Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification

Protein Engineering

Volumn 10, Issue 8, 1997, Pages 975-982

  Voss, Selma ^a   Skerra, Arne ^a  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Alkaline phosphatase; Aminobenzoic acid; Escherichia coli secretion; Filter sandwich assay; Tetracycline promoter

Indexed keywords

ALKALINE PHOSPHATASE; HYBRID PROTEIN; MUTANT PROTEIN; RECOMBINANT PROTEIN; STREPTAVIDIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; CHROMATOPHORE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ELECTROPHORETIC MOBILITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETIC ENGINEERING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN IMMOBILIZATION; PROTEIN PURIFICATION;

ESCHERICHIA COLI;

EID: 0030780364     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.8.975     Document Type: Article

References (26)

1. Bachmann,B.J. (1972) Bacteriol. Rev., 36, 525-557.
2. Green,M. (1975) Adv. Protein Chem., 29, 85-133.
3. Ellison,D., Hinton,J., Hubbard,S.J. and Beynon,R.J. (1995) Protein Sci., 4, 1337-1345.
4. Heller,S., Finn,T.P., Huber, J., Nishi,R., Geissen,M., Puschel,A.W. and Rohrer,H. (1995) Development, 121, 2681-2693.
5. Kleymann,G., Ostermeier,C., Ludwig,B., Skerra,A. and Michel,H. (1995a) Bio/Technology, 13, 155-160.
6. Kleymann,G., Ostermeier,C., Heitmann,K., Haase,W. and Michel,H. (1995b) J. Histochem. Cytochem., 43, 607-614.
7. Kurzban,G.P., Bayer,E.A., Wilchek,M. and Horowitz,P.M. (1991) J. Biol. Chem., 266, 14470-14477.
8. Meldal,M. and Breddam,K. (1991) Anal. Biochem., 195, 141-147.
9. Müller,H.N. and Skerra,A. (1994) Biochemistry, 33, 14126-14135.
10. Ostermeier,C., Iwata,S., Ludwig,B. and Michel,H. (1995) Nature Struct. Biol., 2, 842-846.
11. Sambrook,J., Fritsch,E.F. and Maniatis,T. (1989) Molecular Cloning. A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
12. Schmidt,T.G.M. and Skerra,A. (1993) Protein Engng, 6, 109-122.
13. Schmidt,T.G.M. and Skerra,A. (1994a) J. Chromatogr. A, 676, 337-345.
14. Schmidt,F.S. and Skerra,A. (1994b) Eur. J. Biochem., 219, 855-863.
15. Schmidt,T.G.M., Koepke,J., Frank,R. and Skerra,A. (1996) J. Mol. Biol., 255, 753-766.
16. Skerra,A. (1992) Nucleic Acids Res., 20, 3551-3554.
17. Skerra,A. (1994) Gene, 151, 131-135.
18. Skerra,A., Dreher,M. and Winter,G. (1991) Anal. Biochem., 196, 151-155.
19. Smith,G.P. (1991) Curr. Opin. Biotechnol., 2, 668-673.
20. Sowadski,J.M., Handschumacher,M.D., Krishna Murthy,H.M., Foster,B.A. and Wyckoff,H.W. (1985) J. Mol. Biol., 186, 417-433.
21. Sutamihardja,T.M., Ishikura,A., Naito,J. and Ishiguro,I. (1972) Chem. Pharm. Bull., 20, 2694-2700.
22. Tsiotis,G., Haase,W., Engel,A. and Michel,H. (1995) Eur. J. Biochem., 231, 823-830.
23. Weber,P.C., Ohlendorf,D.H., Wendoloski,J.J. and Salemme,F.R. (1989) Science, 243, 85-88.
24. Wu,P. and Brand,L. (1994) Anal. Biochem., 218, 1-13.
25. Yanisch-Perron,C., Vieira,J. and Messing,J. (1985) Gene, 33, 103-119.
26. Zopf,D. and Ohlson,S. (1990) Nature, 346, 87-88.