Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Gene

Volumn 67, Issue 1, 1988, Pages 31-40

  Smith, Donald B ^a   Johnson, Kevin S ^b  

^a ROYAL MELBOURNE HOSPITAL   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Affinity chromatography; expression vector; plasmid; Plasmodium falciparum; protease; recombinant DNA; Schistosoma japonicum

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; POLYPEPTIDE;

ANIMAL CELL; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; HEREDITY; METHODOLOGY; NONHUMAN; PLASMODIUM FALCIPARUM; PROTOZOON; SCHISTOSOMA JAPONICUM; TREMATODE;

AMINO ACID SEQUENCE; ANIMAL; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, STRUCTURAL; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SCHISTOSOMA JAPONICUM; SUPPORT, NON-U.S. GOV'T;

EID: 0023806075     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(88)90005-4     Document Type: Article

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