Altering substrate specificity of phosphatidylcholine-preferring phospholipase C of Bacillus cereus by random mutagenesis of the headgroup binding site

Biochemistry

Volumn 42, Issue 6, 2003, Pages 1603-1610

  Antikainen, Nina M ^a   Hergenrother, Paul J ^a,b   Harris, Micheleen M ^a   Corbett, William ^a   Martin, Stephen F ^a  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; CATALYST ACTIVITY; ENZYME KINETICS; HYDROLYSIS; MUTAGENESIS; PHOSPHOLIPIDS; RATE CONSTANTS;

PHOSPHORYLATION;

BIOCHEMISTRY;

DIACYLGLYCEROL; GLUTAMINE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MUTANT PROTEIN; PHENYLALANINE; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLSERINE; PHOSPHOLIPASE C; PHOSPHOLIPID; PHOSPHOLIPID SUBSTRATE; PHOSPHOLIPID SUBSTRATE 6CPS; PHOSPHOLIPID SUBSTRATE C6PC; PHOSPHOLIPID SUBSTRATE C6PE; PROTEIN SUBUNIT; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS CEREUS; BINDING AFFINITY; BINDING KINETICS; BINDING SITE; CATALYSIS; CHEMICAL BINDING; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; RANDOMIZATION; REACTION OPTIMIZATION; RESIDUE ANALYSIS; SITE DIRECTED MUTAGENESIS; SOLUBILITY; STEADY STATE; STRUCTURE ACTIVITY RELATION;

AMINO ACID SUBSTITUTION; BACILLUS CEREUS; BINDING SITES; CARRIER PROTEINS; CATALYSIS; KINETICS; MALTOSE; MICELLES; MUTAGENESIS, SITE-DIRECTED; PEPTIDE LIBRARY; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; PHOSPHATIDYLSERINES; PHOSPHOLIPASE C; POLYMERASE CHAIN REACTION; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY;

BACILLUS CEREUS; POSIBACTERIA;

EID: 0037452525     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0267285     Document Type: Article

References (43)

1. Martin, S. F., Follows, B. C., Hergenrother, P. J., and Trotter, B. K. (2000) Biochemistry 39, 3410-3415.
2. Arnold, F. H. (2001) Nature 409, 253-257.
3. Harris, J. L., and Craik, C. S. (1998) Curr. Opin. Chem. Biol. 2, 127-132.
4. Shah, K., Liu, Y., Deirmengian, C., and Shokat, K. M. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 3565-3570.
5. Park, Y., Luo, J., Schultz, P. G., and Kirsch, J. F. (1997) Biochemistry 36, 10517-10525.
6. Srivastava, D. K., and Peterson, K. L. (1998) Biochemistry, 37, 8446-8456.
7. Czjzek, M., Cicek, M., Zamboni, V., Bevan, D. R., Henrissat, B., and Esen, A. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 13555-13560.
8. Penning, T. M., and Jez, J. M. (2001) Chem. Rev. 101, 3027-3046.
9. Hedstrom, L., Szilagyi, L., and Rutter, W. J. (1992) Science 225, 1249-1253.
10. Onuffer, J. J., and Kirsch, J. F. (1995) Protein Sci. 4, 1750-1757.
11. Wilks, H. M., Hart, K. W., Feeney, R., Dunn, C. R., Muirhead, H., Chia, W. N., Barstow, D. A., Atkinson, T., Clarke, A. R., and Holbrook, J. J. (1988) Science 242, 1541-1544.
12. Crameri, A., Dawes, G., Rodriguez, E., Silver, S., and Stemmer, W. P. C. (1997) Nature Biotech. 15, 436-438.
13. Moore, J. C., and Arnold, F. H. (1996) Nature Biotech. 14, 458-467.
14. Yano, T., Oue, S., and Kagamiyama, H. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 5511-5515.
15. Altamarino, M. M., Blackburn, J. M., Aguayo, C., Fersht, A. R. (2000) Nature 403, 617-622.
16. Hubner, B., Haensler, M., and Hahn, U. (1999) Biochemistry 38, 1371-1376.
17. Choo, Y., and Klug, A. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 11163-11167.
18. Huang, W., Petrosino, J., Hirsch, M., Shenkin, P. S., and Palzkill, T. (1996) J. Mol. Biol. 258, 688-703.
19. Graham, L. D., Hagget, K. D., Jennings, P. A., Le Brocque, D. S., and Whittaker, R. G. (1993) Biochemistry 32, 6250-6258.
20. Viadiu, H., Osuna, J., Fink, A. L., and Soberon, X. (1995) J. Biol. Chem. 270, 781-787.
21. Hergenrother, P. J., and Martin, S. F. (1997) Anal. Biochem. 251, 45-49.
22. Hergenrother, P. J., and Martin, S. F. (2001) Top. Curr. Chem. 211, 131-167.
23. Martin, S. F., Wong, Y.-L., and Wagman, A. S. (1994) J. Org. Chem. 59, 4821-4831.
24. Martin, S. F., Spaller, M. R., and Hergenrother, P. J. (1996) Biochemistry 35, 12970-12977.
25. Martin, S. F., and Hergenrother, P. J. (1998) Biochemistry 37, 5755-5760.
26. Martin, S. F., and Hergenrother, P. J. (1998) Bioorg. Med. Chem. Lett. 8, 593-596.
27. Martin, S. F., and Hergenrother, P. J. (1999) Biochemistry 38, 4403-4408.
28. Martin, S. F., Follows, B. C., Hergenrother, P. J., and Franklin, C. L. (2000) J. Org. Chem. 65, 4509-4514.
29. Exton, J. H. (1997) Eur. J. Biochem. 243, 10-20.
30. Guddal, P. H., Johansen, T., Schulstad, K., and Little, C. (1989) J. Bacteriol. 171, 5702-5706.
31. Hough, E., Hansen, L. K., Birkness, B., Jynge, K., Hansen, S., Hordvik, A., Little, C., Dodson, E., and Derewenda, Z. (1989) Nature 338, 357-360.
32. Hansen, S., Hough, E., Svensson, L. A., Wong, Y. L., and Martin, S. F. (1993) J. Mol. Biol. 234, 179-187.
33. Hansen, S., Hansen, L. K., and Hough, E. (1993) J. Mol. Biol. 231, 870-876.
34. Hansen, S., Hansen, L. K., and Hough, E. (1992) J. Mol. Biol. 225, 543-549.
35. Kearney, P. C., Mizoue, L. S., Kumpf, R. A., Forman, J. E., McCurdy, A., and Dougherty, D. A. (1993) J. Am. Chem. Soc. 115, 9907-9919.
36. Ma, J. C., and Dougherty, D. A. (1997) Chem. Rev. 97, 1303-1324.
37. Wouters, J. (1998) Protein Sci. 7, 2472-2475.
38. Basran, J., Mewies, M., Mathews, F. S., and Scrutton, N. S. (1997) Biochemistry 36, 1989-1998.
39. Dougherty, D. A. (1996) Science 271, 163-168.
40. Ting, A., Shin, I., Lucero, C., and Schultz, P. G. (1998) J. Am. Chem Soc. 120, 7135-7136.
41. We thank Professor Barbara S. Plake (Department of Educational Psychology, University of Nebraska at Lincoln) for this analysis.
42. Hergenrother, P. J. (1999) The Catalytic Mechanism of Phospholipase C and the Total Synthesis of Erythromycin B, Ph.D. Dissertation, University of Texas at Austin.
43. Hirel, P. H., Schmitter, J. M., Dessen, P., Fayat, G., and Balanquet, S. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 8247-8251.