Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells

Nucleic Acids Research

Volumn 35, Issue 19, 2007, Pages 6598-6610

  Vickers, Timothy A ^a   Lima, Walt F ^a   Nichols, Josh G ^a   Crooke, Stanley T ^a  

^a ISIS PHARMACEUTICALS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; ARGONAUTE2 PROTEIN; GENE PRODUCT; MESSENGER RNA; RNA; RNA INDUCED SILENCING COMPLEX; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MAMMAL CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA CLEAVAGE; RNA DEGRADATION; RNA INTERFERENCE; TIME;

CELL LINE; EUKARYOTIC INITIATION FACTOR-2; HELA CELLS; HUMANS; KINETICS; RIBONUCLEASE III; RNA INTERFERENCE; RNA, SMALL INTERFERING; RNA-INDUCED SILENCING COMPLEX;

MAMMALIA;

EID: 36148972467     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkm663     Document Type: Article

References (36)

1. Fire,A., Xu,S., Montgomery,M.K., Kostas,S.A., Driver,S.E. and Mello,C.C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391, 806-811.
2. Zamore,P.D., Tuschl,T., Sharp,P.A. and Bartel,D.P. (2000) RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell, 101, 25-33.
3. Gregory,R.I., Chendrimada,T.P., Cooch,N. and Shiekhattar,R. (2005) Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. Cell, 123, 631-640.
4. Tomari,Y. and Zamore,P.D. (2005) Perspective: Machines for RNAi. Genes Dev., 19, 517-529.
5. Hammond,S.M., Caudy,A.A. and Hannon,G.J. (2001) Post-transcriptional gene silencing by double-stranded RNA. Nat. Rev. Genet., 2, 110-119.
6. Okamura,K., Ishizuka,A., Siomi,H. and Siomi,M.C. (2004) Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. Genes Dev., 18, 1655-1666.
7. Meister,G., Landthaler,M., Patkaniowska,A., Dorsett,Y., Teng,G. and Tuschl,T. (2004) Human argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol. Cell, 15, 185-197.
8. Song,J.-J., Smith,S.K., Hannon,G.J. and Joshua-Tor,L. (2004) Crystal structure of argonaute and its implications for RISC slicer activity. Science, 305, 1434-1437.
9. Rivas,F.V., Tolia,N.H., Song,J.-J., Aragon,J.P., Liu,J., Hannon,G.J. and Joshua-Tor,L. (2005) Purified argonaute2 and an siRNA form recombinant human RISC. Nat. Struct. Mol. Biol., 12, 340-349.
10. Holen,T., Amarzguioui,M., Babaie,E. and Prydz,H. (2003) Similar behaviour of single-strand and double-strand siRNAs suggests they act through a common RNAi pathway. Nucleic Acids Res., 31, 2401-2407.
11. McManus,M.T., Haines,B.B., Dillon,C.P., Whitehurst,C.E., Parijs,L.V., Chen,J. and Sharp,P.A. (2002) Small interfering RNA-mediated gene silencing in T Lymphocytes. J. Immunol., 169, 5754-5760.
12. Koller,E., Propp,S., Murray,H., Lima,W., Bhat,B., Swayze,E.E., Marcusson,E.G. and Dean,N.M. (2006) Competition for RISC binding predicts in vitro potency of siRNA. Nucleic Acids Res., 34, 4467-4476.
13. Baker,B.F., Lot,S.S., Condon,T.P., Cheng-Flournoy,S., Lesnik,E.A., Sasmor,H.M. and Bennett,C.F. (1997) 2′-O-(2-methoxy)ethyI-modified anti-intercellular adhesion molecule 1 (ICAM-1) oligonucleotides selectively increase the ICAM-1 mRNA level and inhibit formation of the ICAM-1 translation initiation complex in human umbilical vein endothelial cells. J. Biol. Chem., 272, 11994-12000.
14. Vickers,T.A., Zhang,H., Graham,M.J., Lemonidis,K.M., Zhao,C. and Dean,N.M. (2006) Modification of MyD88 mRNA splicing and inhibition of IL-1beta signaling in cell culture and in mice with a 2′-O-methoxyethyl-modified oligonucleotide. J. Immunol., 176 3652-3661.
15. Winer,J., Kwang,C., Jung,S., Shackel,I. and Williams,P.M. (1999) Development and validation of real-time quantitative reverse transcriptase±polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal. Biochem., 270, 41-49.
16. Hashimoto,J.G., Beadles-Bohling,A.S. and Wiren,K.M. (2004) Comparison of ribogreen and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis. Biotechniques, 36, 58-60.
17. Doi,N., Zenno,S., Ueda,R., Ohki-Hamazaki,H., Ui-Tei,K. and Saigo,K. (2003) Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Curr. Biol. 13, 41-46.
18. Liu,J., Carmell,M.A., Rivas,F.V., Marsden,C.G., Thomson,J.M., Song,J.-J., Hammond,S.M., Joshua-Tor,L. and Hannon,G.J. (2004) Argonaute2 is the catalytic engine of mammalian RNAi. Science, 305, 1437-1441.
19. Wu,H., Lima,W.F. and Crooke,S.T. (1999) Properties of cloned and expressed human RNase H1. J. Biol. Chem., 274, 28270-28278.
20. Chendrimada,T.P., Gregory,R.I., Kumaraswamy,E., Norman,J., Cooch,N., Nishikura,K. and Shiekhattar,R. (2005) TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. Nature, 436 740-744.
21. Kanellopoulou,C., Muljo,S.A., Kung,A.L., Ganesan,S., Drapkin,R., Jenuwein,T., Livingston,D.M. and Rajewsky,A.K. (2005) Dicer-deficient mouse embryonic stem cells are defective in differentiation and centrorneric silencing. Genes Dev., 19, 489-501.
22. Murchison,E.P., Partridge,J.F., Tam,O.H., Cheloufi,S. and Hannon,G.J. (2005) Characterization of Dicer-deficient murine embryonic stem cells. Proc. Natl Acad. Sci. USA, 102, 12135-12140.
23. Kim,D.-H., Behlke,M.A., Rose,S.D., Chang,M.-S., Choi,S. and Rossi,J.J. (2004) Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy. Nat. Biotechnol., 23, 222-226.
24. Paddison,P.J., Caudy,A.A. and Hannon,G.J. (2002) Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl Acad. Sci. USA 99, 1443-1448.
25. Martinez,J., Patkaniowska,A., Urlaub,H., Lührmann,R. and Tuschl,J. (2002) Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. Cell, 110, 563-574.
26. Yi,R., Doehle,B.P., Qin,Y., Macara,I.G. and Cullen,B.R. (2005) Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs. RNA, 11, 220-226.
27. Ohrt,T., Merkle,D., Birkenfeld,K., Echeverri,C.J. and Schwille,P. (2006) In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5. Nucleic Acids Res., 34, 1369-1380.
28. Grimm,D., Streetz,K.L., Jopling,C.L., Storm,T.A., Pandey,K., Davis,C.R., Marion,P., Salazar,F. and Kay,M.A. (2006) Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature, 441, 537-541.
29. Meister,G. and Tuschl,T. (2004) Mechanisms of gene silencing by double-stranded RNA. Nature, 431, 343-349.
30. Holen,T. (2005) Mechanisms of RNAi: MRNA cleavage fragments may indicate stalled RISC. Journal of RNAi and Gene Silencing, 1, 21-25.
31. Liu,J., Valencia-Sanchez,M.A., Hannon,G.J. and Parker,R. (2005) MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell Biol., 7, 719-723.
32. Haley,B. and Zamore,P.D. (2004) Kinetic analysis of the RNAi enzyme complex. Nat. Struct. Mol. Biol., 11, 599-606.
33. Tomari,Y., Matranga,C., Haley,B., Martinez,N. and Zamore,P.D. (2004) A protein sensor for siRNA asymmetry. Science, 306, 1377-1380.
34. Rossi,J.J. (2005) RNAi and the P-body connection. Nat. Cell Biol., 7, 643-644.
35. Vickers,T.A., Koo,S., Bennett,C.F., Crooke,S.T., Dean,N.M. and Baker,B.F. (2003) Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents. J. Biol. Chem., 278, 7108-7118.
36. Oberdoerffer,P., Kanellopoulou,C., Heissmeyer,V., Paeper,C., Borowski,C., Aifantis,I., Rao,A. and Rajewsky,K. (2005) Efficiency of RNA interference in the mouse hematopoietic system varies between cell types and developmental stages. Mol. Cell. Biol., 25, 3896-3905.