Production of a thermostable DNA polymerase by site-specific cleavage of a heat-eluted affinity fusion protein

Protein Expression and Purification

Volumn 9, Issue 1, 1997, Pages 125-132

  Gräslund, Torbjörn ^a   Nilsson, Joakim ^a   Lindberg, A Michael ^b   Uhlén, Mathias ^a   Nygren, Per Åke ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b LINNAEUS UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); COXSACKIEVIRUS; ENTEROVIRUS; THERMUS; THERMUS AQUATICUS;

EID: 0031079535     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0674     Document Type: Article

References (45)

1. 1. Lawyer, F. C., Stoffel, S., Saiki, R. K., Myambo, K, Drummond, R., and Gelfand, D. H. (1989) Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264, 6427-6437.
2. 2. Engelke, D. R., Krikos, A., Bruck., M., and Ginsburg, D. (1990) Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Anal. Biochem. 191, 396-400.
3. 3. Lawyer, F. C., Stoffel, S., Saiki, R. K., Chang, S.-Y., Landre, P. A., Abramson, R. D., and Gelfand, D. H. (1993) High-level expression, purification and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity. PCR Methods Appl. 2, 275-287.
4. 4. Pluthero, F. G. (1993) Rapid purification of high-activity Taq DNA polymerase. Nucleic Acids Res. 21, 4850-4851.
5. 5. Desai, U. J., and Pfaffle, P. K. (1995) Single-step purification of a thermostable DNA polymerase expressed in Escherichia coli. BioTechniques 19, 780-784.
6. 6. Grimm, E., and Arbuthnot, P. (1995) Rapid purification of recombinant Taq DNA polymerase by freezing and high temperature thawing of bacterial expression cultures. Nucleic Acids Res. 23, 4518-4519.
7. 7. Tabor, S., and Richardson, C. C. (1995) A single residue in DNA polymerases from Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy-and dideoxyribonucleotides. Proc. Natl Acad. Sci. USA 92, 6339-6343.
8. 8. Barnes, W. (1994) PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates. Proc. Natl. Acad. Sci. USA 91, 2216-2220.
9. 9. Barnes, W. M. (1992) The fidelity of Taq DNA polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112, 29-35.
10. 10. Flaschel, E., and Friehs, K. (1993) Improvements of downstream processing of recombinant proteins by means of genetic engineering methods. In "Biotechnology Advances" (Moo-Young, M., and Glick, B. R., Eds.), Vol. 11, pp. 31-78, Pergamon Press.
11. 11. Nygren, P.-Å., Ståhl, S., and Uhlén, M. (1994) Engineering proteins to facilitate bioprocessing. TIBTECH 12, 184-188.
12. 12. Jungbauer, A., and Boschetti, E. (1994) Manufacture of recombinant proteins with safe and validated chromatographic sorbents. J. Chromatogr. A 662, 143-179.
13. 13. Ng, P., and Mitra, G. (1994) Removal of DNA contaminants from therapeutic protein preparations. J. Chromatogr. A 658, 459-463.
14. 14. Hansson, M., Ståhl, S., Hjort, R., Uhlén, M., and Moks, T. (1994). Single-step recovery of a secreted recombinant protein by expanded bed adsorption. Bio / Technology 12, 285-288.
15. 15. Carter, P. (1990) Site-specific proteolysis of fusion proteins. In "Protein Purification: From Molecular Mechanism to Large-Scale Processes. (Am. Chem. Soc. Symp. Ser. No. 427)" (Ladish, M. R., Willson, R. C., Painton, C. C., and Builder, S. E., Eds.), pp. 181-193, American Chemical Society Press.
16. 16. Carter, P., and Wells, J. (1987) Engineering enzyme specificity by "substrate-assisted catalysis." Science 237, 394-399.
17. 17. Carter, P., Nilsson, B., Burnier, J. P., Burdick, D., and Wells, J. (1989) Engineering subtilisin BPN′ for site-specific proteolysis. Proteins 6, 240-248.
18. 18. Ballinger, M. D., Tom, J., and Wells, J. (1995) Designing subtilisin BPN′ to cleave substrates containing dibasic residues. Biochemistry 34, 13312-13319.
19. 19. Walker, P. A., Leong, L. E.-C., Ng, P. W. P., Han Tan, S., Waller, S., Murphy, D., and Porter, A. G. (1994) Efficient and rapid affinity purification of proteins using recombinant fusion proteases. Bio / Technology 12, 601-605.
20. 20. Pohlner, J., Klauser, T., Kuttler, E., and Halter, R. (1992) Sequence-specific cleavage of protein fusions using a recombinant Neisseria type 2 IgA protease. J. Biotechnol. 10, 799-804.
21. 21. Vozza, L. A., Wittwer, L., Higgins, D. R., Purcell, T. J., Bergseid, M., Collins-Racie, L. A., LaVallie, E. R., and Hoeffler, J. P. (1996) Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris. Bio / Technology 14, 77-81.
22. 22. Miyashita, K., Kusumi, M., Utsumi, R., Komano, T., and Satoh, N. (1992) Expression and purification of recombinant 3C proteinase of coxsackievirus. Biosci. Biotechnol. Biochem. 56, 746-750.
23. 23. Lindberg, M., Stålhandske, P. O. K., and Petterson, U. (1987) Genome of Coxsackievirus B3. Virology 156, 50-63.
24. 24. Miyashita, K., Kusumi, M., Utsumi, R., Katayama, S., Noda, M., Komano, T., and Satoh, N. (1993) Site-directed mutagenesis of the putitative active site residues of 3C proteinase of coxsackievirus B3: Evidence of a functional relationship with trypsin-like serine proteinases. Prot. Eng. 6, 189-193.
25. 25. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
26. 26. Rüther, U. (1982) pUR 250 allows rapid chemical sequencing of both strands of its insert. Nucleic Acids Res. 10, 5765-5772.
27. 27. Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes in "Methods in Enzymology" (Goedell, D. V., Ed.), Vol. 185, pp. 60-89, Academic Press, San Diego.
28. 28. Larsson, M., Brundell, E., Nordfors, L., Höög, C., Uhlén, M., and Ståhl, S. (1996) A general bacterial expression system for functional analysis of cDNA-encoded proteins. Protein Expr. Purif. 7, 447-457.
29. 29. Yanisch-Perron, C., Vieria, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.
30. 30. Hultman, T., Bergh, S., Moks, T., and Uhlén, M. (1991) Bidirectional solid phase sequencing of in vitro-amplified plasmid DNA. BioTechniques 10, 84-93.
31. 31. Nygren, P.-Å., Eliasson, M., Palmcrantz, E., Abrahmsén, L., and Uhlén, M. (1988) Analysis and use of the serum albumin binding region of streptococcal protein G. J. Mol. Recognit. 1, 69-74.
32. 32. Elango, N., Coligan, J. E., Jambou, R. C., and Venkatesan, S. (1986) Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: Nucleotide sequence of mRNA and limited amino acid sequence of the purified protein. J. Virol. 57, 481-489.
33. 33. Nilsson, J., Nilsson, P., Williams, Y., Pettersson, L., Uhlén, M., and Nygren, P.-Å. (1994) Competitive elution of protein A fusion proteins allows specific recovery under mild conditions. Eur. J. Biochem. 224, 103-108.
34. 34. Sjölander, A., Ståhl, S., and Perlmann, P. (1993) Bacterial expression systems based on protein A and protein G designed for the production of immunogens: Applications to Plasmodium falciparum malaria antigens. Immunomethods 2, 79-92.
35. 35. Nygren, P.-Å., Flodby, P., Andersson, R., Wigzell, H., and Uhlén, M. (1991) In vivo stabilization of a human recombinant CD4 derivative by fusion to a serum albumin binding receptor. In "Vaccines" (Lerner, R. A., Ed.), Vol. 91, pp. 363-368, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
36. 36. Makrides, S., Nygren, P.-Å., Andrews, B., Ford, P. J., Evans, K. S., Hayman, E. G., Adari, H., Levin, J., Uhlén, M., and Toth, C. A. (1996) Extended in vivo half-life of human soluble complement receptor type 1 fused to a serum albumin binding receptor. J. Pharm. Exp. Therapeut. 277, 534-542.
37. 37. Kraulis, P. J., Jonasson, P., Nygren, P.-Å., Uhlén, M., Jendeberg, L., Nilsson, B., and Kördel, J. (1996) The serum albumin-binding domain of streptococcal protein G is a three-helical bundle: a heteronuclear NMR study. FEBS Lett. 378, 190-194.
38. 38. Nilsson, B., Moks, T., Jansson, B., Abrahmsén, L., Elmblad, A., Holmgren, E., Henrichson, C., Jones, T. A., and Uhlén, M. (1987) A synthetic IgG-binding domain based on staphylococcal protein A. Prot. Eng. 1, 107-113.
39. 39. Nilsson, J., Jonasson, P., Samuelsson, E., Ståhl, S., and Uhlén, M. (1996) Integrated production of human insulin and its C-peptide. J. Biotechnol., 48, 241-250.
40. 40. Hopp, T. H., Prickett, K. S., Price, V. L., Libby, R. T., March, C. J., Ceretti, D. P., Urdal, D. L., and Conlon, P. J. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio / Technology 6, 1204-1210.
41. 41. Smith, D. B., and Johnson, K. S. (1988) Single-site purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
42. 42. Hochuli, E., Baanwarth, W., Döbeli, H., Gentz, R., and Stüber, D. (1988) Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio / Technology 6, 1321-1325.
43. 43. Guan, C.-D., Li, P., Riggs, P. D., and Inoye, H. (1988) Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67, 21-30.
44. 44. Adams, M. W. W., Perler, F. B., and Kelly, R. M. (1995) Extremozymes: expanding the limits of biocatalysis. Bio / Technology 13, 662-668.
45. 45. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.