Applications of novel affinity cassette methods: Use of peptide fusion handles for the purification of recombinant proteins

Journal of Molecular Recognition

Volumn 14, Issue 6, 2001, Pages 323-369

  Hearn, Milton T W ^a   Acosta, Danilo ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Biotin avidin systems; Calmodulin binding proteins; Carbohydrate binding proteins; Cloning methods; Immunoaffinity 'tags'; Peptide 'tag' cleavage; Peptide 'tags'; Peptide libraries; Phage display; Photocleaveable affinity systems; Polyionic 'tags'; Recombinant fusion proteins; Vector selection

Indexed keywords

BACTERIAL PROTEIN; BIOTIN; HISTIDINE; HYBRID PROTEIN; MALTOSE; PEPTIDE LIBRARY; RECOMBINANT PROTEIN; STAPHYLOCOCCUS PROTEIN A; STREPTAVIDIN; STREPTOCOCCAL IGG FC BINDING PROTEIN; STREPTOCOCCAL IGG FC-BINDING PROTEIN;

AFFINITY CHROMATOGRAPHY; AFFINITY LABELING; AMINO ACID SEQUENCE; ANTIBODY AFFINITY; BACTERIOPHAGE; BINDING SITE; CELL INCLUSION; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PROTEIN ENGINEERING; PROTEIN FOLDING; REVIEW;

AFFINITY LABELS; AMINO ACID SEQUENCE; ANTIBODY AFFINITY; BACTERIAL PROTEINS; BACTERIOPHAGES; BINDING SITES; BIOTIN; CHROMATOGRAPHY, AFFINITY; HISTIDINE; INCLUSION BODIES; MALTOSE; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; STAPHYLOCOCCAL PROTEIN A; STREPTAVIDIN; SUPPORT, NON-U.S. GOV'T;

EID: 0035542874     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/jmr.555     Document Type: Review

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206. Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper (II) affinity chromatography
207. Hard and soft acids and bases
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274. Expression and purification of the cytoplasmic tail of an endocytic receptor by fusion to a carbohydrate-recognition domain
275. Affinity purification of fusion chaperonin Cpn60-(His)(6) from thermophilic bacterium Bacillus strain MS and its use in facilitating protein refolding and preventing heat denaturation
276. Recovery of therapeutic from inclusion bodies: Problems and process strategies
277. Photocleavable biotinylated ligands for affinity chromatography
278. Product modification to assist isolation (facilitated processing)
279. A novel method of affinity-purifying proteins using a bis-arsenical fluorescein
280. Cu(II)-binding properties of a cytochrome c with a synthetic metal-binding site: His-X3-His in an alpha-helix
281. Engineering a de novo-designed coiled-coil heterodimerization domain off the rapid detection, purification and characterization of recombinantly expressed peptides and proteins
282. Effects of side-chain characteristics on stability and oligomerization state of a de novo-designed model coiled-coil: 20 amino acid substitutions in position 'd'
283. Purification of a rat neurotensin receptor in Escherichia coli
284. + -dependent glucose cotransporter (SGLT): Maintenance of surface expression and global transport function with selective pertubation of transport kinetics and polarized expression
285. Gene fusions for purpose of expression: An introduction
286. Gene fusion vectors based on the gene for staphylococcal protein A
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288. One-step purification of hybrid proteins which have β-galactosidase activity
289. Gene fusions with human carbonic anhydrase II for efficient expression and rapid single-step recovery of recombinant proteins: Expression of the Escherichia coli F1-ATPase epsilon subunit
290. Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification
291. Purification and characterization of recombinant human insulin-like growth factor II (IGF-II) expressed as a secreted fusion protein in Escherichia coli
292. Efficient and rapid affinity purification of proteins using recombinant fusion proteases
293. Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein
294. BTag: A novel six-residue epitope tag for surveillance and purification of recombinant proteins
295. Expression of equine morbillivirus (EMV) matrix and fusion proteins and their evaluation as diagnostic reagents
296. In vivo biotinylated recombinant antibodies: Construction, characterisation, and application of a bifunctional Fab-BCCP fusion protein produced in Escherichia coli
297. Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor
298. Expression and purification of recombinant neurotensin in Escherichia coli
299. The structure of an antigenic determinant in a protein
300. Bacteriophage display libraries
301. Purification of recombinant insect transferrin from large volumes of cell culture medium using high capacity Ni(2+)-dipicolylamine gel
302. A mammalian expression vector for the expression of GAL4 fusion proteins with an epitope tag and histidine tail
303. A universal expression-purification system based on the coiled-coil interaction of myosin heavy chain
304. Hexahistidine (His6)-tag dependent protein dimerization: A cautionary tale
305. An Escherichia coli expression vector that allows recovery of proteins with native N-termini from purified calmodulin-binding peptide fusions
306. Preparation of highly purified hemoglobin by affinity elution
307. Immunoadsorption: Strategies for antigen elution and production of reusable adsorbents
308. A new system for the expression of human interleukin-6 in Escherichia coli
309. Purification and reconstitution of murine mitochondrial glycerol-3-phosphate acyltransferase. Functional expression in baculovirus-infected insect cells
310. Epitope Tagging
311. 8-hydroxyquinoline-metal chelate chromatographic support: An additional mode of selectivity in immobilised metal affinity chromatography
312. Protein selectivity in immobilised metal affinity chromatography based on the surface accessibility of aspartic and glutamic acid residues
313. Application of immobilized metal ion chelate complexes as pseudocation exchange adsorbents for protein separation
314. Adsorption and selectivity characteristics of several human serum proteins with immobilised hard Lewis metal ion-chelate adsorbents
315. O-phosphoserine as a new chelating ligand for use with hard Lewis metal ions in the immobilized-metal affinity chromatography of proteins
316. Optimisation of a potentiometric system for the determination of acid-base and metal ion binding properties of immobilised metal ion affinity chromatographic adsorbents
317. Characterization by potentiometric procedures of the acid-base metal binding properties of two new classes of immobilized metal ion affinity adsorbents developed for protein purification
318. Suitability of immobilized metal affinity chromatography for protein purification from canola
319. Genetically fused protein A-luciferase for immunological blotting analyses
320. Polyelectrolyte precipitation of beta-galactosidase fusions containing poly-aspartic acid tails
321. Colocalization of the homophilic binding site and the neuritogenic activity of the cell adhesion molecule L1 to its second Ig-like domain
322. A new expression vector for high level protein production, one step purification and direct isotopic labelling of calmodulin-binding peptide fusion proteins
323. Strep-tag II for one-step affinity purification of active bHLHzip domain of human c-Myc