Mutagenesis of morphinone reductase induces multiple reactive configurations and identifies potential ambiguity in kinetic analysis of enzyme tunneling mechanisms

Journal of the American Chemical Society

Volumn 129, Issue 45, 2007, Pages 13949-13956

  Pudney, Christopher R ^a   Hay, Sam ^a   Pang, Jiayun ^a,b   Costello, Claire ^a   Leys, David ^a   Sutcliffe, Michael J ^a,b   Scrutton, Nigel S ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDES; COMPUTER SIMULATION; CRYSTAL STRUCTURE; ENZYME KINETICS; HYDROGEN BONDS; MOLECULAR DYNAMICS;

ENZYME TUNNELING; FLOW SPECTROSCOPY; HYDRIDE TRANSFER; NICOTINAMIDES;

COENZYMES;

MORPHINONE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; CHEMICAL REACTION KINETICS; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENZYME KINETICS; HYDROGEN BOND; MOLECULAR DYNAMICS; MUTAGENESIS; SPECTROPHOTOMETER;

BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; HYDROGEN BONDING; KINETICS; MODELS, CHEMICAL; MODELS, MOLECULAR; NAD; OXIDOREDUCTASES;

EID: 36148973895     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja074463h     Document Type: Article

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