Simultaneous refolding and purification of a recombinant lipase with an intein tag by affinity precipitation with chitosan

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1784, Issue 11, 2008, Pages 1825-1829

  Singh, Pradeep K ^a   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Affinity precipitation; Intein tag; Protein refolding; Recombinant lipase; Smart polymer

Indexed keywords

BUFFER; CETRIMIDE; CHITIN; CHITOSAN; DITHIOTHREITOL; HYBRID PROTEIN; INTEIN; POLYMER; TRIACYLGLYCEROL LIPASE; UREA;

ARTICLE; BINDING AFFINITY; CELL INCLUSION; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; SOLUBILITY;

BURKHOLDERIA CEPACIA; CHITIN; CHITOSAN; CHROMATOGRAPHY, AFFINITY; EFFICIENCY; INTEINS; LIPASE; PRECIPITATION; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 54049122125     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2008.07.012     Document Type: Article

References (29)

1. Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60 (2003) 523-533
2. Jungbauer A., and Kaar W. Current status of technical protein refolding. J. Biotechnol. 128 (2007) 587-596
3. Chong S., Monntello G.E., Zhang A., Cantor E.J., Liao W., Xu M.Q., and Benner J. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acid Res. 26 (1998) 5109-5115
4. Guo C., Li Z., Shi Y., Xu M., Wise J.G., Trommer W.E., and Yuan J. Intein-mediated fusion expression, high efficient refolding and one-step purification of gelonin toxin. Protein Expr. Purif. 37 (2004) 361-367
5. Przybycien T.M., Pujar N.S., and Steele L.M. Alternative bioseparation operations: life beyond packed-bed chromatography. Curr. Opin. Biotechnol. 15 (2004) 469-478
6. Mondal K., Roy I., and Gupta M.N. Affinity based strategies for protein purification. Anal. Chem. 78 (2006) 3499-3504
7. Senstad C., and Mattiasson B. Purification of wheat germ agglutinin using affinity flocculation with chitosan and a subsequent centrifugation or flotation step. Biotech. Bioeng. 34 (1988) 387-393
8. Teotia S., Lata R., and Gupta M.N. Chitosan as a macro-(affinity ligand): purification of chitinases by affinity precipitation and aqueous two-phase extractions. J. Chromatogr. A. 1052 (2004) 85-91
9. Teotia S., Mondal K., and Gupta M.N. Integration of affinity precipitation with partitioning methods for bioseparation of chitin binding lectins. Food Bioproducts Process. 84 (2006) 1-7
10. Gupta M.N., and Mattiasson B. In: Street G. (Ed). Highly Selective Separation in Biotechnology (1994), Chapmann and Hall, London 7-33
11. Roy I., and Gupta M.N. Smart polymeric materials: emerging biochemical applications. Chem. Biol. 10 (2003) 1161-1171
12. Kuboi R., Morita S., Ota H., and Umakoshi H. Protein refolding using stimuli-responsive polymer-modified aqueous two-phase systems. J. Chromatogr. B. 743 (2000) 215-223
13. Umakoshi H., Persson J., Kroon M., Johansson H.-O., Otzen D.E., Kuboi R., and Tjerneld F. Model process for separation based on unfolding and refolding of chymotrypsin inhibitor 2 in thermoseparating polymer two-phase systems. J. Chromatogr. B 743 (2000) 13-19
14. Roy I., and Gupta M.N. pH-responsive polymer-assisted refolding of urea- and organic solvent-denatured α-chymotrypsin. Protein Eng. 16 (2003) 1153-1157
15. Mondal K., Bohidar H.B., Roy R.P., and Gupta M.N. Alginate-chaperoned facile refolding of Chromobacterium viscosum lipase. Biochim. Biophys. Acta 1764 (2006) 877-886
16. Mondal K., Raghava S., Barua B., Varadarajan R., and Gupta M.N. Role of stimuli-sensitive polymers in protein refolding: α-amylase and CcdB (controller of cell division or death B) as model proteins. Langmuir 23 (2007) 70-75
17. Jain P., Jain S., and Gupta M.N. A microwave assisted microassay for lipase. Anal. Bioanal. Chem. 381 (2005) 1480-1482
18. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72 (1976) 248-254
19. S. Dalal, P.K. Singh, S. Raghava, S. Rawat, M.N. Gupta, Purification and properties of the alkaline lipase from Burkholderia cepacia ATCC 25609, Biotechnol. Appl. Biochem. doi:10.1042/BA20070186.
20. Puri N.K., Crivelli E., Cardamone M., Fiddes R., Bertolini J., Ninham B., and Brandon M.R. Solubilization of growth hormone and other recombinant proteins from Escherichia coli inclusion bodies by using a cationic surfactant. Biochem. J. 285 (1992) 871-879
21. Wang H., and Chong S. Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex. Proc. Natl. Acad. Sci. 100 (2003) 478-483
22. Cornish-Bowden A. Analysis of Enzyme Data (1995), Oxford University Press, Oxford
23. Hames B.D. One-dimensional polyacrylamide gel electrophoresis. In: Hames B.D., and Rickwood D. (Eds). Gel Electrophoresis of Proteins, A Practical Approach. 2nd ed. (1986), IRL press, Oxford 1-86
24. Galluccio M., Brizio C., Torchetti E.M., Ferranti P., Gianazza E., Indiveri C., and Barile M. Over-expression in Escherichia coli, purification and characterization of isoform 2 of human FAD synthetase. Protein Expr. Purif. 52 (2007) 175-181
25. Lakowicz J.R. Principles of Fluorescence Spectroscopy. 2nd ed. (1999), Kluwer Academic/Plenum Publishers, New York 446-486
26. Matsuo K., Yonehara R., and Gekko K. Improved estimation of the secondary structures of proteins by vacuum-ultraviolet circular dichroism spectroscopy. J. Biochem. 138 (2005) 79-88
27. Sharma A., Roy I., and Gupta M.N. Affinity precipitation and macro-(affinity ligand) facilitated three phase partitioning for refolding and simultaneous purification of urea-denatured pectinase. Biotechnol. Prog. 20 (2004) 1255-1256
28. Gupta M.N., and Mattiasson B. Affinity precipitation. In: Street G. (Ed). Highly Selective Separations in Biotechnology (1994), Chapman and Hall, London 7-33
29. Banki M.R., and Wood D.W. Inteins and affinity resin substitutes for protein purification and scale up. Microb. Cell Fact. 4 (2005) 1-6