Single step intein-mediated purification of hGMCSF expressed in salt-inducible E. coli

Biotechnology Letters

Volumn 31, Issue 5, 2009, Pages 659-664

  Srinivasa Babu, K ^a   Muthukumaran, T ^a   Antony, Aju ^a   Prem Singh Samuel, S D ^a   Balamurali, M ^a   Murugan, V ^a   Meenakshisundaram, S ^a  

^a ANNA UNIVERSITY   (India)

Author keywords

Escherichia coli GJ1158; Fusion protein expression; Granulocyte macrophage colony stimulating factor; Intein

Indexed keywords

AFFINITY COLUMNS; CYTOKINE; E. COLI; ENZYMATIC CLEAVAGES; FUSION PROTEIN EXPRESSION; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; HUMAN GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTORS; INCLUSION BODIES; INTEIN; N TERMINALS; SINGLE STEPS; SPECIFIC ACTIVITIES;

MACROPHAGES; PURIFICATION;

ESCHERICHIA COLI;

DITHIOTHREITOL; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; HYBRID PROTEIN; INTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY;

CHROMATOGRAPHY, AFFINITY; DITHIOTHREITOL; ESCHERICHIA COLI; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; INTEINS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 65049091114     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-9921-8     Document Type: Article

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