Conformation coupled enzyme catalysis: Single-molecule and transient kinetics investigation of dihydrofolate reductase

Biochemistry

Volumn 44, Issue 51, 2005, Pages 16835-16843

  Antikainen, Nina M ^a   Smiley, R Derike ^b   Benkovic, Stephen J ^a   Hammes, Gordon G ^b  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYST ACTIVITY; ENERGY TRANSFER; ENZYME KINETICS; FLUORESCENCE; MOLECULES;

ENZYME CATALYSIS; HYDRIDE TRANSFER; ISOTOPE EFFECT;

ENZYMES;

AMINO ACID; CYSTEINE; DIHYDROFOLATE REDUCTASE; FLUORESCENT DYE; GLYCINE; MUTANT PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; VALINE;

ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; FLUORESCENCE; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

ALGORITHMS; BIOTINYLATION; CATALYSIS; CYSTEINE; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; FOLIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; LEAST-SQUARES ANALYSIS; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; MUTATION; NADP; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; STATISTICAL DISTRIBUTIONS; TETRAHYDROFOLATE DEHYDROGENASE;

EID: 29344466944     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051378i     Document Type: Article

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