Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli

Protein Expression and Purification

Volumn 43, Issue 1, 2005, Pages 26-32

  Sun, Ziyong ^a   Chen, Junyong ^a   Yao, Hongwei ^a   Liu, Lili ^a   Wang, Jing ^a   Zhang, Jing ^a   Liu, Jian Ning ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Chitin affinity column; hBNP; Reverse phase HPLC; Ssp dnaB derived mini intein

Indexed keywords

ADENOSINE TRIPHOSPHATASE; BRAIN NATRIURETIC PEPTIDE; DNAB HELICASE; DNAB PROTEIN, E COLI; GREEN FLUORESCENT PROTEIN; HELICASE; HYBRID PROTEIN; INTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENE FUSION; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PROCESSING;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; DNA HELICASES; DNAB HELICASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE FUSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; INTEINS; MOLECULAR SEQUENCE DATA; NATRIURETIC PEPTIDE, BRAIN; PLASMIDS; PROTEIN SPLICING; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

EID: 23244448024     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.05.005     Document Type: Article

References (18)

1. G. Hanning, and S.C. Makrides Strategies for optimizing heterologous protein expression in Escherichia coli Trends Biotechnol. 16 1998 54 60
2. S. Gottesman Genetics of proteolysis in Escherichia coli Annu. Rev. Genet. 23 1989 163 198
3. J. Nilsson, S. Stahl, J. Lundeberg, M. Uhlen, and P.A. Nygren Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins Protein Express. Purif. 11 1997 1 16
4. E.R. Lavallie, and J.M. McCoy Gene fusion expression systems in Escherichia coli Curr. Opin. Biotechnol. 6 1995 501 506
5. E.J. Cantor, and S. Chong Intein-mediated rapid purification of Cre recombinase Protein Express. Purif. 22 2001 135 140
6. P. Lepage, C. Heckel, S. Humbert, S. Stahl, and G. Rautmann Recombinant technology as an alternative to chemical peptide synthesis: expression and characterization of HIV-1 Rev recombinant peptides Anal. Biochem. 213 1993 40 48
7. P.A. Nygren, S. Stahl, and M. Uhlen Engineering proteins to facilitate bioprocessing Trends Biotechnol. 12 1994 184 188
8. S. Chong, G.E. Montello, A. Zhang, E.J. Cantor, W. Liao, M. Xu, and J. Benner Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step Nucleic Acids Res. 26 1998 5109 5115
9. I.R. Cottinghan, A. Millar, E. Emslie, A. Colman, A.E. Schnieke, and C.A. McKee Method for the amidation of recombinant peptides expressed as intein fusion proteins in Escherichia coli Nat. Biotechnol. 19 2001 974 977
10. S. Mathys, T.C. Evans, I.C. Chute, H. Wu, S. Chong, J. Benner, X. Liu, and M. Xu Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation Gene 231 1999 1 13
11. M. Xu, H. Paulus, and S. Chong Fusions to self-splicing inteins for protein purification Methods Enzymol. 362 2000 376 418
12. C. Morassutti, D.F. Amicis, B. Skerlavaj, M. Zanetti, and S. Marchetti Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system FEBS Lett. 519 2002 141 146
13. B.C. Stein, and I.R. Levin Natriuretic peptides: physiology, therapeutic potential, and risk stratification in ischemic heart disease Am. Heart J. 135 1998 914 923
14. M.R. Burger, and A.J. Burger BNP in decompensated heart failure: diagnostic, prognostic and therapeutic potential Curr. Opin. Invest. Drugs 2 2001 929 935
15. V. Baumanis, I. Jansone, A. Skangals, I. Mandrika, and V. Berzins Synthesis of recombinant atrial natriuretic peptide (rANP) using hybrid fusion protein phage fr coat/ANP (CP/ANP) Peptides 18 1997 1229 1235
16. H. Schagger, and G.V. Jagow Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range form 1 to 100 kDa Anal. Biochem. 166 1987 368 379
17. Y. Mitsuda, A. Takimoto, S. Kamitani, K. Kitamura, T. Sakata, and K. Mitsushima Large-scale production of functional human adrenomedullin: expression, cleavage, amidation, and purification Protein Express. Purif. 25 2002 448 455
18. H. Shimizu, K. Masuta, K. Aono, H. Asada, K. Sasakura, M. Tamaki, K. Sugita, and K. Yamada Molecular forms of human brain natriuretic peptide in plasma Clin. Chim. Acta 316 2002 129 135