Overexpression and large-scale purification of recombinant hamster polymorphic arylamine N-acetyltransferase as a dihydrofolate reductase fusion protein

Protein Expression and Purification

Volumn 10, Issue 1, 1997, Pages 141-153

  Sticha, Kristina R K ^a   Sieg, Christine A ^a   Bergstrom, Carl P ^a   Hanna, Patrick E ^a   Wagner, Carston R ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0031172320     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0734     Document Type: Article

References (37)

1. Vatsis K. P., Weber W. W., Bell D. A., Dupret J.-M., Evans D. A. P., Grant D. M., Hein D. W., Lin H. J., Meyer U. A., Relling M. V., Sim E., Suzuki T., Yamazoe Y. Nomenclature forN. Pharmacogenetics. 5:1995;1-17.
2. Hanna P. E. NON,O. Adv. Pharmacol. 27:1994;401-430.
3. Ambrosone C. B., Freudenheim J. L., Graham S., Marshall J. R., Vena J. E., Brasure J. R., Michalek A. M., Laughlin R., Nemoto T., Gillenwater K. A., Harrington A. M., Shields P. G. Cigarette smoking,N. JAMA. 276:1996;1494-1501.
4. Minchin R. F. Acetylation ofpN. Biochem. J. 307:1995;1-3.
5. Ward A., Summers M. J., Sim E. Purification of recombinant humanNE. coli. Biochem. Pharmacol. 49:1995;1759-1767.
6. Bergstrom C. P., Wagner C. R., Ann D. K., Hanna P. E. Hamster monomorphic arylamineNEscherichia coli. Protein Expression Purif. 6:1995;45-55.
7. Wagner C. R., Bergstrom C. P., Koning K. R., Hanna P. E. ArylamineNEscherichia coli, Drug Metab. Dispos. 24:1996;245-253.
8. Uhlen M., Forsberg G., Moks T., Hartmanis M., Nilsson B. Fusion proteins in biotechnology. Curr. Opin. Biotechnol. 3:1992;363-369.
9. Uhlen M., Moks T. Gene fusions for purpose of expression: An introduction. Methods Enzymol. 185:1990;129-143.
10. Ford C. F., Suominen I., Glatz C. E. Fusion tails for the recovery and purification of recombinant proteins. Protein Expression Purif. 2:1991;95-107.
11. Blakley R. L. Dihydrofolate reductase. Folates and Pterins. 1989;Wiley, New York. p. 191-253.
12. Takasuga A., Banba K., Yoshino K., Izutsu H., Iwakura M., Ohashi S. Efficient production of a small peptide by expression as a multimeric form fused with the dihydrofolate reductase affinity handle. J. Biochem. 112:1992;652-657.
13. Iwakura M., Furusawa K., Kokubu T., Ohashi S., Tanaka Y., Shimura Y., Tsuda K. Dihydrofolate reductase as a new "affinity handle" J. Biochem. 111:1992;37-45.
14. Iwakura M., Matthews C. R. Construction and characterization of a single polypeptide chain containing two enzymatically active dihydrofolate reductase domains. Protein Eng. 5:1992;791-796.
15. Wagner C. R., Huang Z., Singleton S. F., Benkovic S. J. Molecular basis for nonadditive mutational effects inEscherichia coli. Biochemistry. 34:1995;15671-15680.
16. Huang Z., Wagner C. R., Benkovic S. J. Nonadditivity of mutational effects at the folate binding site ofEscherichia coli. Biochemistry. 33:1994;11576-11585.
17. Hakes D. J., Dixon J. E. New vectors for high level expression of recombinant proteins in bacteria. Anal. Biochem. 202:1992;293-298.
18. Smith D. R., Calvo J. M. Nucleotide sequence of the E. coli gene coding for dihydrofolate reductase. Nucleic Acids Res. 8:1980;2255-2274.
19. Hanahan D. Studies on transformation ofEscherichia coli. J. Mol. Biol. 166:1983;557-580.
20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
21. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
22. Williams J. W., Morrison J. F., Duggleby R. G. Methotrexate, a high-affinity pseudosubstrate of dihydrofolate reductase. Biochemistry. 18:1979;2567-2573.
23. Ellis K. J., Morrison J. F. Buffers of constant ionic strength for studying pH-dependent processes. Methods Enzymol. 87:1982;405-426.
24. Blakley R. L. Crystalline dihydropteroylglutamic acid. Nature (London). 188:1960;231-232.
25. Booth J. Acetyl transfer in arylamine metabolism. Biochem. J. 100:1966;745-753.
26. Weber W. W. N. Methods Enzymol. 17B:1971;805-811.
27. Hein D. W., Omichinski J. G., Brewer J. A., Weber W. W. A unique pharmacogenetic expression of theN. J. Pharmacol. Exp. Ther. 220:1982;8-15.
28. Kawakubo Y., Manabe S., Yamazoe Y., Nishikawa T., Kato R. Properties of cutaneous acetyltransferase catalyzingNON. Biochem. Pharmacol. 37:1988;265-270.
29. Stone S. R., Morrison J. F. Mechanism of inhibition of dihydrofolate reductases from bacterial and vertebrate sources by various classes of folate analogs. Biochim. Biophys. Acta. 869:1986;275-285.
30. Andres H. H., Kolb H. J., Weiss L. Purification and physical-chemical properties of acetyl-CoA:arylamineN. Biochim. Biophys. Acta. 746:1983;182-192.
31. Andres H. H., Vogel R. S., Tarr G. E., Johnson L., Weber W. W. Purification, physicochemical, and kinetic properties of liver acetyl-CoA:arylamineN. Mol. Pharmacol. 31:1987;446-456.
32. Hatt J., Callahan M., Greener A. TOPP strains:E. coli. Strategies. 5:1992;2-3.
33. Kaufman B. T. Methotrexate-agarose in the purification of dihydrofolate reductase. Methods Enzymol. 34:1974;272-281.
34. Hein D. W., Kirlin W. G., Ferguson R. J., Weber W. W. Biochemical investigation of the basis for the genetic N-acetylation polymorphism in the inbred hamster. J. Pharmacol. Exp. Ther. 234:1985;358-364.
35. Cheon H. G., Boteju L. W., Hanna P. E. Affinity alkylation of hamster hepatic arylamineN. Mol. Pharmacol. 42:1992;82-93.
36. M. J. Wick, 1988, Characterization ofNN, University of Minnesota, Minneapolis.
37. Ozawa S., Abu-Zeid M., Kawakubo Y., Toyama S., Yamazoe Y., Kato R. Monomorphic and polymorphic isozymes of arylamineNN. Carcinogenesis. 11:1990;2137-2144.