Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro

Analytical Biochemistry

Volumn 270, Issue 1, 1999, Pages 41-49

  Winer, Jane ^a   Jung, Christopher Kwang S ^a   Shackel, Irene ^a   Williams, P Mickey ^a  

^a GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE; GENE EXPRESSION; MUSCLE; PATHOLOGY; PLANTS (BOTANY); RNA;

ATRIAL NATRIURETIC PEPTIDES; BIOLOGICAL TREATMENT; FLUORESCENT EMISSION; MRNA EXPRESSION LEVEL; NORTHERN BLOT ANALYSIS; REAL-TIME QUANTITATIVE RT-PCR; REVERSE TRANSCRIPTASE- POLYMERASE CHAIN REACTION; STANDARD CURVE METHOD;

POLYMERASE CHAIN REACTION;

ATRIAL NATRIURETIC FACTOR; ENDOTHELIN; LEUKEMIA INHIBITORY FACTOR; MESSENGER RNA; PHENYLEPHRINE; POLYADENYLIC ACID; PROSTAGLANDIN F2 ALPHA; PROTEIN C FOS;

ACCURACY; ANIMAL CELL; ARTICLE; ARTIFACT REDUCTION; CONCENTRATION RESPONSE; CONTROLLED STUDY; GENE EXPRESSION; HEART MUSCLE CELL; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN INDUCTION; QUANTITATIVE ASSAY; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; VALIDATION PROCESS;

ANIMALIA;

EID: 0033562350     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4085     Document Type: Article

References (15)

1. Heid C. A., Stevens J., Livak K. J., Williams P. M. Genome Res. 6:1996;986-994.
2. Gibson U. E., Heid C. A., Williams P. M. Genome Res. 6:1996;995-1001.
3. Wittwer C. T., Herrmann M. G., Moss A. A., Rasmussen R. P. Biotechniques. 22:1997;130-131.
4. Simpson P., McGrath A., Savion S. Circ. Res. 51:1982;787-801.
5. Simpson P. C., Karns L. R., Long C. S. Molecular Biology of the Cardiovascular System. 1990;Lea and Febiger. p. 53-81.
6. King K. L., Lai J., Winer J., Luis E., Yen R., Hooley J., Williams P. M., Mather J. P. Endocrine. 5:1996;85-93.
7. Fujisaki H., Ito H., Hirata Y., Tanaka M., Hata M., Lin M., Adachi S., Akimoto H., Marumo F., Hiroe M. J. Clin. Invest. 96:1995;1059-1065.
8. Wollert K. C., Taga T., Saito M., Narazaki M., Kishimoto T., Glembotski C. C., Vernallis A. B., Heath J. K., Pennica D., Wood W. I., Chien K. R. J. Biol. Chem. 271:1996;9535-9545.
9. Sheng Z., Knowlton K., Chen J., Hoshijima M., Brown J. H., Chien K. R. J. Biol. Chem. 272:1997;5783-5791.
10. Ito H., Hirata Y., Hiroe M., Tsujino M., Adachi S., Takamoto T., Nitta M., Taniguchi K., Marumo F. Circ. Res. 69:1991;209-215.
11. Lai J., Jin H., Yang R., Winer J., Li W., Yen R., King K. L., Ziegler F., Ko A., Cheng J., Bunting S., Paoni N. F. Am. J. Physiol. 271:1996;H2197-H2208.
12. Shubeita H. E., McDonough P. M., Harris A. N., Knowlton K. U., Glembotski C. C., Brown J. H., Chien K. R. J. Biol. Chem. 265:1990;20555-20562.
13. Pennica D., Shaw K. J., Swanson T. A., Moore M. W., Shelton D. L., Zioncheck L. A., Rosenthal A., Taga T., Paoni N. F., Wood W. I. J. Biol. Chem. 270:1995;10915-10922.
14. Iwaki K., Sukhatme V. P., Shubeita H. E., Chien K. R. J. Biol. Chem. 265:1990;13809-13817.
15. Nakagawa O., Ogawa Y., Itoh H., Suga S., Komatsu Y., Kishimoto I., Nishino K., Yoshimasa T., Nakao K. J. Clin. Invest. 96:1995;1280-1287.