Directed coevolution of stability and catalytic activity in calcium-free subtilisin

Biochemistry

Volumn 44, Issue 9, 2005, Pages 3272-3279

  Strausberg, Susan L ^a   Ruan, Biao ^a   Fisher, Kathryn E ^a   Alexander, Patrick A ^a   Bryan, Philip N ^a  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLATION; AMINO ACIDS; CALCIUM; MUTAGENESIS; OPTIMIZATION; RATE CONSTANTS;

HYPERSTABILITY; MUTATIONS; RANDOMIZATION; SUBTILISIN;

CATALYST ACTIVITY;

AMINO ACID; CALCIUM FREE SUBTILISIN; SUBTILISIN; UNCLASSIFIED DRUG;

ACYLATION; AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CLONE; COEVOLUTION; ENZYME KINETICS; ENZYME METABOLISM; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; RANDOMIZATION; SITE DIRECTED MUTAGENESIS; STEADY STATE; TEMPERATURE;

ACYLATION; BACILLUS; BACTERIAL PROTEINS; CALCIUM; CATALYSIS; CHROMOGENIC COMPOUNDS; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVATION; ENZYME STABILITY; KINETICS; MUTAGENESIS, SITE-DIRECTED; OLIGOPEPTIDES; PROTEIN BINDING; SUBTILISIN;

EID: 14644436356     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi047806m     Document Type: Article

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