The mechanism of protein splicing and its modulation by mutation

EMBO Journal

Volumn 15, Issue 19, 1996, Pages 5146-5153

  Xu, Ming Qun ^a   Perler, Francine B ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

Hydroxylamine; Intein; Protein engineering; Protein splicing; Thermophile

Indexed keywords

PROTEIN PRECURSOR;

ARTICLE; COVALENT BOND; GENETIC CONSERVATION; GENETIC ENGINEERING; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN MODIFICATION; RNA SPLICING;

EID: 0029838842     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00898.x     Document Type: Article

References (28)

1. Bolanowski, M.A., Earles, B.J. and Lennarz, W.J. (1984) Fatty acylation of proteins during development of sea urchin embryos. J. Biol. Chem., 259, 4934-4940.
2. Bruice, T.C. and Benkovic, S.J. (1966) Bioorganic Mechanisms. W.A.Benjamin, New York, Vol. I, pp. 268-294.
3. Clarke, N.D. (1994) A proposed mechanism for the self-splicing of proteins. Proc. Natl Acad. Sci. USA, 91, 11084-11088.
4. Cooper, A.A., Chen, Y., Lindorfer, M.A. and Stevens, T.H. (1993) Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision. EMBO J., 12, 2575-2583.
5. Davis, E.O., Sedgwick, S.G. and Colston, M.J. (1991) Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product. J. Bacteriol., 173, 5653-5662.
6. Davis, E.O., Jenner, P.J., Brooks, P.C., Colston, M.J. and Sedgwick, S.G. (1992) Protein splicing in the maturation of M. Tuberculosis RecA protein: a mechanism for tolerating a novel class of intervening sequence. Cell, 71, 201-210.
7. Davis, E.O., Thangaraj, J.S., Brooks, P.C. and Colston, M.J. (1994) Evidence of selection for protein introns in the RecAs of pathogenic mycobacteria. EMBO J., 13, 699-703.
8. Dawson, P.E., Muir, T.W., Clark-Lewis, I. and Kent, S.B. (1994) Synthesis of proteins by native chemical ligation. Science, 266, 776-779.
9. Gimble, F.S. and Thorner, J. (1992) Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae. Nature, 357, 301-306.
10. Gu, H.H., Xu, J., Gallagher, M. and Dean, G.E. (1993) Peptide splicing in the vacuolar ATPase subunit A from Candida tropicalis. J. Biol. Chem., 268, 7372-7381.
11. Guan, C., Cui, T., Rao, V., Liao, W., Benner, J., Lin, C.L. and Comb, D. (1996) Activation of glycosylasparaginase. Formation of active N-terminal threonine by intramolecular autoproteolysis. J. Biol. Chem., 271, 1732-1737.
12. +-ATPase, inhibit its processing by protein splicing. Biochem. Biophys. Res. Commun., 188, 40-47.
13. +-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J. Biol. Chem., 265, 6726-6733.
14. Hodges, R.A., Perler, F.B., Noren, C.J. and Jack, W.E. (1992) Protein splicing removes intervening sequences in an archaea DNA polymerase. Nucleic Acids Res., 20, 6153-6157.
15. Jencks, W.P., Cordes, S. and Carriulos, J. (1960) The free energy of thiol ester hydrolysis. J. Biol. Chem., 235, 3608-2314.
16. +-adenosine triphosphatase. Science, 250, 651-657.
17. Lambowitz, A.M. and Belfort, M. (1993) Introns as mobile genetic elements. Annu. Rev. Biochem., 62, 587-622.
18. Perler, F.B. et al. (1992) Intervening sequences in an Archaea DNA polymerase gene. Proc. Natl Acad. Sci. USA, 89, 5577-5581.
19. Perler, F.B., Davis, E.O., Dean, G.E., Gimble, F.S., Jack, W.E., Neff, N., Noren, C.J., Thorner, J. and Belfort, M. (1994) Protein splicing elements: inteins and exteins - a definition of terms and recommended nomenclature. Nucleic Acids Res., 22, 1125-1127.
20. Pietrokovski, S. (1994) Conserved sequence features of inteins (protein introns) and their use in identifying new inteins and related proteins. Protein Sci., 3735, 2340-2350.
21. Porter, J.A., Kessler, D.P., Ekker, S.C. Young, K.E., Lee, J.J., Moses, K. and Beachy, P.A. (1995) The product of hedgehog autoproteolytic cleavage active in local and long-range signalling. Nature, 374, 363-366.
22. Shao, Y., Xu, M. and Paulus, H. (1995) Protein splicing: Characterization of the aminosuccinimide residue at the carboxyl terminus of the excised intervening sequence. Biochemistry, 34, 10844-10850.
23. Shao, Y., Xu, M. and Paulus, H. (1996) Protein splicing: Evidence for an N-O acyl rearrangement as the initial step in the splicing process. Biochemistry, 35, 3810-3815.
24. Van Poelje, P.D. and Snell, E.E. (1990) Cloning, sequencing, expression, and site-directed mutagenesis of the gene from Clostridium perfringens encoding pyruvoyl-dependent histidine decarboxylase. Biochemistry, 29, 132-139.
25. Vanderslice, P., Copeland, W.C. and Robertus, J.D. (1988) Site-directed alteration of serine 82 causes nonproductive chain cleavage in prohistidine decarboxylase. J. Biol. Chem., 263, 10583-10586.
26. Wallace, C.J. (1993) The curious case of protein splicing: Mechanistic insights suggested by protein semisynthesis. Protein Sci., 2, 697-705.
27. Xu, M., Southworth, M.W., Mersha, F.B., Hornstra, L.J. and Perler, F.B. (1993) In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell, 75, 1371-1377.
28. Xu, M., Comb, D.G., Paulus, H., Noren, C.J., Shao, Y. and Perler, F.B. (1994) Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. EMBO J., 13, 5517-5522.