Short-interfering-RNA-mediated gene silencing in mammalian cells requires dicer and elF2C translation initiation factors

Current Biology

Volumn 13, Issue 1, 2003, Pages 41-46

  Doi, Noboru ^a   Zenno, Shuhei ^a   Ueda, Ryu ^b,c   Ohki Hamazaki, Hiroko ^d   Ui Tei, Kumiko ^e   Saigo, Kaoru ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b MITSUBISHI KAGAKU INSTITUTE OF LIFE SCIENCES   (Japan)

^c NATIONAL INSTITUTE OF GENETICS   (Japan)

^d TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^e NIPPON MEDICAL SCHOOL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

3T3 CELLS; AMINO ACID SEQUENCE; ANIMALS; ENDORIBONUCLEASES; EUKARYOTIC INITIATION FACTORS; GENE SILENCING; GENES, MYC; HELA CELLS; HUMANS; MACROMOLECULAR SUBSTANCES; MAMMALS; MICE; MOLECULAR SEQUENCE DATA; PEPTIDE INITIATION FACTORS; PRECIPITIN TESTS; PROTEIN BIOSYNTHESIS; RIBONUCLEASE III; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EUKARYOTA; MAMMALIA;

EID: 0037425583     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(02)01394-5     Document Type: Article

References (18)

1. Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., and Mello, C.C. (1998). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806-811.
2. Bernstein, E., Candy, A.A., Hammond, S.M., and Hannon, G.J. (2001). Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363-366.
3. Ui-Tei, K., Zenno, S., Miyata, Y., and Saigo, K. (2000). Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target. FEBS Lett. 479, 79-82.
4. Billy, E., Brondani, V., Zhang, H., Muller, U., and Filipowicz, W. (2001). Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines. Proc. Natl. Acad. Sci. USA 98, 14428-14433.
5. Paddison, P.J., Caudy, A.A., and Hannon, G.J. (2002). Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 1443-1448.
6. Yang, S., Tutton, S., Pierce, E., and Yoon, K. (2001). Specific double-stranded RNA interference in undifferentiated mouse embryonic stem cells. Mol. Cell. Biol. 21, 7807-7816.
7. Wianny, F., and Zernicka-Goetz, M. (1999). Specific interference with gene function by double-stranded RNA in early mouse development. Nat. Cell Biol. 2, 70-75.
8. Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. (2001). Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498.
9. Grishok, A., Pasquinelli, A.E., Conte, D., Li, N., Parrish, S., Ha, I., Baillie, D.L., Fire, A., Ruvkun, G., and Mello, C.C. (2001). Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 106, 23-34.
10. Tabara, H., Sarkissian, M., Kelly, W.G., Fleenor, J., Grishok, A., Timmons, L., Fire, A., and Mello, C.C. (1999). The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99, 123-132.
11. Hammond, S.M., Boettcher, S., Caudy, A.A., Kobayashi, R., and Hannon, G.J. (2001). Argonaute2, a link between genetic and biochemical analyses of RNAi. Science 293, 1146-1150.
12. Zou, C., Zhang, Z., Wu, S., and Osterman, J.C. (1998). Molecular cloning and characterization of a rabbit elF2C protein. Gene 211, 187-194.
13. Koesters, R., Adams, V., Betts, D., Moos, R., Schmid, M., Siermann, A., Hassam, S., Weitz, S., Lichter, P., Heitz, P.U., et al. (1999). Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression. Genomics 61, 210-218.
14. Lipardi, C., Wei, Q., and Paterson, B.M. (2001). RNAi as random degradative PCR: siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs. Cell 107, 297-307.
15. Sijen, T., Fleenor, J., Simmer, F., Thijssen, K.L., Parrish, S., Timmons, L., Plasterk, R.H.A., and Fire, A. (2001). On the role of RNA amplification in dsRNA-triggered gene silencing. Cell 107, 465-476.
16. Mourelatos, Z., Dostie, J., Paushkin, S., Sharma, A., Charroux, B., Abel, L., Rappsilber, J., Mann, M., and Dreyfull, G. (2002). miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. Genes Dev. 16, 720-728.
17. Niwa, H., Yamamura, K., and Miyazaki, J. (1991). Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193-200.
18. Matsuda, S., Ichigotani, Y., Okuda, T., Irimura, T., Nakatsugawa, S., and Hamaguchi, M. (2000). Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase. Biochim. Biophys. Acta 1490, 163-169.