Manipulation of the active site loops of D-hydantoinase, a (β/α)8-barrel protein, for modulation of the substrate specificity

Biochemistry

Volumn 43, Issue 23, 2004, Pages 7413-7420

  Cheon, Young Hoon ^a,c   Park, Hee Sung ^a   Kim, Jin Hyun ^a   Kim, Youngsoo ^b   Kim, Hak Sung ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Seoul National University   (South Korea)

^c CrystalGenomics Inc   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ENZYMES; MUTAGENESIS; SATURATION (MATERIALS COMPOSITION); STEREOCHEMISTRY; SUBSTITUTION REACTIONS; SYNTHESIS (CHEMICAL);

MUTANTS; STEREOCHEMISTRY GATE LOOPS (SGL); STRUCTURAL DETERMINANTS;

BIOCHEMISTRY;

AMIDASE; AMINO ACID; DEXTRO DIHYDROPYRIMIDINASE; DIHYDROPYRIMIDINASE; HYDANTOIN DERIVATIVE; HYDROXYPHENYLHYDANTOIN; LEUCINE; METHIONINE; MUTANT PROTEIN; PHENYLALANINE; UNCLASSIFIED DRUG;

AMINO ACID SYNTHESIS; ARTICLE; BACILLUS STEAROTHERMOPHILUS SD1; BINDING KINETICS; ENZYME ACTIVE SITE; ENZYME MANIPULATION; ENZYME MODIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GEOBACILLUS STEAROTHERMOPHILUS; HYDROPHOBICITY; MODULATION; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; STEREOCHEMISTRY; STEREOCHEMISTRY GATE LOOP; STRUCTURE ANALYSIS;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; AMINO ACIDS; BACILLUS STEAROTHERMOPHILUS; BINDING SITES; HYDANTOINS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 2942586825     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi036330o     Document Type: Article

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