Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin

Protein Expression and Purification

Volumn 37, Issue 2, 2004, Pages 361-367

  Guo, Chenyun ^a   Li, Zhuoyu ^a   Shi, Yawei ^a   Xu, Mingqun ^b   Wise, John G ^c   Trommer, Wolfgong E ^c   Yuan, Jingming ^a  

^a SHANXI UNIVERSITY   (China)

^b NEW ENGLAND BIOLABS   (United States)

^c Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

Bioactivity; Gelonin; In vitro Refolding; Intein; One step purification; Self cleavage

Indexed keywords

GEL PROTEIN, GELONIUM MULTIFLORUM; HYBRID PROTEIN; INTEIN; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ANIMAL; ARTICLE; BIOCHEMISTRY; CHEMICAL MODEL; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYMOLOGY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IC 50; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PH; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; RETICULOCYTE; WESTERN BLOTTING;

ANIMALS; BIOCHEMISTRY; BLOTTING, WESTERN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; INHIBITORY CONCENTRATION 50; INTEINS; ISOPROPYL THIOGALACTOSIDE; MODELS, CHEMICAL; PLANT PROTEINS; PLASMIDS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; RETICULOCYTES;

ESCHERICHIA COLI;

EID: 4444274531     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.06.037     Document Type: Article

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