., Perler F.B. Dissecting the chemistry of protein splicing and its applications. Angew Chem Ed Engl. 39:2000;450-466. This is a recent and very extensive review of the chemical mechanism of protein splicing and related applications. Included is a historic perspective on how the mechanism was deciphered.
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(2000)
Angew Chem Ed Engl
, vol.39
, pp. 450-466
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Noren, C.J.1
Wang, J.2
Perler, F.B.3
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2
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0033963173
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InBase, the intein database
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This paper describes InBase, the online intein resource at which includes the most up-to-date list of inteins, their properties and sequences, scientist contact information, and annotated references
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Perler F.B. InBase, the intein database. Nucleic Acids Res. 28:2000;344-345. This paper describes InBase, the online intein resource at http://www.neb.com/neb/inteins.html , which includes the most up-to-date list of inteins, their properties and sequences, scientist contact information, and annotated references.
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(2000)
Nucleic Acids Res
, vol.28
, pp. 344-345
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Perler, F.B.1
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3
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0344351815
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Semisynthesis of cytotoxic proteins using a modified protein splicing element
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Evans T.C., Benner J., Xu M-Q. Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci. 7:1998;2256-2264.
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(1998)
Protein Sci
, vol.7
, pp. 2256-2264
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Evans, T.C.1
Benner, J.2
Xu, M.-Q.3
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4
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0032499752
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Expressed protein ligation: A general method for protein engineering
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Muir T.W., Sondhi D., Cole P.A. Expressed protein ligation: a general method for protein engineering. Proc Natl Acad Sci USA. 95:1998;6705-6710.
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(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 6705-6710
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Muir, T.W.1
Sondhi, D.2
Cole, P.A.3
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5
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0032534048
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Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
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Chong S., Montello G.E., Zhang A., Cantor E.J., Liao W., Xu M.Q., Benner J. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 26:1998;5109-5115.
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(1998)
Nucleic Acids Res
, vol.26
, pp. 5109-5115
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Chong, S.1
Montello, G.E.2
Zhang, A.3
Cantor, E.J.4
Liao, W.5
Xu, M.Q.6
Benner, J.7
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6
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17744415288
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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
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Chong S., Mersha F.B., Comb D.G., Scott M.E., Landry D., Vence L.M., Perler F.B., Benner J., Kucera R.B., Hirvonen C.A.et al. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene. 192:1997;271-281.
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(1997)
Gene
, vol.192
, pp. 271-281
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Chong, S.1
Mersha, F.B.2
Comb, D.G.3
Scott, M.E.4
Landry, D.5
Vence, L.M.6
Perler, F.B.7
Benner, J.8
Kucera, R.B.9
Hirvonen, C.A.10
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7
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0040559903
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The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins
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This paper describes the Twin vector system, which yields proteins or polypeptides with intein-mediated protein ligation (IPL) reactive ends on both sides of the molecule.
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Evans T.C. Jr., Benner J., Xu M.Q. The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins. J Biol Chem. 274:1999;18359-18363. This paper describes the Twin vector system, which yields proteins or polypeptides with intein-mediated protein ligation (IPL) reactive ends on both sides of the molecule.
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(1999)
J Biol Chem
, vol.274
, pp. 18359-18363
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Evans T.C., Jr.1
Benner, J.2
Xu, M.Q.3
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8
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0033614484
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Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- And C-terminal cleavage elements: Facile production of protein building blocks for protein ligation
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Mathys S., Evans T.C., Chute I.C., Wu H., Chong S., Benner J., Liu X.Q., Xu M.Q. Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene. 231:1999;1-13.
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(1999)
Gene
, vol.231
, pp. 1-13
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Mathys, S.1
Evans, T.C.2
Chute, I.C.3
Wu, H.4
Chong, S.5
Benner, J.6
Liu, X.Q.7
Xu, M.Q.8
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9
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0032988320
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Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein
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Southworth M.W., Amaya K., Evans T.C., Xu M.Q., Perler F.B. Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. BioTechniques. 27:1999;110-120.
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(1999)
BioTechniques
, vol.27
, pp. 110-120
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Southworth, M.W.1
Amaya, K.2
Evans, T.C.3
Xu, M.Q.4
Perler, F.B.5
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10
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0032832776
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A genetic system yields self-cleaving inteins for bioseparations
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The intein carboxy-terminal splice junction cleavage vector described in this paper was selected in a novel genetic screen to maximize splice junction cleavage efficiency.
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Wood D.W., Wu W., Belfort G., Derbyshire V., Belfort M. A genetic system yields self-cleaving inteins for bioseparations. Nat Biotechnol. 17:1999;889-892. The intein carboxy-terminal splice junction cleavage vector described in this paper was selected in a novel genetic screen to maximize splice junction cleavage efficiency.
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(1999)
Nat Biotechnol
, vol.17
, pp. 889-892
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Wood, D.W.1
Wu, W.2
Belfort, G.3
Derbyshire, V.4
Belfort, M.5
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11
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0032882026
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Construction and use of low-copy number T7 expression vectors for purification of problem proteins: Purification of Mycobacterium tuberculosis RmlD and Pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI
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Hoang T.T., Ma Y., Stern R.J., McNeil M.R., Schweizer H.P. Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of Mycobacterium tuberculosis RmlD and Pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI. Gene. 237:1999;361-371.
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(1999)
Gene
, vol.237
, pp. 361-371
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Hoang, T.T.1
Ma, Y.2
Stern, R.J.3
McNeil, M.R.4
Schweizer, H.P.5
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12
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0032562685
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Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein
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Chong S., Williams K.S., Wotkowicz C., Xu M.Q. Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J Biol Chem. 273:1998;10 567-10 577.
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(1998)
J Biol Chem
, vol.273
, pp. 10567-10577
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Chong, S.1
Williams, K.S.2
Wotkowicz, C.3
Xu, M.Q.4
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13
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0033038793
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Improved segmental isotope labeling of proteins and application to a larger protein
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Otomo T., Teruya K., Uegaki K., Yamazaki T., Kyogoku Y. Improved segmental isotope labeling of proteins and application to a larger protein. J Biomol NMR. 14:1999;105-114.
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(1999)
J Biomol NMR
, vol.14
, pp. 105-114
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Otomo, T.1
Teruya, K.2
Uegaki, K.3
Yamazaki, T.4
Kyogoku, Y.5
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14
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0033534179
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NMR observation of selected segments in a larger protein: Central-segment isotope labeling through intein-mediated ligation
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This paper, along with [13,18], describes how to use splicing of split inteins for segmental labeling and NMR analysis, including specific labeling of the middle of a protein.
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Otomo T., Ito N., Kyogoku Y., Yamazaki T. NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation. Biochemistry. 38:1999;16040-16044. This paper, along with [13,18], describes how to use splicing of split inteins for segmental labeling and NMR analysis, including specific labeling of the middle of a protein.
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(1999)
Biochemistry
, vol.38
, pp. 16040-16044
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Otomo, T.1
Ito, N.2
Kyogoku, Y.3
Yamazaki, T.4
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15
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0032584098
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Protein splicing in trans by purified N- And C-terminal fragments of the Mycobacterium tuberculosis RecA intein
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Mills K.V., Lew B.M., Jiang S., Paulus H. Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein. Proc Natl Acad Sci USA. 95:1998;3543-3548.
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(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 3543-3548
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Mills, K.V.1
Lew, B.M.2
Jiang, S.3
Paulus, H.4
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16
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18144439565
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Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein
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Wu H., Xu M.Q., Liu X.Q. Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein. Biochim Biophys Acta. 1387:1998;422-432.
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(1998)
Biochim Biophys Acta
, vol.1387
, pp. 422-432
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Wu, H.1
Xu, M.Q.2
Liu, X.Q.3
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17
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0032481347
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Control of protein splicing by intein fragment reassembly
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Southworth M.W., Adam E., Panne D., Byer R., Kautz R., Perler F.B. Control of protein splicing by intein fragment reassembly. EMBO J. 17:1998;918-926.
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(1998)
EMBO J
, vol.17
, pp. 918-926
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Southworth, M.W.1
Adam, E.2
Panne, D.3
Byer, R.4
Kautz, R.5
Perler, F.B.6
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18
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0032503612
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Segmental isotope labeling for protein NMR using peptide splicing
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Yamazaki T., Otomo T., Oda N., Kyogoku Y., Uegaki K., Ito N., Ishino Y., Nakamura H. Segmental isotope labeling for protein NMR using peptide splicing. J Am Chem Soc. 120:1998;5591-5592.
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(1998)
J Am Chem Soc
, vol.120
, pp. 5591-5592
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Yamazaki, T.1
Otomo, T.2
Oda, N.3
Kyogoku, Y.4
Uegaki, K.5
Ito, N.6
Ishino, Y.7
Nakamura, H.8
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19
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0032569012
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Protein splicing in vitro with a semisynthetic two-component minimal intein
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Lew B.M., Mills K.V., Paulus H. Protein splicing in vitro with a semisynthetic two-component minimal intein. J Biol Chem. 273:1998;15 887-15 890.
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(1998)
J Biol Chem
, vol.273
, pp. 15887-15890
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Lew, B.M.1
Mills, K.V.2
Paulus, H.3
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20
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0032483013
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Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803
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Wu H., Hu Z., Liu X.Q. Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proc Natl Acad Sci USA. 95:1998;9226-9231.
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(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 9226-9231
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Wu, H.1
Hu, Z.2
Liu, X.Q.3
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21
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0033143682
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A natural example of protein trans-splicing
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Perler F.B. A natural example of protein trans-splicing. Trends Biochem Sci. 24:1999;209-210.
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(1999)
Trends Biochem Sci
, vol.24
, pp. 209-210
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Perler, F.B.1
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22
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0032579457
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Molecular dissection of the Mycobacterium tuberculosis RecA intein: Design of a minimal intein and of a trans-splicing system involving two intein fragments
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Shingledecker K., Jiang S., Paulus H. Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments. Gene. 207:1998;187-195.
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(1998)
Gene
, vol.207
, pp. 187-195
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Shingledecker, K.1
Jiang, S.2
Paulus, H.3
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23
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0032498234
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Protein splicing of inteins and hedgehog autoproteolysis: Structure, function, and evolution
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Perler F.B. Protein splicing of inteins and hedgehog autoproteolysis: structure, function, and evolution. Cell. 92:1998;1-4.
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(1998)
Cell
, vol.92
, pp. 1-4
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Perler, F.B.1
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24
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0030611387
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Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity
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Duan X., Gimble F.S., Quiocho F.A. Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity. Cell. 89:1997;555-564.
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(1997)
Cell
, vol.89
, pp. 555-564
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Duan, X.1
Gimble, F.S.2
Quiocho, F.A.3
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25
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0030911066
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Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs
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Chong S., Xu M.Q. Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs. J Biol Chem. 272:1997;15 587-15 590.
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(1997)
J Biol Chem
, vol.272
, pp. 15587-15590
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Chong, S.1
Xu, M.Q.2
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26
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0030803760
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Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution
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Derbyshire V., Wood D.W., Wu W., Dansereau J.T., Dalgaard J.Z., Belfort M. Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution. Proc Natl Acad Sci USA. 94:1997;11466-11471.
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(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 11466-11471
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Derbyshire, V.1
Wood, D.W.2
Wu, W.3
Dansereau, J.T.4
Dalgaard, J.Z.5
Belfort, M.6
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27
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0344994534
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NMR spectroscopy of large molecules and multimolecular assemblies in solution
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Wider G., Wuthrich K. NMR spectroscopy of large molecules and multimolecular assemblies in solution. Curr Opin Struct Biol. 9:1999;594-601.
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(1999)
Curr Opin Struct Biol
, vol.9
, pp. 594-601
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Wider, G.1
Wuthrich, K.2
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28
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0033598747
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Production of cyclic peptides and proteins in vivo
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The SICLOPPS method of protein and polypeptide backbone cyclization using split inteins is defined in this paper. The expression of cyclic peptides or peptide libraries in vivo provides tools for identifying inhibitors, activators or binding peptides. Cyclization may improve peptide/protein stability and bioactivity.
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Scott C.P., Abel-Santos E., Wall M., Wahnon D.C., Benkovic S.J. Production of cyclic peptides and proteins in vivo. Proc Natl Acad Sci USA. 96:1999;13 638-13 643. The SICLOPPS method of protein and polypeptide backbone cyclization using split inteins is defined in this paper. The expression of cyclic peptides or peptide libraries in vivo provides tools for identifying inhibitors, activators or binding peptides. Cyclization may improve peptide/protein stability and bioactivity.
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(1999)
Proc Natl Acad Sci USA
, vol.96
, pp. 13638-13643
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Scott, C.P.1
Abel-Santos, E.2
Wall, M.3
Wahnon, D.C.4
Benkovic, S.J.5
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29
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0032724470
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Chemical protein synthesis
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A review of various chemical protein ligation schemes, including intein-mediated protein ligation.
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Kochendoerfer G.G., Kent S.B. Chemical protein synthesis. Curr Opin Chem Biol. 3:1999;665-671. A review of various chemical protein ligation schemes, including intein-mediated protein ligation.
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(1999)
Curr Opin Chem Biol
, vol.3
, pp. 665-671
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Kochendoerfer, G.G.1
Kent, S.B.2
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30
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0005464510
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Native chemical ligation of polypeptides
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An excellent methods paper for intein-mediated protein ligation reactions, although limited by the absence of the newer and more diverse vectors now available.
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Camarero J.A., Muir T.W. Native chemical ligation of polypeptides. Curr Protocols Protein Sci. 18.4:1999;1-21. An excellent methods paper for intein-mediated protein ligation reactions, although limited by the absence of the newer and more diverse vectors now available.
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(1999)
Curr Protocols Protein Sci
, vol.184
, pp. 1-21
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Camarero, J.A.1
Muir, T.W.2
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31
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0033540665
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Insertion of a synthetic peptide into a recombinant protein framework: A protein biosensor
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One of the more exciting applications of IPL is described. The authors introduce an environmentally sensitive fluorophore between two kinase domains. The fluorescence properties of the biosensor are dependent on the functional state of the process examined, such as enzyme activity or ligand binding.
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Cotton G.J., Ayers B., Xu R., Muir T.W. Insertion of a synthetic peptide into a recombinant protein framework: a protein biosensor. J Am Chem Soc. 121:1999;1100-1101. One of the more exciting applications of IPL is described. The authors introduce an environmentally sensitive fluorophore between two kinase domains. The fluorescence properties of the biosensor are dependent on the functional state of the process examined, such as enzyme activity or ligand binding.
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(1999)
J Am Chem Soc
, vol.121
, pp. 1100-1101
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Cotton, G.J.1
Ayers, B.2
Xu, R.3
Muir, T.W.4
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32
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0033582287
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Chemical ligation of folded recombinant proteins: Segmental isotopic labeling of domains for NMR studies
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Xu R., Ayers B., Cowburn D., Muir T.W. Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies. Proc Natl Acad Sci USA. 96:1999;388-393.
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(1999)
Proc Natl Acad Sci USA
, vol.96
, pp. 388-393
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Xu, R.1
Ayers, B.2
Cowburn, D.3
Muir, T.W.4
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33
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0344199948
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Circular beta-lactamase: Stability enhancement by cyclizing the backbone
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Iwai H., Pluckthun A. Circular beta-lactamase: stability enhancement by cyclizing the backbone. FEBS Lett. 459:1999;166-172.
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(1999)
FEBS Lett
, vol.459
, pp. 166-172
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Iwai, H.1
Pluckthun, A.2
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34
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0032568924
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Expressed Protein Ligation, a novel method for studying protein-protein interactions in transcription
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Severinov K., Muir T.W. Expressed Protein Ligation, a novel method for studying protein-protein interactions in transcription. J Biol Chem. 273:1998;16 205-16 209.
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(1998)
J Biol Chem
, vol.273
, pp. 16205-16209
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Severinov, K.1
Muir, T.W.2
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35
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0039374819
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The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum
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Evans T.C. Jr., Benner J., Xu M.Q. The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum. J Biol Chem. 274:1999;3923-3926.
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(1999)
J Biol Chem
, vol.274
, pp. 3923-3926
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Evans T.C., Jr.1
Benner, J.2
Xu, M.Q.3
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36
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0033229860
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Expressed protein ligation to probe regiospecificity of heterocyclization in the peptide antibiotic microcin B17
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Roy R.S., Allen O., Walsh C.T. Expressed protein ligation to probe regiospecificity of heterocyclization in the peptide antibiotic microcin B17. Chem Biol. 6:1999;789-799.
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(1999)
Chem Biol
, vol.6
, pp. 789-799
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Roy, R.S.1
Allen, O.2
Walsh, C.T.3
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37
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0033575092
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Biosynthesis of a head-to-tail cyclized protein with improved biological activity
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Camarero J.A., Muir T.W. Biosynthesis of a head-to-tail cyclized protein with improved biological activity. J Am Chem Soc. 121:1999;5597-5598.
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(1999)
J Am Chem Soc
, vol.121
, pp. 5597-5598
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Camarero, J.A.1
Muir, T.W.2
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38
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0032464350
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Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis
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Kinsland C., Taylor S.V., Kelleher N.L., McLafferty F.W., Begley T.P. Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis. Protein Sci. 7:1998;1839-1842.
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(1998)
Protein Sci
, vol.7
, pp. 1839-1842
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Kinsland, C.1
Taylor, S.V.2
Kelleher, N.L.3
McLafferty, F.W.4
Begley, T.P.5
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39
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0033534783
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Use of benzyl mercaptan for direct preparation of long polypeptide benzylthio esters as substrates of subtiligase
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Welker E., Scheraga H.A. Use of benzyl mercaptan for direct preparation of long polypeptide benzylthio esters as substrates of subtiligase. Biochem Biophys Res Commun. 254:1999;147-151.
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(1999)
Biochem Biophys Res Commun
, vol.254
, pp. 147-151
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Welker, E.1
Scheraga, H.A.2
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40
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0032189519
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Methionine ligation strategy in the biomimetic synthesis of parathyroid hormones
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Tam J.P., Yu Q. Methionine ligation strategy in the biomimetic synthesis of parathyroid hormones. Biopolymers. 46:1998;319-327.
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(1998)
Biopolymers
, vol.46
, pp. 319-327
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Tam, J.P.1
Yu, Q.2
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41
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0032505062
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Identification of a virus intein and a possible variation in the protein-splicing reaction
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Pietrokovski S. Identification of a virus intein and a possible variation in the protein-splicing reaction. Curr Biol. 8:1998;R634-R635.
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(1998)
Curr Biol
, vol.8
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Pietrokovski, S.1
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42
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0034595699
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Structural insights into the protein splicing mechanism of Pl-Scel
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This is the first structure of a precursor with residues flanking both splice junctions and helps to refine the molecular mechanism of protein splicing.
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Poland B.W., Xu M.Q., Quiocho F.A. Structural insights into the protein splicing mechanism of Pl-Scel. J Biol Chem. 275:2000;16408-16413. This is the first structure of a precursor with residues flanking both splice junctions and helps to refine the molecular mechanism of protein splicing.
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(2000)
J Biol Chem
, vol.275
, pp. 16408-16413
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Poland, B.W.1
Xu, M.Q.2
Quiocho, F.A.3
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43
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0031975772
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Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing
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Klabunde T., Sharma S., Telenti A., Jacobs E.R. Jr., Sacchetini J.C. Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nat Struct Biol. 5:1998;31-36.
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(1998)
Nat Struct Biol
, vol.5
, pp. 31-36
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Klabunde, T.1
Sharma, S.2
Telenti, A.3
Jacobs E.R., Jr.4
Sacchetini, J.C.5
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