Multiple states of the Tyr318Leu mutant of dihydroorotate dehydrogenase revealed by single-molecule kinetics

Journal of the American Chemical Society

Volumn 126, Issue 22, 2004, Pages 6914-6922

  Shi, Jue ^a   Palfey, Bruce A ^a,b   Dertouzos, Joe ^a   Jensen, Kaj Frank ^c   Gafni, Ari ^a,b   Steel, Duncan ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYMES; ESCHERICHIA COLI; FLUORESCENCE; MEMBRANES; SPECTROSCOPY; VAN DER WAALS FORCES;

FLUORESCENCE SPECTROSCOPY; TYROSINE;

AROMATIC HYDROCARBONS;

DIHYDROOROTATE DEHYDROGENASE; DIHYDROOROTIC ACID DERIVATIVE; FLAVINE MONONUCLEOTIDE; FLAVOPROTEIN; OROTIC ACID; TRITON X 100; TYROSINE;

ARTICLE; BINDING SITE; CATALYSIS; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ESCHERICHIA COLI; FLUORESCENCE; OXIDATION;

BINDING SITES; CATALYSIS; ESCHERICHIA COLI; FLAVIN MONONUCLEOTIDE; KINETICS; LEUCINE; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTATION; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE; TYROSINE;

EID: 3042732323     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja038902y     Document Type: Article

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